Differential Glycosylation Levels in Saliva from Patients with Lung or Breast Cancer: A Preliminary Assessment for Early Diagnostic Purposes
Glycans play a fundamental role in several biological processes, such as cell–cell adhesion, signaling, and recognition. Similarly, abnormal glycosylation is involved in many pathological processes, among which include tumor growth and progression. Several highly glycosylated proteins found in blood...
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MDPI AG
2021-08-01
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| Online Access: | https://www.mdpi.com/2218-1989/11/9/566 |
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| author | Andrea Ragusa Pietrina Romano Marcello Salvatore Lenucci Emanuela Civino Daniele Vergara Elena Pitotti Cosimo Neglia Alessandro Distante Giampiero Diego Romano Nicola Di Renzo Giammarco Surico Prisco Piscitelli Michele Maffia |
| author_facet | Andrea Ragusa Pietrina Romano Marcello Salvatore Lenucci Emanuela Civino Daniele Vergara Elena Pitotti Cosimo Neglia Alessandro Distante Giampiero Diego Romano Nicola Di Renzo Giammarco Surico Prisco Piscitelli Michele Maffia |
| author_sort | Andrea Ragusa |
| collection | DOAJ |
| description | Glycans play a fundamental role in several biological processes, such as cell–cell adhesion, signaling, and recognition. Similarly, abnormal glycosylation is involved in many pathological processes, among which include tumor growth and progression. Several highly glycosylated proteins found in blood are currently used in clinical practice as cancer biomarkers (e.g., CA125, PSA, and CA19-9). The development of novel non-invasive diagnostic procedures would greatly simplify the screening and discovery of pathologies at an early stage, thus also allowing for simpler treatment and a higher success rate. In this observational study carried out on 68 subjects diagnosed with either breast or lung cancer and 34 healthy volunteers, we hydrolyzed the glycoproteins in saliva and quantified the obtained free sugars (fucose, mannose, galactose, glucosamine, and galactosamine) by using high-performance anion-exchange chromatography with pulsed-amperometric detection (HPAEC-PAD). The glycosidic profiles were compared by using multivariate statistical analysis, showing differential glycosylation patterns among the three categories. Furthermore, Receiver Operating Characteristics (ROC) analysis allowed obtaining a reliable and minimally invasive protocol able to discriminate between healthy and pathological subjects. |
| first_indexed | 2024-03-10T07:27:31Z |
| format | Article |
| id | doaj.art-e80b1748ffcb4e6f9566366722483936 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-10T07:27:31Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-e80b1748ffcb4e6f95663667224839362023-11-22T14:11:01ZengMDPI AGMetabolites2218-19892021-08-0111956610.3390/metabo11090566Differential Glycosylation Levels in Saliva from Patients with Lung or Breast Cancer: A Preliminary Assessment for Early Diagnostic PurposesAndrea Ragusa0Pietrina Romano1Marcello Salvatore Lenucci2Emanuela Civino3Daniele Vergara4Elena Pitotti5Cosimo Neglia6Alessandro Distante7Giampiero Diego Romano8Nicola Di Renzo9Giammarco Surico10Prisco Piscitelli11Michele Maffia12Department of Biological and Environmental Sciences and Technologies, Campus Ecotekne, University of Salento, via Monteroni, 73100 Lecce, ItalyMedical Oncology Unit, “Vito Fazzi” Hospital, 73100 Lecce, ItalyDepartment of Biological and Environmental Sciences and Technologies, Campus Ecotekne, University of Salento, via Monteroni, 73100 Lecce, ItalyDepartment of Biological and Environmental Sciences and Technologies, Campus Ecotekne, University of Salento, via Monteroni, 73100 Lecce, ItalyDepartment of Biological and Environmental Sciences and Technologies, Campus Ecotekne, University of Salento, via Monteroni, 73100 Lecce, ItalyLocal Health Authority ASL Lecce, 73100 Lecce, ItalyEuro Mediterranean Scientific Biomedical Institute (ISBEM), 72100 Brindisi, ItalyEuro Mediterranean Scientific Biomedical Institute (ISBEM), 72100 Brindisi, ItalyMedical Oncology Unit, “Vito Fazzi” Hospital, 73100 Lecce, ItalyMedical Oncology Unit, “Vito Fazzi” Hospital, 73100 Lecce, ItalyMedical Oncology Unit, “Vito Fazzi” Hospital, 73100 Lecce, ItalyLocal Health Authority ASL Lecce, 73100 Lecce, ItalyDepartment of Biological and Environmental Sciences and Technologies, Campus Ecotekne, University of Salento, via Monteroni, 73100 Lecce, ItalyGlycans play a fundamental role in several biological processes, such as cell–cell adhesion, signaling, and recognition. Similarly, abnormal glycosylation is involved in many pathological processes, among which include tumor growth and progression. Several highly glycosylated proteins found in blood are currently used in clinical practice as cancer biomarkers (e.g., CA125, PSA, and CA19-9). The development of novel non-invasive diagnostic procedures would greatly simplify the screening and discovery of pathologies at an early stage, thus also allowing for simpler treatment and a higher success rate. In this observational study carried out on 68 subjects diagnosed with either breast or lung cancer and 34 healthy volunteers, we hydrolyzed the glycoproteins in saliva and quantified the obtained free sugars (fucose, mannose, galactose, glucosamine, and galactosamine) by using high-performance anion-exchange chromatography with pulsed-amperometric detection (HPAEC-PAD). The glycosidic profiles were compared by using multivariate statistical analysis, showing differential glycosylation patterns among the three categories. Furthermore, Receiver Operating Characteristics (ROC) analysis allowed obtaining a reliable and minimally invasive protocol able to discriminate between healthy and pathological subjects.https://www.mdpi.com/2218-1989/11/9/566HPEAC-PADglycoproteinglycomicsglycosylationsalivaearly diagnosis |
| spellingShingle | Andrea Ragusa Pietrina Romano Marcello Salvatore Lenucci Emanuela Civino Daniele Vergara Elena Pitotti Cosimo Neglia Alessandro Distante Giampiero Diego Romano Nicola Di Renzo Giammarco Surico Prisco Piscitelli Michele Maffia Differential Glycosylation Levels in Saliva from Patients with Lung or Breast Cancer: A Preliminary Assessment for Early Diagnostic Purposes Metabolites HPEAC-PAD glycoprotein glycomics glycosylation saliva early diagnosis |
| title | Differential Glycosylation Levels in Saliva from Patients with Lung or Breast Cancer: A Preliminary Assessment for Early Diagnostic Purposes |
| title_full | Differential Glycosylation Levels in Saliva from Patients with Lung or Breast Cancer: A Preliminary Assessment for Early Diagnostic Purposes |
| title_fullStr | Differential Glycosylation Levels in Saliva from Patients with Lung or Breast Cancer: A Preliminary Assessment for Early Diagnostic Purposes |
| title_full_unstemmed | Differential Glycosylation Levels in Saliva from Patients with Lung or Breast Cancer: A Preliminary Assessment for Early Diagnostic Purposes |
| title_short | Differential Glycosylation Levels in Saliva from Patients with Lung or Breast Cancer: A Preliminary Assessment for Early Diagnostic Purposes |
| title_sort | differential glycosylation levels in saliva from patients with lung or breast cancer a preliminary assessment for early diagnostic purposes |
| topic | HPEAC-PAD glycoprotein glycomics glycosylation saliva early diagnosis |
| url | https://www.mdpi.com/2218-1989/11/9/566 |
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