Prevalence of Escherichia coli isolated from oropharynx and trachea of clinically sick poultry and antimicrobial resistance pattern of the strains isolated
The study was carried out to investigate the isolation of Escherichia coli from tracheal and oropharyngeal swab of clinically sick chickens. The antibiotic susceptibility patterns of the isolates to several antimicrobials were determined with a striking emphasis on oxytetracycline. The PCR technique...
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Elsevier
2023-12-01
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| Series: | Biochemistry and Biophysics Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S240558082300136X |
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| author | Tanima Ferdous Shaolin Ferdouse Md. Shahadat Hossain M. Sohidullah Yaing Nue Frue Marma Sabuj Kanti Nath Paritosh Kumar Biswas |
| author_facet | Tanima Ferdous Shaolin Ferdouse Md. Shahadat Hossain M. Sohidullah Yaing Nue Frue Marma Sabuj Kanti Nath Paritosh Kumar Biswas |
| author_sort | Tanima Ferdous |
| collection | DOAJ |
| description | The study was carried out to investigate the isolation of Escherichia coli from tracheal and oropharyngeal swab of clinically sick chickens. The antibiotic susceptibility patterns of the isolates to several antimicrobials were determined with a striking emphasis on oxytetracycline. The PCR technique was applied to detect tetA, tetB, and tetC in the tetracycline-resistant isolates. The isolates were initially screened for their resistance patterns against 6 antimicrobials of six different groups using the disc diffusion technique. The results showed that 41% tracheal, 51% oropharyngeal, and 34% samples from both sites were E. coli positive respectively. Antimicrobial resistance profiling of the isolates revealed that all the isolates were resistant to oxytetracycline and sulphamethoxazole-trimethoprim, and also 90 %, 82.9%, 63.4%, and 39% resistant to ciprofloxacin, amoxicillin, gentamicin, and colistin respectively. Notably, 82.9% isolates (95% CI 68.4%–91.8%) showed resistance to ≥3 groups of antimicrobials that means these were multi-drug resistant. Among the tetracycline-resistant isolates, 85.4% (95% CI 71.2%–93.5%), 29.3% (7.5%–44.6%), and 7.3% (1.8% - 20.1) were positive for tetA, tetB, and tetC genes respectively. The frequency of the isolation of E. coli is greater in oropharyngeal than tracheal and both kinds of samples. Commercial poultry with E. coli strains has acquired extensive resistance to oxytetracycline. This study suggests a possible association between the tetA gene and oxytetracycline resistance in E. coli isolates, but further investigations like knockdown, whole-genome sequencing, and rescue experiments are needed to establish a direct causal relationship. |
| first_indexed | 2024-03-09T07:34:44Z |
| format | Article |
| id | doaj.art-e810955679fe4a4fa1bd02dcb46c5acb |
| institution | Directory Open Access Journal |
| issn | 2405-5808 |
| language | English |
| last_indexed | 2024-03-09T07:34:44Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj.art-e810955679fe4a4fa1bd02dcb46c5acb2023-12-03T05:41:53ZengElsevierBiochemistry and Biophysics Reports2405-58082023-12-0136101555Prevalence of Escherichia coli isolated from oropharynx and trachea of clinically sick poultry and antimicrobial resistance pattern of the strains isolatedTanima Ferdous0Shaolin Ferdouse1Md. Shahadat Hossain2M. Sohidullah3Yaing Nue Frue Marma4Sabuj Kanti Nath5Paritosh Kumar Biswas6Department of Microbiology and Veterinary Public Health, Faculty of Veterinary Medicine, Chattogram Veterinary and Animal Sciences University, Khulshi, 4225, Chattogram, BangladeshDepartment of Animal Nutrition, Faculty of Veterinary, Animal and Biomedical Sciences, Khulna Agricultural University, Khulna, 9100, BangladeshUpazilla Livestock Office and Veterinary Hospital, Dighalia, 9220, Khulna, BangladeshDepartment of Microbiology and Public Health, Faculty of Veterinary, Animal