MicroRNA-127 and MicroRNA-132 Expression in Patients with Methamphetamine Abuse in East Azerbaijan, Iran: A Case-Control Study
Background: Addiction is a personal and social problem worldwide, and has physical and psychological effects on consumers’ health. Recently, miRNAs have been described as noninvasive biomarkers. Currently, methamphetamine abuse (MA) is mainly diagnosed by chromatography. This study aimed to investig...
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| Format: | Article |
| Language: | English |
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Kerman University of Medical Sciences
2022-07-01
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| Series: | Addiction and Health |
| Subjects: | |
| Online Access: | https://ahj.kmu.ac.ir/article_92054_bdf86a53c932a986df4fbbdc6e430cca.pdf |
| _version_ | 1827825344678199296 |
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| author | Saman Rezai Moradali Hossein Soltanzadeh Hassan Montazam Zahra Asadi Shima Fathi |
| author_facet | Saman Rezai Moradali Hossein Soltanzadeh Hassan Montazam Zahra Asadi Shima Fathi |
| author_sort | Saman Rezai Moradali |
| collection | DOAJ |
| description | Background: Addiction is a personal and social problem worldwide, and has physical and psychological effects on consumers’ health. Recently, miRNAs have been described as noninvasive biomarkers. Currently, methamphetamine abuse (MA) is mainly diagnosed by chromatography. This study aimed to investigate the expression and diagnostic value of miR-127 and miR-132 in blood samples of patients with MA and non-user healthy controls.
Methods: A total of 60 patients with MA (case group) and 60 non-user healthy individuals (control group) were selected from Tabriz, East Azerbaijan, Iran. Peripheral blood was obtained and total RNA was extracted. Then, cDNA synthesis was performed and miR-127 and miR-132 expression was evaluated using real time polymerase chain reaction (PCR) method.
Findings: The results of this study demonstrated that miR-127 was significantly lower (0.042-fold change) in patients with MA than in the control group (P<0.05). However, miR-132 was significantly higher (7.1-fold change) in patients with MA than in the control group (P<0.05).
Conclusion: In general, expression of miR-127 and miR-132 may alter in patients with MA. Further studies are needed to identify underlying molecular mechanisms in patients with MA. |
| first_indexed | 2024-03-12T02:47:44Z |
| format | Article |
| id | doaj.art-e81c477ae6664ced8156f1ac6a288d61 |
| institution | Directory Open Access Journal |
| issn | 2008-4633 2008-8469 |
| language | English |
| last_indexed | 2024-03-12T02:47:44Z |
| publishDate | 2022-07-01 |
| publisher | Kerman University of Medical Sciences |
| record_format | Article |
| series | Addiction and Health |
| spelling | doaj.art-e81c477ae6664ced8156f1ac6a288d612023-09-04T06:42:11ZengKerman University of Medical SciencesAddiction and Health2008-46332008-84692022-07-011432141710.34172/ahj.2022.129892054MicroRNA-127 and MicroRNA-132 Expression in Patients with Methamphetamine Abuse in East Azerbaijan, Iran: A Case-Control StudySaman Rezai Moradali0Hossein Soltanzadeh1Hassan Montazam2Zahra Asadi3Shima Fathi4Department of Genetics, Bonab Branch, Islamic Azad University, Bonab, Iran1. Department of Genetics, Bonab Branch, Islamic Azad University, Bonab, Iran 2. Medicinal Plants Research Center, Maragheh University of Medical Sciences, Maragheh, IranDepartment of Genetics, Bonab Branch, Islamic Azad University, Bonab, IranDepartment of Genetics, Bonab Branch, Islamic Azad University, Bonab, IranDepartment of Genetics, Bonab Branch, Islamic Azad University, Bonab, IranBackground: Addiction is a personal and social problem worldwide, and has physical and psychological effects on consumers’ health. Recently, miRNAs have been described as noninvasive biomarkers. Currently, methamphetamine abuse (MA) is mainly diagnosed by chromatography. This study aimed to investigate the expression and diagnostic value of miR-127 and miR-132 in blood samples of patients with MA and non-user healthy controls. Methods: A total of 60 patients with MA (case group) and 60 non-user healthy individuals (control group) were selected from Tabriz, East Azerbaijan, Iran. Peripheral blood was obtained and total RNA was extracted. Then, cDNA synthesis was performed and miR-127 and miR-132 expression was evaluated using real time polymerase chain reaction (PCR) method. Findings: The results of this study demonstrated that miR-127 was significantly lower (0.042-fold change) in patients with MA than in the control group (P<0.05). However, miR-132 was significantly higher (7.1-fold change) in patients with MA than in the control group (P<0.05). Conclusion: In general, expression of miR-127 and miR-132 may alter in patients with MA. Further studies are needed to identify underlying molecular mechanisms in patients with MA.https://ahj.kmu.ac.ir/article_92054_bdf86a53c932a986df4fbbdc6e430cca.pdfaddictionmethamphetaminemicrorna-127microrna-132biomarker |
| spellingShingle | Saman Rezai Moradali Hossein Soltanzadeh Hassan Montazam Zahra Asadi Shima Fathi MicroRNA-127 and MicroRNA-132 Expression in Patients with Methamphetamine Abuse in East Azerbaijan, Iran: A Case-Control Study Addiction and Health addiction methamphetamine microrna-127 microrna-132 biomarker |
| title | MicroRNA-127 and MicroRNA-132 Expression in Patients with Methamphetamine Abuse in East Azerbaijan, Iran: A Case-Control Study |
| title_full | MicroRNA-127 and MicroRNA-132 Expression in Patients with Methamphetamine Abuse in East Azerbaijan, Iran: A Case-Control Study |
| title_fullStr | MicroRNA-127 and MicroRNA-132 Expression in Patients with Methamphetamine Abuse in East Azerbaijan, Iran: A Case-Control Study |
| title_full_unstemmed | MicroRNA-127 and MicroRNA-132 Expression in Patients with Methamphetamine Abuse in East Azerbaijan, Iran: A Case-Control Study |
| title_short | MicroRNA-127 and MicroRNA-132 Expression in Patients with Methamphetamine Abuse in East Azerbaijan, Iran: A Case-Control Study |
| title_sort | microrna 127 and microrna 132 expression in patients with methamphetamine abuse in east azerbaijan iran a case control study |
| topic | addiction methamphetamine microrna-127 microrna-132 biomarker |
| url | https://ahj.kmu.ac.ir/article_92054_bdf86a53c932a986df4fbbdc6e430cca.pdf |
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