In Vivo Anchoring Bis‐Pyrene Probe for Molecular Imaging of Early Gastric Cancer by Endoscopic Techniques
Abstract With the development of blue laser endoscopy (BLE) technique, it's often used to diagnose early gastric cancer (EGC) by the morphological changes of blood vessels through BLE. However, EGC is still not obvious to identify, resulting in a high rate of missed diagnosis. Molecular imaging...
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| Format: | Article |
| Language: | English |
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Wiley
2023-02-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202203918 |
| _version_ | 1797688126393548800 |
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| author | Qiang Luo Chaoqiang Fan Wang Ying Xue Peng Yiyang Hu Zhaohui Luan Shaosong Ye Chunli Gong Yu Huang Yufeng Xiao Yang Chen Malcolm Xing Lei Wang Shiming Yang |
| author_facet | Qiang Luo Chaoqiang Fan Wang Ying Xue Peng Yiyang Hu Zhaohui Luan Shaosong Ye Chunli Gong Yu Huang Yufeng Xiao Yang Chen Malcolm Xing Lei Wang Shiming Yang |
| author_sort | Qiang Luo |
| collection | DOAJ |
| description | Abstract With the development of blue laser endoscopy (BLE) technique, it's often used to diagnose early gastric cancer (EGC) by the morphological changes of blood vessels through BLE. However, EGC is still not obvious to identify, resulting in a high rate of missed diagnosis. Molecular imaging can show the changes in early tumors at molecular level, which provides a possibility for diagnosing EGC. Therefore, developing a probe that visually monitors blood vessels of EGC under BLE is particularly necessary. Herein, a bis‐pyrene (BP) based nanoprobe (BP‐FFVLK‐(PEG)‐RGD, M1) is designed, which can target angiogenesis and self‐assemble into fibers in situ, resulting in stable and long‐term retention in tumor. Moreover, M1 probe can emit yellow‐green fluorescence for imaging under BLE. M1 probe is confirmed to steadily remain in tumor for up to 96 hours in mice transplanted subcutaneously. In addition, the M1 probe is able to target angiogenesis for molecular imaging of isolated human gastric cancer tissue under BLE. Finally, M1 probe i.v. injected into primary gastric cancer model rabbits successfully highlighted the tumor site under BLE, which is confirmed by pathological analysis. It's the first time to develop a probe for diagnosing EGC by visualizing angiogenesis under BLE, showing great clinical significance. |
| first_indexed | 2024-03-12T01:27:50Z |
| format | Article |
| id | doaj.art-e8104d4405b9403f9bf66223d886f90a |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-03-12T01:27:50Z |
| publishDate | 2023-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-e8104d4405b9403f9bf66223d886f90a2023-09-12T14:40:47ZengWileyAdvanced Science2198-38442023-02-01104n/an/a10.1002/advs.202203918In Vivo Anchoring Bis‐Pyrene Probe for Molecular Imaging of Early Gastric Cancer by Endoscopic TechniquesQiang Luo0Chaoqiang Fan1Wang Ying2Xue Peng3Yiyang Hu4Zhaohui Luan5Shaosong Ye6Chunli Gong7Yu Huang8Yufeng Xiao9Yang Chen10Malcolm Xing11Lei Wang12Shiming Yang13Department of Gastroenterology Xinqiao Hospital Army Medical University Chongqing City 400037 P. R. ChinaDepartment of Gastroenterology Xinqiao Hospital Army Medical University Chongqing City 400037 P. R. ChinaDepartment of Gastroenterology Xinqiao Hospital Army Medical University Chongqing City 400037 P. R. ChinaDepartment of Gastroenterology Xinqiao Hospital Army Medical University Chongqing City 400037 P. R. ChinaDepartment of Gastroenterology Xinqiao Hospital Army Medical University Chongqing City 400037 P. R. ChinaDepartment of Gastroenterology Xinqiao Hospital Army Medical University Chongqing City 