Rapid separation and identification of 31 major saponins in Shizhu ginseng by ultra-high performance liquid chromatography–electron spray ionization–MS/MS
Background: Among the various ginseng strains, Shizhu ginseng is endemic to China, mainly distributed in Kuandian Manchu Autonomous County (Liaoning Province, China); however, not much is known about the compounds (especially saponins) in Shizhu ginseng. Methods: A rapid, sensitive, and reliable ult...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016-07-01
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| Series: | Journal of Ginseng Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1226845315000755 |
| _version_ | 1818534470456705024 |
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| author | Ting-Ting Sun Xin-Lei Liang He-Yun Zhu Xu-Ling Peng Xing-Jie Guo Long-Shan Zhao |
| author_facet | Ting-Ting Sun Xin-Lei Liang He-Yun Zhu Xu-Ling Peng Xing-Jie Guo Long-Shan Zhao |
| author_sort | Ting-Ting Sun |
| collection | DOAJ |
| description | Background: Among the various ginseng strains, Shizhu ginseng is endemic to China, mainly distributed in Kuandian Manchu Autonomous County (Liaoning Province, China); however, not much is known about the compounds (especially saponins) in Shizhu ginseng.
Methods: A rapid, sensitive, and reliable ultra-high performance liquid chromatography coupled with MS/MS (UHPLC–MS/MS) method was developed to separate and identify saponins in Shizhu ginseng.
Results: The separation was carried out on a Waters ACQUITY UPLC BEH C18 column (100 mm × 2.1 mm, 1.7 μm) with acetonitrile and 0.1% formic acid aqueous solution as the mobile phase under a gradient elution at 40°C. The detection was performed on a Micromass Quattro Micro API mass spectrometer equipped with electrospray ionization source in both positive and negative modes. Under the optimized conditions, a total of 31 saponins were identified or tentatively characterized by comparing retention time and MS data with related literatures and reference substances.
Conclusion: The developed UHPLC–MS/MS method was suitable for identifying and characterizing the chemical constituents in Shizhu ginseng, which provided a helpful chemical basis for further research on Shizhu ginseng. |
| first_indexed | 2024-12-11T18:12:03Z |
| format | Article |
| id | doaj.art-e8188ebdd5d347e4b503c87243cb8d9d |
| institution | Directory Open Access Journal |
| issn | 1226-8453 |
| language | English |
| last_indexed | 2024-12-11T18:12:03Z |
| publishDate | 2016-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Ginseng Research |
| spelling | doaj.art-e8188ebdd5d347e4b503c87243cb8d9d2022-12-22T00:55:33ZengElsevierJournal of Ginseng Research1226-84532016-07-0140322022810.1016/j.jgr.2015.07.008Rapid separation and identification of 31 major saponins in Shizhu ginseng by ultra-high performance liquid chromatography–electron spray ionization–MS/MSTing-Ting SunXin-Lei LiangHe-Yun ZhuXu-Ling PengXing-Jie GuoLong-Shan ZhaoBackground: Among the various ginseng strains, Shizhu ginseng is endemic to China, mainly distributed in Kuandian Manchu Autonomous County (Liaoning Province, China); however, not much is known about the compounds (especially saponins) in Shizhu ginseng. Methods: A rapid, sensitive, and reliable ultra-high performance liquid chromatography coupled with MS/MS (UHPLC–MS/MS) method was developed to separate and identify saponins in Shizhu ginseng. Results: The separation was carried out on a Waters ACQUITY UPLC BEH C18 column (100 mm × 2.1 mm, 1.7 μm) with acetonitrile and 0.1% formic acid aqueous solution as the mobile phase under a gradient elution at 40°C. The detection was performed on a Micromass Quattro Micro API mass spectrometer equipped with electrospray ionization source in both positive and negative modes. Under the optimized conditions, a total of 31 saponins were identified or tentatively characterized by comparing retention time and MS data with related literatures and reference substances. Conclusion: The developed UHPLC–MS/MS method was suitable for identifying and characterizing the chemical constituents in Shizhu ginseng, which provided a helpful chemical basis for further research on Shizhu ginseng.http://www.sciencedirect.com/science/article/pii/S1226845315000755identificationsaponinsseparationShizhu ginsengultra-high performance liquid chromatography coupled with MS/MS |
| spellingShingle | Ting-Ting Sun Xin-Lei Liang He-Yun Zhu Xu-Ling Peng Xing-Jie Guo Long-Shan Zhao Rapid separation and identification of 31 major saponins in Shizhu ginseng by ultra-high performance liquid chromatography–electron spray ionization–MS/MS Journal of Ginseng Research identification saponins separation Shizhu ginseng ultra-high performance liquid chromatography coupled with MS/MS |
| title | Rapid separation and identification of 31 major saponins in Shizhu ginseng by ultra-high performance liquid chromatography–electron spray ionization–MS/MS |
| title_full | Rapid separation and identification of 31 major saponins in Shizhu ginseng by ultra-high performance liquid chromatography–electron spray ionization–MS/MS |
| title_fullStr | Rapid separation and identification of 31 major saponins in Shizhu ginseng by ultra-high performance liquid chromatography–electron spray ionization–MS/MS |
| title_full_unstemmed | Rapid separation and identification of 31 major saponins in Shizhu ginseng by ultra-high performance liquid chromatography–electron spray ionization–MS/MS |
| title_short | Rapid separation and identification of 31 major saponins in Shizhu ginseng by ultra-high performance liquid chromatography–electron spray ionization–MS/MS |
| title_sort | rapid separation and identification of 31 major saponins in shizhu ginseng by ultra high performance liquid chromatography electron spray ionization ms ms |
| topic | identification saponins separation Shizhu ginseng ultra-high performance liquid chromatography coupled with MS/MS |
| url | http://www.sciencedirect.com/science/article/pii/S1226845315000755 |
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