and Biomedical Sciences, Khulna Agricultural University, Khulna, 9100, BangladeshLivestock Extension Officer, Upazilla Livestock Office and Veterinary Hospital, Lohagara, 4396, Chattogram, BangladeshDepartment of Animal Nutrition, Faculty of Veterinary, Animal and Biomedical Sciences, Khulna Agricultural University, Khulna, 9100, Bangladesh; Corresponding author.Department of Microbiology and Veterinary Public Health, Faculty of Veterinary Medicine, Chattogram Veterinary and Animal Sciences University, Khulshi, 4225, Chattogram, BangladeshThe study was carried out to investigate the isolation of Escherichia coli from tracheal and oropharyngeal swab of clinically sick chickens. The antibiotic susceptibility patterns of the isolates to several antimicrobials were determined with a striking emphasis on oxytetracycline. The PCR technique was applied to detect tetA, tetB, and tetC in the tetracycline-resistant isolates. The isolates were initially screened for their resistance patterns against 6 antimicrobials of six different groups using the disc diffusion technique. The results showed that 41% tracheal, 51% oropharyngeal, and 34% samples from both sites were E. coli positive respectively. Antimicrobial resistance profiling of the isolates revealed that all the isolates were resistant to oxytetracycline and sulphamethoxazole-trimethoprim, and also 90 %, 82.9%, 63.4%, and 39% resistant to ciprofloxacin, amoxicillin, gentamicin, and colistin respectively. Notably, 82.9% isolates (95% CI 68.4%–91.8%) showed resistance to ≥3 groups of antimicrobials that means these were multi-drug resistant. Among the tetracycline-resistant isolates, 85.4% (95% CI 71.2%–93.5%), 29.3% (7.5%–44.6%), and 7.3% (1.8% - 20.1) were positive for tetA, tetB, and tetC genes respectively. The frequency of the isolation of E. coli is greater in oropharyngeal than tracheal and both kinds of samples. Commercial poultry with E. coli strains has acquired extensive resistance to oxytetracycline. This study suggests a possible association between the tetA gene and oxytetracycline resistance in E. coli isolates, but further investigations like knockdown, whole-genome sequencing, and rescue experiments are needed to establish a direct causal relationship.http://www.sciencedirect.com/science/article/pii/S240558082300136XAntimicrobialsOxytetracyclinePCRGenesAntibiotics |
| spellingShingle | Tanima Ferdous Shaolin Ferdouse Md. Shahadat Hossain M. Sohidullah Yaing Nue Frue Marma Sabuj Kanti Nath Paritosh Kumar Biswas Prevalence of Escherichia coli isolated from oropharynx and trachea of clinically sick poultry and antimicrobial resistance pattern of the strains isolated Biochemistry and Biophysics Reports Antimicrobials Oxytetracycline PCR Genes Antibiotics |
| title | Prevalence of Escherichia coli isolated from oropharynx and trachea of clinically sick poultry and antimicrobial resistance pattern of the strains isolated |
| title_full | Prevalence of Escherichia coli isolated from oropharynx and trachea of clinically sick poultry and antimicrobial resistance pattern of the strains isolated |
| title_fullStr | Prevalence of Escherichia coli isolated from oropharynx and trachea of clinically sick poultry and antimicrobial resistance pattern of the strains isolated |
| title_full_unstemmed | Prevalence of Escherichia coli isolated from oropharynx and trachea of clinically sick poultry and antimicrobial resistance pattern of the strains isolated |
| title_short | Prevalence of Escherichia coli isolated from oropharynx and trachea of clinically sick poultry and antimicrobial resistance pattern of the strains isolated |
| title_sort | prevalence of escherichia coli isolated from oropharynx and trachea of clinically sick poultry and antimicrobial resistance pattern of the strains isolated |
| topic | Antimicrobials Oxytetracycline PCR Genes Antibiotics |
| url | http://www.sciencedirect.com/science/article/pii/S240558082300136X |
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