Egg-adaptive mutations of human influenza H3N2 virus are contingent on natural evolution.
Egg-adaptive mutations in influenza hemagglutinin (HA) often emerge during the production of egg-based seasonal influenza vaccines, which contribute to the largest share in the global influenza vaccine market. While some egg-adaptive mutations have minimal impact on the HA antigenicity (e.g. G186V),...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-09-01
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| Series: | PLoS Pathogens |
| Online Access: | https://doi.org/10.1371/journal.ppat.1010875 |
| _version_ | 1811329301853765632 |
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| author | Weiwen Liang Timothy J C Tan Yiquan Wang Huibin Lv Yuanxin Sun Roberto Bruzzone Chris K P Mok Nicholas C Wu |
| author_facet | Weiwen Liang Timothy J C Tan Yiquan Wang Huibin Lv Yuanxin Sun Roberto Bruzzone Chris K P Mok Nicholas C Wu |
| author_sort | Weiwen Liang |
| collection | DOAJ |
| description | Egg-adaptive mutations in influenza hemagglutinin (HA) often emerge during the production of egg-based seasonal influenza vaccines, which contribute to the largest share in the global influenza vaccine market. While some egg-adaptive mutations have minimal impact on the HA antigenicity (e.g. G186V), others can alter it (e.g. L194P). Here, we show that the preference of egg-adaptive mutation in human H3N2 HA is strain-dependent. In particular, Thr160 and Asn190, which are found in many recent H3N2 strains, restrict the emergence of L194P but not G186V. Our results further suggest that natural amino acid variants at other HA residues also play a role in determining the preference of egg-adaptive mutation. Consistently, recent human H3N2 strains from different clades acquire different mutations during egg passaging. Overall, these results demonstrate that natural mutations in human H3N2 HA can influence the preference of egg-adaptation mutation, which has important implications in seed strain selection for egg-based influenza vaccine. |
| first_indexed | 2024-04-13T15:41:24Z |
| format | Article |
| id | doaj.art-e80291d5971a405e81800a812414806f |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-04-13T15:41:24Z |
| publishDate | 2022-09-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-e80291d5971a405e81800a812414806f2022-12-22T02:41:08ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742022-09-01189e101087510.1371/journal.ppat.1010875Egg-adaptive mutations of human influenza H3N2 virus are contingent on natural evolution.Weiwen LiangTimothy J C TanYiquan WangHuibin LvYuanxin SunRoberto BruzzoneChris K P MokNicholas C WuEgg-adaptive mutations in influenza hemagglutinin (HA) often emerge during the production of egg-based seasonal influenza vaccines, which contribute to the largest share in the global influenza vaccine market. While some egg-adaptive mutations have minimal impact on the HA antigenicity (e.g. G186V), others can alter it (e.g. L194P). Here, we show that the preference of egg-adaptive mutation in human H3N2 HA is strain-dependent. In particular, Thr160 and Asn190, which are found in many recent H3N2 strains, restrict the emergence of L194P but not G186V. Our results further suggest that natural amino acid variants at other HA residues also play a role in determining the preference of egg-adaptive mutation. Consistently, recent human H3N2 strains from different clades acquire different mutations during egg passaging. Overall, these results demonstrate that natural mutations in human H3N2 HA can influence the preference of egg-adaptation mutation, which has important implications in seed strain selection for egg-based influenza vaccine.https://doi.org/10.1371/journal.ppat.1010875 |
| spellingShingle | Weiwen Liang Timothy J C Tan Yiquan Wang Huibin Lv Yuanxin Sun Roberto Bruzzone Chris K P Mok Nicholas C Wu Egg-adaptive mutations of human influenza H3N2 virus are contingent on natural evolution. PLoS Pathogens |
| title | Egg-adaptive mutations of human influenza H3N2 virus are contingent on natural evolution. |
| title_full | Egg-adaptive mutations of human influenza H3N2 virus are contingent on natural evolution. |
| title_fullStr | Egg-adaptive mutations of human influenza H3N2 virus are contingent on natural evolution. |
| title_full_unstemmed | Egg-adaptive mutations of human influenza H3N2 virus are contingent on natural evolution. |
| title_short | Egg-adaptive mutations of human influenza H3N2 virus are contingent on natural evolution. |
| title_sort | egg adaptive mutations of human influenza h3n2 virus are contingent on natural evolution |
| url | https://doi.org/10.1371/journal.ppat.1010875 |
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