The Adenine at the 4th Exon of the <i>DRO1</i> Gene Provides Drought-Tolerance Capacity to Hybrid Rice Deyou4727 and Its Maintainer Line Dexiang074B
Extreme weather and global warming are drastic agricultural issues causing water scarcity. Under these conditions, it is vital to select drought-tolerant hybrid super rice breeds to enhance the rice yield in dry areas. In the current study, a dry-shed experiment was carried out in 2019–2020 to analy...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-03-01
|
| Series: | Agronomy |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4395/12/3/752 |
| _version_ | 1827650274654683136 |
|---|---|
| author | Farhan Nabi Sumbal Sajid Guotao Yang Abdul Rasheed Kaleri Yating Zheng Jian Qin Xuechun Wang Kaifeng Jiang Youlin Peng Yungao Hu |
| author_facet | Farhan Nabi Sumbal Sajid Guotao Yang Abdul Rasheed Kaleri Yating Zheng Jian Qin Xuechun Wang Kaifeng Jiang Youlin Peng Yungao Hu |
| author_sort | Farhan Nabi |
| collection | DOAJ |
| description | Extreme weather and global warming are drastic agricultural issues causing water scarcity. Under these conditions, it is vital to select drought-tolerant hybrid super rice breeds to enhance the rice yield in dry areas. In the current study, a dry-shed experiment was carried out in 2019–2020 to analyze the root growth, gene expression, and enzyme activity of hybrid rice Deyou4727, its parent (Dexiang074B * Chenghui727), taking IR64 as a universal control. Results showed that Deyou4727 and its maintainer line Dexiang074B produced more roots (30–50 cm soil) and higher enzymatic activity than IR64 during the drought stress period, indicating a good drought-tolerant capacity for Deyou4727. The distribution and morphology of the root system in Deyou4727 were inherited by the maintainer line Dexiang074B, which was confirmed by the high upregulation of <i>DRO1</i> and <i>OsPIN1b</i> gene expression and the sharing of the same ORF region in both the hybrid rice varieties. However, the <i>DRO1</i> gene ORF study showed that, in the case of the control IR64, ‘A’ was missing at the 4th exon of the <i>DRO1</i> gene, which may explain the shallow and weak root architecture system of IR64. The drought-tolerance capacity of Deyou4727 can be used in the future as a drought-resistant germplasm for breeding more drought-tolerant hybrid rice varieties. |
| first_indexed | 2024-03-09T20:13:04Z |
| format | Article |
| id | doaj.art-e8028793b1cb45edb9ccf2795ba5998e |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T20:13:04Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-e8028793b1cb45edb9ccf2795ba5998e2023-11-24T00:08:34ZengMDPI AGAgronomy2073-43952022-03-0112375210.3390/agronomy12030752The Adenine at the 4th Exon of the <i>DRO1</i> Gene Provides Drought-Tolerance Capacity to Hybrid Rice Deyou4727 and Its Maintainer Line Dexiang074BFarhan Nabi0Sumbal Sajid1Guotao Yang2Abdul Rasheed Kaleri3Yating Zheng4Jian Qin5Xuechun Wang6Kaifeng Jiang7Youlin Peng8Yungao Hu9College of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaCollege of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaCollege of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaCollege of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaCollege of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaKey Laboratory of Southwest Rice Biology and Genetic Breeding, Ministry of Agriculture, Institute of Rice and Sorghum, Sichuan Academy of Agricultural Science, Deyang 618000, ChinaCollege of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaKey Laboratory of Southwest Rice Biology and Genetic Breeding, Ministry of Agriculture, Institute of Rice and Sorghum, Sichuan Academy of Agricultural Science, Deyang 618000, ChinaCollege of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaCollege of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaExtreme weather and global warming are drastic agricultural issues causing water scarcity. Under these conditions, it is vital to select drought-tolerant hybrid super rice breeds to enhance the rice yield in dry areas. In the current study, a dry-shed experiment was carried out in 2019–2020 to analyze the root growth, gene expression, and enzyme activity of hybrid rice Deyou4727, its parent (Dexiang074B * Chenghui727), taking IR64 as a universal control. Results showed that Deyou4727 and its maintainer line Dexiang074B produced more roots (30–50 cm soil) and higher enzymatic activity than IR64 during the drought stress period, indicating a good drought-tolerant capacity for Deyou4727. The distribution and morphology of the root system in Deyou4727 were inherited by the maintainer line Dexiang074B, which was confirmed by the high upregulation of <i>DRO1</i> and <i>OsPIN1b</i> gene expression and the sharing of the same ORF region in both the hybrid rice varieties. However, the <i>DRO1</i> gene ORF study showed that, in the case of the control IR64, ‘A’ was missing at the 4th exon of the <i>DRO1</i> gene, which may explain the shallow and weak root architecture system of IR64. The drought-tolerance capacity of Deyou4727 can be used in the future as a drought-resistant germplasm for breeding more drought-tolerant hybrid rice varieties.https://www.mdpi.com/2073-4395/12/3/752drought tolerance<i>DRO1</i>deep rootenzyme activitygermplasm |
