Comparative Genomics of Prophages Sato and Sole Expands the Genetic Diversity Found in the Genus <i>Betatectivirus</i>
Tectiviruses infecting the <i>Bacillus cereus</i> group represent part of the bacterial “plasmid repertoire” as they behave as linear plasmids during their lysogenic cycle. Several novel tectiviruses have been recently found infecting diverse strains belonging the <i>B. cereus</...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/6/1335 |
| _version_ | 1797529528927518720 |
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| author | Annika Gillis Louise Hock Jacques Mahillon |
| author_facet | Annika Gillis Louise Hock Jacques Mahillon |
| author_sort | Annika Gillis |
| collection | DOAJ |
| description | Tectiviruses infecting the <i>Bacillus cereus</i> group represent part of the bacterial “plasmid repertoire” as they behave as linear plasmids during their lysogenic cycle. Several novel tectiviruses have been recently found infecting diverse strains belonging the <i>B. cereus</i> lineage. Here, we report and analyze the complete genome sequences of phages Sato and Sole. The linear dsDNA genome of Sato spans 14,852 bp with 32 coding DNA sequences (CDSs), whereas the one of Sole has 14,444 bp comprising 30 CDSs. Both phage genomes contain inverted terminal repeats and no tRNAs. Genomic comparisons and phylogenetic analyses placed these two phages within the genus <i>Betatectivirus</i> in the family <i>Tectiviridae</i>. Additional comparative genomic analyses indicated that the “gene regulation-genome replication” module of phages Sato and Sole is more diverse than previously observed among other fully sequenced betatectiviruses, displaying very low sequence similarities and containing some ORFans. Interestingly, the ssDNA binding protein encoded in this genomic module in phages Sato and Sole has very little amino acid similarity with those of reference betatectiviruses. Phylogenetic analyses showed that both Sato and Sole represent novel tectivirus species, thus we propose to include them as two novel species in the genus <i>Betatectivirus</i>. |
| first_indexed | 2024-03-10T10:15:07Z |
| format | Article |
| id | doaj.art-e8134c1609654b61b173705151da7bc0 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T10:15:07Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-e8134c1609654b61b173705151da7bc02023-11-22T00:53:46ZengMDPI AGMicroorganisms2076-26072021-06-0196133510.3390/microorganisms9061335Comparative Genomics of Prophages Sato and Sole Expands the Genetic Diversity Found in the Genus <i>Betatectivirus</i>Annika Gillis0Louise Hock1Jacques Mahillon2Laboratory of Food and Environmental Microbiology, Earth and Life Institute, UCLouvain, Croix du Sud 2, L7.05.12, B-1348 Louvain-la-Neuve, BelgiumLaboratory of Food and Environmental Microbiology, Earth and Life Institute, UCLouvain, Croix du Sud 2, L7.05.12, B-1348 Louvain-la-Neuve, BelgiumLaboratory of Food and Environmental Microbiology, Earth and Life Institute, UCLouvain, Croix du Sud 2, L7.05.12, B-1348 Louvain-la-Neuve, BelgiumTectiviruses infecting the <i>Bacillus cereus</i> group represent part of the bacterial “plasmid repertoire” as they behave as linear plasmids during their lysogenic cycle. Several novel tectiviruses have been recently found infecting diverse strains belonging the <i>B. cereus</i> lineage. Here, we report and analyze the complete genome sequences of phages Sato and Sole. The linear dsDNA genome of Sato spans 14,852 bp with 32 coding DNA sequences (CDSs), whereas the one of Sole has 14,444 bp comprising 30 CDSs. Both phage genomes contain inverted terminal repeats and no tRNAs. Genomic comparisons and phylogenetic analyses placed these two phages within the genus <i>Betatectivirus</i> in the family <i>Tectiviridae</i>. Additional comparative genomic analyses indicated that the “gene regulation-genome replication” module of phages Sato and Sole is more diverse than previously observed among other fully sequenced betatectiviruses, displaying very low sequence similarities and containing some ORFans. Interestingly, the ssDNA binding protein encoded in this genomic module in phages Sato and Sole has very little amino acid similarity with those of reference betatectiviruses. Phylogenetic analyses showed that both Sato and Sole represent novel tectivirus species, thus we propose to include them as two novel species in the genus <i>Betatectivirus</i>.https://www.mdpi.com/2076-2607/9/6/1335<i>Bacillus</i> phagesbetatectiviruseslysogenyplasmidial prophagestectivirustemperate phages |
| spellingShingle | Annika Gillis Louise Hock Jacques Mahillon Comparative Genomics of Prophages Sato and Sole Expands the Genetic Diversity Found in the Genus <i>Betatectivirus</i> Microorganisms <i>Bacillus</i> phages betatectiviruses lysogeny plasmidial prophages tectivirus temperate phages |
| title | Comparative Genomics of Prophages Sato and Sole Expands the Genetic Diversity Found in the Genus <i>Betatectivirus</i> |
| title_full | Comparative Genomics of Prophages Sato and Sole Expands the Genetic Diversity Found in the Genus <i>Betatectivirus</i> |
| title_fullStr | Comparative Genomics of Prophages Sato and Sole Expands the Genetic Diversity Found in the Genus <i>Betatectivirus</i> |
| title_full_unstemmed | Comparative Genomics of Prophages Sato and Sole Expands the Genetic Diversity Found in the Genus <i>Betatectivirus</i> |
| title_short | Comparative Genomics of Prophages Sato and Sole Expands the Genetic Diversity Found in the Genus <i>Betatectivirus</i> |
| title_sort | comparative genomics of prophages sato and sole expands the genetic diversity found in the genus i betatectivirus i |
| topic | <i>Bacillus</i> phages betatectiviruses lysogeny plasmidial prophages tectivirus temperate phages |
| url | https://www.mdpi.com/2076-2607/9/6/1335 |
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