Canine and Feline Testicular Preservation

The increased interest in breeding dogs and cats and their use as models for other canids and felids demand research to improve reproductive techniques. Among them, testicular cryopreservation stands out. Testicular cryopreservation enables the maintenance of reproductive capacity and allows the est...

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Main Author: Lúcia Daniel Machado da Silva
Format: Article
Language:English
Published: MDPI AG 2022-01-01
Series:Animals
Subjects:
Online Access:https://www.mdpi.com/2076-2615/12/1/124
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author Lúcia Daniel Machado da Silva
author_facet Lúcia Daniel Machado da Silva
author_sort Lúcia Daniel Machado da Silva
collection DOAJ
description The increased interest in breeding dogs and cats and their use as models for other canids and felids demand research to improve reproductive techniques. Among them, testicular cryopreservation stands out. Testicular cryopreservation enables the maintenance of reproductive capacity and allows the establishment of germplasm banks for several species of commercial value or at risk of extinction. Furthermore, it enables the transport of genetic material among different regions. It is noteworthy that this biotechnology represents the only possibility of preserving the fertility of prepubertal animals that have died, so it has great importance in the propagation of the genetic material of animals. The spermatogonia present in the testes can be cultivated in vitro and the sperm obtained can be used in artificial reproduction programs. Although advances have been achieved with the use of testicular fragments to obtain viable and functional germ cells, the establishment of protocols that can be used in clinical routine have not been concluded yet. The testicular cryopreservation process can be carried out through techniques such as slow freezing, fast freezing and vitrification. However, the protocols used for the canine and feline species are still in the experimental phase. Given the importance of the topic, the aim of this review is to draw a profile of the subject approaching the main works on testicular cryopreservation in dogs and cats.
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spelling doaj.art-e817f211ece1447f816a11c0452a2ff52023-11-23T11:06:05ZengMDPI AGAnimals2076-26152022-01-0112112410.3390/ani12010124Canine and Feline Testicular PreservationLúcia Daniel Machado da Silva0Laboratory of Carnivores Reproduction, Faculty of Veterinary Medicine, State University of Ceará, Fortaleza 60714-903, Ceará, BrazilThe increased interest in breeding dogs and cats and their use as models for other canids and felids demand research to improve reproductive techniques. Among them, testicular cryopreservation stands out. Testicular cryopreservation enables the maintenance of reproductive capacity and allows the establishment of germplasm banks for several species of commercial value or at risk of extinction. Furthermore, it enables the transport of genetic material among different regions. It is noteworthy that this biotechnology represents the only possibility of preserving the fertility of prepubertal animals that have died, so it has great importance in the propagation of the genetic material of animals. The spermatogonia present in the testes can be cultivated in vitro and the sperm obtained can be used in artificial reproduction programs. Although advances have been achieved with the use of testicular fragments to obtain viable and functional germ cells, the establishment of protocols that can be used in clinical routine have not been concluded yet. The testicular cryopreservation process can be carried out through techniques such as slow freezing, fast freezing and vitrification. However, the protocols used for the canine and feline species are still in the experimental phase. Given the importance of the topic, the aim of this review is to draw a profile of the subject approaching the main works on testicular cryopreservation in dogs and cats.https://www.mdpi.com/2076-2615/12/1/124dogcattestesconservationvitrificationfreezing
spellingShingle Lúcia Daniel Machado da Silva
Canine and Feline Testicular Preservation
Animals
dog
cat
testes
conservation
vitrification
freezing
title Canine and Feline Testicular Preservation
title_full Canine and Feline Testicular Preservation
title_fullStr Canine and Feline Testicular Preservation
title_full_unstemmed Canine and Feline Testicular Preservation
title_short Canine and Feline Testicular Preservation
title_sort canine and feline testicular preservation
topic dog
cat
testes
conservation
vitrification
freezing
url https://www.mdpi.com/2076-2615/12/1/124
work_keys_str_mv AT luciadanielmachadodasilva canineandfelinetesticularpreservation