| spellingShingle | Farhan Nabi Sumbal Sajid Guotao Yang Abdul Rasheed Kaleri Yating Zheng Jian Qin Xuechun Wang Kaifeng Jiang Youlin Peng Yungao Hu The Adenine at the 4th Exon of the <i>DRO1</i> Gene Provides Drought-Tolerance Capacity to Hybrid Rice Deyou4727 and Its Maintainer Line Dexiang074B Agronomy drought tolerance <i>DRO1</i> deep root enzyme activity germplasm |
| title | The Adenine at the 4th Exon of the <i>DRO1</i> Gene Provides Drought-Tolerance Capacity to Hybrid Rice Deyou4727 and Its Maintainer Line Dexiang074B |
| title_full | The Adenine at the 4th Exon of the <i>DRO1</i> Gene Provides Drought-Tolerance Capacity to Hybrid Rice Deyou4727 and Its Maintainer Line Dexiang074B |
| title_fullStr | The Adenine at the 4th Exon of the <i>DRO1</i> Gene Provides Drought-Tolerance Capacity to Hybrid Rice Deyou4727 and Its Maintainer Line Dexiang074B |
| title_full_unstemmed | The Adenine at the 4th Exon of the <i>DRO1</i> Gene Provides Drought-Tolerance Capacity to Hybrid Rice Deyou4727 and Its Maintainer Line Dexiang074B |
| title_short | The Adenine at the 4th Exon of the <i>DRO1</i> Gene Provides Drought-Tolerance Capacity to Hybrid Rice Deyou4727 and Its Maintainer Line Dexiang074B |
| title_sort | adenine at the 4th exon of the i dro1 i gene provides drought tolerance capacity to hybrid rice deyou4727 and its maintainer line dexiang074b |
| topic | drought tolerance <i>DRO1</i> deep root enzyme activity germplasm |
| url | https://www.mdpi.com/2073-4395/12/3/752 |
| work_keys_str_mv | AT farhannabi theadenineatthe4thexonoftheidro1igeneprovidesdroughttolerancecapacitytohybridricedeyou4727anditsmaintainerlinedexiang074b AT sumbalsajid theadenineatthe4thexonoftheidro1igeneprovidesdroughttolerancecapacitytohybridricedeyou4727anditsmaintainerlinedexiang074b AT guotaoyang theadenineatthe4thexonoftheidro1igeneprovidesdroughttolerancecapacitytohybridricedeyou4727anditsmaintainerlinedexiang074b AT abdulrasheedkaleri theadenineatthe4thexonoftheidro1igeneprovidesdroughttolerancecapacitytohybridricedeyou4727anditsmaintainerlinedexiang074b AT yatingzheng theadenineatthe4thexonoftheidro1igeneprovidesdroughttolerancecapacitytohybridricedeyou4727anditsmaintainerlinedexiang074b AT jianqin theadenineatthe4thexonoftheidro1igeneprovidesdroughttolerancecapacitytohybridricedeyou4727anditsmaintainerlinedexiang074b AT xuechunwang theadenineatthe4thexonoftheidro1igeneprovidesdroughttolerancecapacitytohybridricedeyou4727anditsmaintainerlinedexiang074b AT kaifengjiang theadenineatthe4thexonoftheidro1igeneprovidesdroughttolerancecapacitytohybridricedeyou4727anditsmaintainerlinedexiang074b AT youlinpeng theadenineatthe4thexonoftheidro1igeneprovidesdroughttolerancecapacitytohybridricedeyou4727anditsmaintainerlinedexiang074b AT yungaohu theadenineatthe4thexonoftheidro1igeneprovidesdroughttolerancecapacitytohybridricedeyou4727anditsmaintainerlinedexiang074b AT farhannabi adenineatthe4thexonoftheidro1igeneprovidesdroughttolerancecapacitytohybridricedeyou4727anditsmaintainerlinedexiang074b AT sumbalsajid adenineatthe4thexonoftheidro1igeneprovidesdroughttolerancecapacitytohybridricedeyou4727anditsmaintainerlinedexiang074b AT guotaoyang adenineatthe4thexonoftheidro1igeneprovidesdroughttolerancecapacitytohybridricedeyou4727anditsmaintainerlinedexiang074b AT abdulrasheedkaleri adenineatthe4thexonoftheidro1igeneprovidesdroughttolerancecapacitytohybridricedeyou4727anditsmaintainerlinedexiang074b AT yatingzheng adenineatthe4thexonoftheidro1igeneprovidesdroughttolerancecapacitytohybridricedeyou4727anditsmaintainerlinedexiang074b AT jianqin adenineatthe4thexonoftheidro1igeneprovidesdroughttolerancecapacitytohybridricedeyou4727anditsmaintainerlinedexiang074b AT xuechunwang adenineatthe4thexonoftheidro1igeneprovidesdroughttolerancecapacitytohybridricedeyou4727anditsmaintainerlinedexiang074b AT kaifengjiang adenineatthe4thexonoftheidro1igeneprovidesdroughttolerancecapacitytohybridricedeyou4727anditsmaintainerlinedexiang074b AT youlinpeng adenineatthe4thexonoftheidro1igeneprovidesdroughttolerancecapacitytohybridricedeyou4727anditsmaintainerlinedexiang074b AT yungaohu adenineatthe4thexonoftheidro1igeneprovidesdroughttolerancecapacitytohybridricedeyou4727anditsmaintainerlinedexiang074b |