A retrospective analysis of serological & molecular testing data on dengue fever in Kolkata & adjacent districts during 2016-2019
Background & objectives: Regional Virus Research and Diagnostic Laboratory established at ICMR-National Institute of Cholera and Enteric Diseases (NICED) regularly receives samples for dengue screening and serotyping from patients of acute febrile illness (AFI) from Kolkata and adjacent district...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2022-01-01
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| Series: | Indian Journal of Medical Research |
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| Online Access: | http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2022;volume=156;issue=4;spage=608;epage=614;aulast=Majumdar |
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| author | Agniva Majumdar Rajarshi Gupta Ananya Chatterjee Hasina Banu Madhumonti Biswas Rudrak Gupta Soumen Mukherjee Provash Sadhukhan Shanta Dutta |
| author_facet | Agniva Majumdar Rajarshi Gupta Ananya Chatterjee Hasina Banu Madhumonti Biswas Rudrak Gupta Soumen Mukherjee Provash Sadhukhan Shanta Dutta |
| author_sort | Agniva Majumdar |
| collection | DOAJ |
| description | Background & objectives: Regional Virus Research and Diagnostic Laboratory established at ICMR-National Institute of Cholera and Enteric Diseases (NICED) regularly receives samples for dengue screening and serotyping from patients of acute febrile illness (AFI) from Kolkata and adjacent districts. In this study, data over a three year period (August 2016-July 2019) was retrospectively analyzed to provide insight into the epidemiological trends of dengue fever in this region.
Methods: Serological screening of dengue was performed by detection of NS1 antigen and/or immunoglobulin M (IgM) antibody. Dengue serotyping was done by conventional or real-time reverse transcriptase–PCR. The data were analyzed to describe the distribution of dengue with respect to age of patient, duration of fever on the day of blood collection and month of the year. Zip codes were used for spatial plotting.
Results: Out of the 24,474 samples received from Kolkata and its adjacent districts (Hooghly, Howrah, North and South 24 Parganas), 38.3 per cent (95% confidence interval: 37.7-38.9%) samples were screened positive for dengue. The correlation between age and dengue positivity was found to be weak. A combination of dengue NS1 antigen and dengue IgM antibody detection may be a better option for detecting dengue positivity compared to a single test. Most AFI cases were tested from August to November during the study period, with maximum dengue positivity noted during September (45.9%). The predominant serotype of 2016, dengue virus serotype 1 (DENV-1), was almost entirely replaced by DENV-2 in 2017 and 2018.
Interpretation & conclusions: Dengue continues to be an important cause of AFI in the region and round-the-year preventive measures are required for its control. Serotype switching is alarming and should be monitored routinely. |
| first_indexed | 2024-03-13T10:37:33Z |
| format | Article |
| id | doaj.art-e81dfb4c0967436d949a274b1ed402c0 |
| institution | Directory Open Access Journal |
| issn | 0971-5916 |
| language | English |
| last_indexed | 2024-03-13T10:37:33Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Indian Journal of Medical Research |
| spelling | doaj.art-e81dfb4c0967436d949a274b1ed402c02023-05-18T05:47:41ZengWolters Kluwer Medknow PublicationsIndian Journal of Medical Research0971-59162022-01-01156460861410.4103/ijmr.IJMR_2612_19A retrospective analysis of serological & molecular testing data on dengue fever in Kolkata & adjacent districts during 2016-2019Agniva MajumdarRajarshi GuptaAnanya ChatterjeeHasina BanuMadhumonti BiswasRudrak GuptaSoumen MukherjeeProvash SadhukhanShanta DuttaBackground & objectives: Regional Virus Research and Diagnostic Laboratory established at ICMR-National Institute of Cholera and Enteric Diseases (NICED) regularly receives samples for dengue screening and serotyping from patients of acute febrile illness (AFI) from Kolkata and adjacent districts. In this study, data over a three year period (August 2016-July 2019) was retrospectively analyzed to provide insight into the epidemiological trends of dengue fever in this region. Methods: Serological screening of dengue was performed by detection of NS1 antigen and/or immunoglobulin M (IgM) antibody. Dengue serotyping was done by conventional or real-time reverse transcriptase–PCR. The data were analyzed to describe the distribution of dengue with respect to age of patient, duration of fever on the day of blood collection and month of the year. Zip codes were used for spatial plotting. Results: Out of the 24,474 samples received from Kolkata and its adjacent districts (Hooghly, Howrah, North and South 24 Parganas), 38.3 per cent (95% confidence interval: 37.7-38.9%) samples were screened positive for dengue. The correlation between age and dengue positivity was found to be weak. A combination of dengue NS1 antigen and dengue IgM antibody detection may be a better option for detecting dengue positivity compared to a single test. Most AFI cases were tested from August to November during the study period, with maximum dengue positivity noted during September (45.9%). The predominant serotype of 2016, dengue virus serotype 1 (DENV-1), was almost entirely replaced by DENV-2 in 2017 and 2018. Interpretation & conclusions: Dengue continues to be an important cause of AFI in the region and round-the-year preventive measures are required for its control. Serotype switching is alarming and should be monitored routinely.http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2022;volume=156;issue=4;spage=608;epage=614;aulast=Majumdardengue fever - dengue serotyping - kolkata - mapping |
| spellingShingle | Agniva Majumdar Rajarshi Gupta Ananya Chatterjee Hasina Banu Madhumonti Biswas Rudrak Gupta Soumen Mukherjee Provash Sadhukhan Shanta Dutta A retrospective analysis of serological & molecular testing data on dengue fever in Kolkata & adjacent districts during 2016-2019 Indian Journal of Medical Research dengue fever - dengue serotyping - kolkata - mapping |
| title | A retrospective analysis of serological & molecular testing data on dengue fever in Kolkata & adjacent districts during 2016-2019 |
| title_full | A retrospective analysis of serological & molecular testing data on dengue fever in Kolkata & adjacent districts during 2016-2019 |
| title_fullStr | A retrospective analysis of serological & molecular testing data on dengue fever in Kolkata & adjacent districts during 2016-2019 |
| title_full_unstemmed | A retrospective analysis of serological & molecular testing data on dengue fever in Kolkata & adjacent districts during 2016-2019 |
| title_short | A retrospective analysis of serological & molecular testing data on dengue fever in Kolkata & adjacent districts during 2016-2019 |
| title_sort | retrospective analysis of serological molecular testing data on dengue fever in kolkata adjacent districts during 2016 2019 |
| topic | dengue fever - dengue serotyping - kolkata - mapping |
| url | http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2022;volume=156;issue=4;spage=608;epage=614;aulast=Majumdar |
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