400037 P. R. ChinaDepartment of Gastroenterology Xinqiao Hospital Army Medical University Chongqing City 400037 P. R. ChinaDepartment of Gastroenterology Xinqiao Hospital Army Medical University Chongqing City 400037 P. R. ChinaDepartment of Gastroenterology Xinqiao Hospital Army Medical University Chongqing City 400037 P. R. ChinaDepartment of Gastroenterology Xinqiao Hospital Army Medical University Chongqing City 400037 P. R. ChinaDepartment of Gastroenterology Xinqiao Hospital Army Medical University Chongqing City 400037 P. R. ChinaDepartment of Mechanical Engineering, Biochemistry and Medical Genetics University of Manitoba Winnipeg Manitoba R3T 2N2 CanadaCAS Center for Excellence in Nanoscience CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety National Center for Nanoscience and Technology (NCNST) No. 11 Beiyitiao, Zhongguancun Beijing 100190 ChinaDepartment of Gastroenterology Xinqiao Hospital Army Medical University Chongqing City 400037 P. R. ChinaAbstract With the development of blue laser endoscopy (BLE) technique, it's often used to diagnose early gastric cancer (EGC) by the morphological changes of blood vessels through BLE. However, EGC is still not obvious to identify, resulting in a high rate of missed diagnosis. Molecular imaging can show the changes in early tumors at molecular level, which provides a possibility for diagnosing EGC. Therefore, developing a probe that visually monitors blood vessels of EGC under BLE is particularly necessary. Herein, a bis‐pyrene (BP) based nanoprobe (BP‐FFVLK‐(PEG)‐RGD, M1) is designed, which can target angiogenesis and self‐assemble into fibers in situ, resulting in stable and long‐term retention in tumor. Moreover, M1 probe can emit yellow‐green fluorescence for imaging under BLE. M1 probe is confirmed to steadily remain in tumor for up to 96 hours in mice transplanted subcutaneously. In addition, the M1 probe is able to target angiogenesis for molecular imaging of isolated human gastric cancer tissue under BLE. Finally, M1 probe i.v. injected into primary gastric cancer model rabbits successfully highlighted the tumor site under BLE, which is confirmed by pathological analysis. It's the first time to develop a probe for diagnosing EGC by visualizing angiogenesis under BLE, showing great clinical significance.https://doi.org/10.1002/advs.202203918blue laser endoscopyearly gastric cancermolecular imagingangiogenesis |
| spellingShingle | Qiang Luo Chaoqiang Fan Wang Ying Xue Peng Yiyang Hu Zhaohui Luan Shaosong Ye Chunli Gong Yu Huang Yufeng Xiao Yang Chen Malcolm Xing Lei Wang Shiming Yang In Vivo Anchoring Bis‐Pyrene Probe for Molecular Imaging of Early Gastric Cancer by Endoscopic Techniques Advanced Science blue laser endoscopy early gastric cancer molecular imaging angiogenesis |
| title | In Vivo Anchoring Bis‐Pyrene Probe for Molecular Imaging of Early Gastric Cancer by Endoscopic Techniques |
| title_full | In Vivo Anchoring Bis‐Pyrene Probe for Molecular Imaging of Early Gastric Cancer by Endoscopic Techniques |
| title_fullStr | In Vivo Anchoring Bis‐Pyrene Probe for Molecular Imaging of Early Gastric Cancer by Endoscopic Techniques |
| title_full_unstemmed | In Vivo Anchoring Bis‐Pyrene Probe for Molecular Imaging of Early Gastric Cancer by Endoscopic Techniques |
| title_short | In Vivo Anchoring Bis‐Pyrene Probe for Molecular Imaging of Early Gastric Cancer by Endoscopic Techniques |
| title_sort | in vivo anchoring bis pyrene probe for molecular imaging of early gastric cancer by endoscopic techniques |
| topic | blue laser endoscopy early gastric cancer molecular imaging angiogenesis |
| url | https://doi.org/10.1002/advs.202203918 |
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