Effect of salicylic acid on callus formation in three <i>Theobroma cacao</i> L. clones

Cocoa is a crop of great economic importance and it has interest as a source of naturally occurring compounds. The tissue culture using metabolic elicitation is an alternative for large scale production of secondary metabolites. This study aimed to determine the effect of salicylic acid as elicitor...

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Main Authors: Lillien Fajardo Rosabal, Juan J. Silva Pupo, Yosvel Viera Tamayo, Yoeldis Cobas Rodríguez
Format: Article
Language:English
Published: Universidad Central Marta Abreu de Las Villas 2015-10-01
Series:Biotecnología Vegetal
Online Access:https://revista.ibp.co.cu/index.php/BV/article/view/499
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author Lillien Fajardo Rosabal
Juan J. Silva Pupo
Yosvel Viera Tamayo
Yoeldis Cobas Rodríguez
author_facet Lillien Fajardo Rosabal
Juan J. Silva Pupo
Yosvel Viera Tamayo
Yoeldis Cobas Rodríguez
author_sort Lillien Fajardo Rosabal
collection DOAJ
description Cocoa is a crop of great economic importance and it has interest as a source of naturally occurring compounds. The tissue culture using metabolic elicitation is an alternative for large scale production of secondary metabolites. This study aimed to determine the effect of salicylic acid as elicitor for the production of cell biomass of Theobroma cacao L. in three clones. For callus formation staminodes of ‘UF-613’, ‘UF-650’ and ‘Pound-7’ clones were used. It were added 0.02, 0.05, 0.10 and 0.15 mg l-1 salicylic acid to the culture medium. At 15 and 28 days of culture the number of explants formed callus were quantified and the percentage of callus formation was calculated. In the best results clone the secondary metabolites in callus and culture medium were identified by phytochemical screening. The addition of salicylic acid accelerated callus formation in the cocoa clones ‘UF-613’, ‘UF-650’ and ‘Pound-7’. The results depended of the clone and the concentrations used. The identification of secondary metabolites in callus of ‘UF-650’ clone and in the culture medium is the starting point for future research.   Key words: tissue culture, phytochemical screening, secondary metabolites.
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spelling doaj.art-e808aa55c4bc4df9908c2654ae8d30b32022-12-22T00:33:41ZengUniversidad Central Marta Abreu de Las VillasBiotecnología Vegetal1609-18412074-86472015-10-01154459Effect of salicylic acid on callus formation in three <i>Theobroma cacao</i> L. clonesLillien Fajardo RosabalJuan J. Silva PupoYosvel Viera TamayoYoeldis Cobas RodríguezCocoa is a crop of great economic importance and it has interest as a source of naturally occurring compounds. The tissue culture using metabolic elicitation is an alternative for large scale production of secondary metabolites. This study aimed to determine the effect of salicylic acid as elicitor for the production of cell biomass of Theobroma cacao L. in three clones. For callus formation staminodes of ‘UF-613’, ‘UF-650’ and ‘Pound-7’ clones were used. It were added 0.02, 0.05, 0.10 and 0.15 mg l-1 salicylic acid to the culture medium. At 15 and 28 days of culture the number of explants formed callus were quantified and the percentage of callus formation was calculated. In the best results clone the secondary metabolites in callus and culture medium were identified by phytochemical screening. The addition of salicylic acid accelerated callus formation in the cocoa clones ‘UF-613’, ‘UF-650’ and ‘Pound-7’. The results depended of the clone and the concentrations used. The identification of secondary metabolites in callus of ‘UF-650’ clone and in the culture medium is the starting point for future research.   Key words: tissue culture, phytochemical screening, secondary metabolites.https://revista.ibp.co.cu/index.php/BV/article/view/499
spellingShingle Lillien Fajardo Rosabal
Juan J. Silva Pupo
Yosvel Viera Tamayo
Yoeldis Cobas Rodríguez
Effect of salicylic acid on callus formation in three <i>Theobroma cacao</i> L. clones
Biotecnología Vegetal
title Effect of salicylic acid on callus formation in three <i>Theobroma cacao</i> L. clones
title_full Effect of salicylic acid on callus formation in three <i>Theobroma cacao</i> L. clones
title_fullStr Effect of salicylic acid on callus formation in three <i>Theobroma cacao</i> L. clones
title_full_unstemmed Effect of salicylic acid on callus formation in three <i>Theobroma cacao</i> L. clones
title_short Effect of salicylic acid on callus formation in three <i>Theobroma cacao</i> L. clones
title_sort effect of salicylic acid on callus formation in three i theobroma cacao i l clones
url https://revista.ibp.co.cu/index.php/BV/article/view/499
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