Effect of β-agonist on the dexamethasone-induced expression of aromatase by the human monocyte cells
Emerging evidence suggests that sex steroids are important for human skin health. In particular, estrogen improves skin thickness, elasticity and moisture of older women. The major source of circulating estrogen is the ovary; however, local estrogen synthesis and secretion have important roles in,...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Bioscientifica
2017-02-01
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| Series: | Endocrine Connections |
| Subjects: | |
| Online Access: | http://www.endocrineconnections.com/content/6/2/82.full |
| _version_ | 1811284458252271616 |
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| author | Masatada Watanabe Shuji Ohno Hiroshi Wachi |
| author_facet | Masatada Watanabe Shuji Ohno Hiroshi Wachi |
| author_sort | Masatada Watanabe |
| collection | DOAJ |
| description | Emerging evidence suggests that sex steroids are important for human skin health. In particular, estrogen improves skin thickness, elasticity and moisture of older women.
The major source of circulating estrogen is the ovary; however, local estrogen synthesis and secretion have important roles in, for example, bone metabolism and breast cancer development. We hypothesized that infiltrated peripheral monocytes are one of the
sources of estrogen in skin tissues. We also hypothesized that, during atopic dermatitis under stress, a decline in the hypothalamus–pituitary–adrenal axis (HPA) and facilitation
of the (hypothalamus)–sympathetic–adrenomedullary system (SAM) attenuates estrogen secretion from monocytes. Based on this hypothesis, we tested aromatase expression in the human peripheral monocyte-derived cell line THP-1 in response to the synthetic glucocorticoid dexamethasone (Dex), the synthetic β-agonist isoproterenol (Iso) and the β-antagonist propranolol (Pro). Dex mimics glucocorticoid secreted during excitation
of the HPA, and Iso mimics catecholamine secreted during excitation of the SAM. We found that aromatase activity and the CYP19A1 gene transcript were both upregulated in THP-1 cells in the presence of Dex. Addition of Iso induced their downregulation
and further addition of Pro rescued aromatase expression. These results may suggest that attenuation of estrogen secretion from peripheral monocytes could be a part of the pathology of stress-caused deterioration of atopic dermatitis. Further examination
using an in vitro human skin model including THP-1 cells might be a valuable tool for investigating the therapeutic efficacy and mechanism of estrogen treatment for skin health. |
| first_indexed | 2024-04-13T02:28:55Z |
| format | Article |
| id | doaj.art-e808246f68df4173ba8f4f266a1a41fb |
| institution | Directory Open Access Journal |
| issn | 2049-3614 2049-3614 |
| language | English |
| last_indexed | 2024-04-13T02:28:55Z |
| publishDate | 2017-02-01 |
| publisher | Bioscientifica |
| record_format | Article |
| series | Endocrine Connections |
| spelling | doaj.art-e808246f68df4173ba8f4f266a1a41fb2022-12-22T03:06:40ZengBioscientificaEndocrine Connections2049-36142049-36142017-02-0162828810.1530/EC-16-0099Effect of β-agonist on the dexamethasone-induced expression of aromatase by the human monocyte cellsMasatada Watanabe0Shuji Ohno1Hiroshi Wachi2Laboratory of Tissue Regeneration, Hoshi University School of Pharmacy and Pharmaceutical Sciences, Shinagawa, Tokyo, JapanDivision of Research for Pharmacy Students Education, Hoshi University, Shinagawa, Tokyo, JapanLaboratory of Tissue Regeneration, Hoshi University School of Pharmacy and Pharmaceutical Sciences, Shinagawa, Tokyo, JapanEmerging evidence suggests that sex steroids are important for human skin health. In particular, estrogen improves skin thickness, elasticity and moisture of older women. The major source of circulating estrogen is the ovary; however, local estrogen synthesis and secretion have important roles in, for example, bone metabolism and breast cancer development. We hypothesized that infiltrated peripheral monocytes are one of the sources of estrogen in skin tissues. We also hypothesized that, during atopic dermatitis under stress, a decline in the hypothalamus–pituitary–adrenal axis (HPA) and facilitation of the (hypothalamus)–sympathetic–adrenomedullary system (SAM) attenuates estrogen secretion from monocytes. Based on this hypothesis, we tested aromatase expression in the human peripheral monocyte-derived cell line THP-1 in response to the synthetic glucocorticoid dexamethasone (Dex), the synthetic β-agonist isoproterenol (Iso) and the β-antagonist propranolol (Pro). Dex mimics glucocorticoid secreted during excitation of the HPA, and Iso mimics catecholamine secreted during excitation of the SAM. We found that aromatase activity and the CYP19A1 gene transcript were both upregulated in THP-1 cells in the presence of Dex. Addition of Iso induced their downregulation and further addition of Pro rescued aromatase expression. These results may suggest that attenuation of estrogen secretion from peripheral monocytes could be a part of the pathology of stress-caused deterioration of atopic dermatitis. Further examination using an in vitro human skin model including THP-1 cells might be a valuable tool for investigating the therapeutic efficacy and mechanism of estrogen treatment for skin health.http://www.endocrineconnections.com/content/6/2/82.fullβ-agonistaromataseatopic dermatitismonocyteestrogen |
| spellingShingle | Masatada Watanabe Shuji Ohno Hiroshi Wachi Effect of β-agonist on the dexamethasone-induced expression of aromatase by the human monocyte cells Endocrine Connections β-agonist aromatase atopic dermatitis monocyte estrogen |
| title | Effect of β-agonist on the dexamethasone-induced expression of aromatase by the human monocyte cells |
| title_full | Effect of β-agonist on the dexamethasone-induced expression of aromatase by the human monocyte cells |
| title_fullStr | Effect of β-agonist on the dexamethasone-induced expression of aromatase by the human monocyte cells |
| title_full_unstemmed | Effect of β-agonist on the dexamethasone-induced expression of aromatase by the human monocyte cells |
| title_short | Effect of β-agonist on the dexamethasone-induced expression of aromatase by the human monocyte cells |
| title_sort | effect of β agonist on the dexamethasone induced expression of aromatase by the human monocyte cells |
| topic | β-agonist aromatase atopic dermatitis monocyte estrogen |
| url | http://www.endocrineconnections.com/content/6/2/82.full |
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