PEPTIDES WITH ANTIMICROBIAL ACTIVITY PRODUCED BY ISOLATED NATIVE MICROORGANISMS
Like proteins, antimicrobial peptides (AMP) are versatile molecules synthesized by microorganisms using enzymatic pathways with no genetic code instruction. AMP have interesting properties in the food and pharmaceutical industries, like their antimicrobial ability against pathogens. Looking for biom...
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| Format: | Article |
| Language: | English |
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Universidad de Antioquia
2010-07-01
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| Series: | Vitae |
| Subjects: | |
| Online Access: | https://revistas.udea.edu.co/index.php/vitae/article/view/6343 |
| _version_ | 1828956034869231616 |
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| author | Ligia L. CORRALES-GARCÍA Gelmy L. CIRO G. |
| author_facet | Ligia L. CORRALES-GARCÍA Gelmy L. CIRO G. |
| author_sort | Ligia L. CORRALES-GARCÍA |
| collection | DOAJ |
| description | Like proteins, antimicrobial peptides (AMP) are versatile molecules synthesized by microorganisms using enzymatic pathways with no genetic code instruction. AMP have interesting properties in the food and pharmaceutical industries, like their antimicrobial ability against pathogens. Looking for biomolecules from microorganisms requires hard and time consuming chemical analysis of each microorganism extract. The microorganism isolation method proposed in this research allowed us to find antimicrobial peptides produced by bacteria, through interaction between a charged dye mixed with selective agar and metabolites produced by microorganisms. Twenty soil samples from different zones were isolated in selective media; thirty five strains were purified based on interaction between basic dye and charged molecules from bacteria. Streptomyces sp. y Bacillus sp. both genera were identified. Protein extracts were obtained from the isolated microorganisms cultivated in liquid media; peptides and amino acids were identified by thin layer chromatography and electrophoresis. Those extracts with high protein level were used to evaluate bioautography. Two extracts from 35 showed inhibitory activity against E. coli ATCC 8739 (8 mm halo). Method effectiveness for the isolation and the purifying of microorganisms able to produce charged molecules, of industrial interest is demonstrated. |
| first_indexed | 2024-12-14T08:02:59Z |
| format | Article |
| id | doaj.art-e812c59cd8204430ab3b4100c3c4aee0 |
| institution | Directory Open Access Journal |
| issn | 0121-4004 2145-2660 |
| language | English |
| last_indexed | 2024-12-14T08:02:59Z |
| publishDate | 2010-07-01 |
| publisher | Universidad de Antioquia |
| record_format | Article |
| series | Vitae |
| spelling | doaj.art-e812c59cd8204430ab3b4100c3c4aee02022-12-21T23:10:18ZengUniversidad de AntioquiaVitae0121-40042145-26602010-07-01172PEPTIDES WITH ANTIMICROBIAL ACTIVITY PRODUCED BY ISOLATED NATIVE MICROORGANISMSLigia L. CORRALES-GARCÍA0Gelmy L. CIRO G.1Facultad de Química Farmacéutica. Universidad de Antioquia.Programa de Ofidismo/Escorpionismo. Universidad de Antioquia.Like proteins, antimicrobial peptides (AMP) are versatile molecules synthesized by microorganisms using enzymatic pathways with no genetic code instruction. AMP have interesting properties in the food and pharmaceutical industries, like their antimicrobial ability against pathogens. Looking for biomolecules from microorganisms requires hard and time consuming chemical analysis of each microorganism extract. The microorganism isolation method proposed in this research allowed us to find antimicrobial peptides produced by bacteria, through interaction between a charged dye mixed with selective agar and metabolites produced by microorganisms. Twenty soil samples from different zones were isolated in selective media; thirty five strains were purified based on interaction between basic dye and charged molecules from bacteria. Streptomyces sp. y Bacillus sp. both genera were identified. Protein extracts were obtained from the isolated microorganisms cultivated in liquid media; peptides and amino acids were identified by thin layer chromatography and electrophoresis. Those extracts with high protein level were used to evaluate bioautography. Two extracts from 35 showed inhibitory activity against E. coli ATCC 8739 (8 mm halo). Method effectiveness for the isolation and the purifying of microorganisms able to produce charged molecules, of industrial interest is demonstrated.https://revistas.udea.edu.co/index.php/vitae/article/view/6343antimicrobial cationic peptidessoil microbiologythin layer chromatography |
| spellingShingle | Ligia L. CORRALES-GARCÍA Gelmy L. CIRO G. PEPTIDES WITH ANTIMICROBIAL ACTIVITY PRODUCED BY ISOLATED NATIVE MICROORGANISMS Vitae antimicrobial cationic peptides soil microbiology thin layer chromatography |
| title | PEPTIDES WITH ANTIMICROBIAL ACTIVITY PRODUCED BY ISOLATED NATIVE MICROORGANISMS |
| title_full | PEPTIDES WITH ANTIMICROBIAL ACTIVITY PRODUCED BY ISOLATED NATIVE MICROORGANISMS |
| title_fullStr | PEPTIDES WITH ANTIMICROBIAL ACTIVITY PRODUCED BY ISOLATED NATIVE MICROORGANISMS |
| title_full_unstemmed | PEPTIDES WITH ANTIMICROBIAL ACTIVITY PRODUCED BY ISOLATED NATIVE MICROORGANISMS |
| title_short | PEPTIDES WITH ANTIMICROBIAL ACTIVITY PRODUCED BY ISOLATED NATIVE MICROORGANISMS |
| title_sort | peptides with antimicrobial activity produced by isolated native microorganisms |
| topic | antimicrobial cationic peptides soil microbiology thin layer chromatography |
| url | https://revistas.udea.edu.co/index.php/vitae/article/view/6343 |
| work_keys_str_mv | AT ligialcorralesgarcia peptideswithantimicrobialactivityproducedbyisolatednativemicroorganisms AT gelmylcirog peptideswithantimicrobialactivityproducedbyisolatednativemicroorganisms |