Effect of fermentation on antibacterial activity of milk from Vechur and Kasargod Dwarf cattle
The search for alternative therapeutics is on the rise on account of widespread bacterial resistance to numerous conventional antibiotics. Bioactive peptides produced by lactic acid bacteria found in natural sources such as fermented dairy products have attracted attention as a possible source of bi...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Director of Academics and Research, Kerala Veterinary and Animal Sciences University
2023-12-01
|
| Series: | Journal of Veterinary and Animal Sciences |
| Subjects: | |
| Online Access: | https://jvas.in/public_html/upload/article_file/article_file_s6kkze.pdf?t=s6kkze |
| _version_ | 1827382993492115456 |
|---|---|
| author | J. S. Pooja C. R. Rachana S. Panicker Aiswarya K. J. Sana |
| author_facet | J. S. Pooja C. R. Rachana S. Panicker Aiswarya K. J. Sana |
| author_sort | J. S. Pooja |
| collection | DOAJ |
| description | The search for alternative therapeutics is on the rise on account of widespread bacterial resistance to numerous conventional antibiotics. Bioactive peptides produced by lactic acid bacteria found in natural sources such as fermented dairy products have attracted attention as a possible source of biotherapeutic peptides. Bioactive peptides released in fermented milk, on the other hand, is dependent on the proteolytic activities of the cultures used. As a result, this study was carried out to evaluate the effect of fermentation on antibacterial activity of milk from Vechur and Kasargod Dwarf cattle against pathogenic bacteria during 21-days storage period at 5°C. High-speed centrifugation was used to create water-soluble crude peptide extracts. The water-soluble peptides from Kasargod Dwarf and Vechur cattle by fermentation showed maximum inhibition against Escherichia coli NCIM2685 and Salmonella enterica ATCC 6017. The zone of inhibition measured ranges from 5mm to 11mm throughout the storage period. However, both did not show zone of inhibition against Bacillus cereus ATCC 10876 and Staphylococcus aureus ATCC 25923 during 21 days of storage at pH 7.4. |
| first_indexed | 2024-03-08T14:31:30Z |
| format | Article |
| id | doaj.art-e81f7e05bd364e258e60ad326a6cedb7 |
| institution | Directory Open Access Journal |
| issn | 0971-0701 2582-0605 |
| language | English |
| last_indexed | 2024-03-08T14:31:30Z |
| publishDate | 2023-12-01 |
| publisher | Director of Academics and Research, Kerala Veterinary and Animal Sciences University |
| record_format | Article |
| series | Journal of Veterinary and Animal Sciences |
| spelling | doaj.art-e81f7e05bd364e258e60ad326a6cedb72024-01-12T10:56:03ZengDirector of Academics and Research, Kerala Veterinary and Animal Sciences UniversityJournal of Veterinary and Animal Sciences0971-07012582-06052023-12-0154496697210.51966/jvas.2023.54.4.966-972Effect of fermentation on antibacterial activity of milk from Vechur and Kasargod Dwarf cattleJ. S. Pooja0https://orcid.org/0009-0007-6698-6991C. R. Rachana1S. Panicker Aiswarya2K. J. Sana3M.Sc. Scholar,, Department of Veterinary Biochemistry, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651, Kerala Veterinary and Animal Sciences University, Kerala, IndiaAssistant professor, Dept. of Dairy Chemistry, College of Dairy Science and Technology, Thiruvananthapuram -695040.Assistant professor, Dept. of Dairy Microbiology, College of Dairy Science and Technology, Thiruvananthapuram -695040.M-Tech Scholar, Dept. Of Dairy Chemistry, Verghese Kurien Institute of Dairy and Food Technology, Mannuthy, Thrissur- 680 651.The search for alternative therapeutics is on the rise on account of widespread bacterial resistance to numerous conventional antibiotics. Bioactive peptides produced by lactic acid bacteria found in natural sources such as fermented dairy products have attracted attention as a possible source of biotherapeutic peptides. Bioactive peptides released in fermented milk, on the other hand, is dependent on the proteolytic activities of the cultures used. As a result, this study was carried out to evaluate the effect of fermentation on antibacterial activity of milk from Vechur and Kasargod Dwarf cattle against pathogenic bacteria during 21-days storage period at 5°C. High-speed centrifugation was used to create water-soluble crude peptide extracts. The water-soluble peptides from Kasargod Dwarf and Vechur cattle by fermentation showed maximum inhibition against Escherichia coli NCIM2685 and Salmonella enterica ATCC 6017. The zone of inhibition measured ranges from 5mm to 11mm throughout the storage period. However, both did not show zone of inhibition against Bacillus cereus ATCC 10876 and Staphylococcus aureus ATCC 25923 during 21 days of storage at pH 7.4.https://jvas.in/public_html/upload/article_file/article_file_s6kkze.pdf?t=s6kkzevechur cattlekasargod dwarfantibacterial activitybioactive peptides |
| spellingShingle | J. S. Pooja C. R. Rachana S. Panicker Aiswarya K. J. Sana Effect of fermentation on antibacterial activity of milk from Vechur and Kasargod Dwarf cattle Journal of Veterinary and Animal Sciences vechur cattle kasargod dwarf antibacterial activity bioactive peptides |
| title | Effect of fermentation on antibacterial activity of milk from Vechur and Kasargod Dwarf cattle |
| title_full | Effect of fermentation on antibacterial activity of milk from Vechur and Kasargod Dwarf cattle |
| title_fullStr | Effect of fermentation on antibacterial activity of milk from Vechur and Kasargod Dwarf cattle |
| title_full_unstemmed | Effect of fermentation on antibacterial activity of milk from Vechur and Kasargod Dwarf cattle |
| title_short | Effect of fermentation on antibacterial activity of milk from Vechur and Kasargod Dwarf cattle |
| title_sort | effect of fermentation on antibacterial activity of milk from vechur and kasargod dwarf cattle |
| topic | vechur cattle kasargod dwarf antibacterial activity bioactive peptides |
| url | https://jvas.in/public_html/upload/article_file/article_file_s6kkze.pdf?t=s6kkze |
| work_keys_str_mv | AT jspooja effectoffermentationonantibacterialactivityofmilkfromvechurandkasargoddwarfcattle AT crrachana effectoffermentationonantibacterialactivityofmilkfromvechurandkasargoddwarfcattle AT spanickeraiswarya effectoffermentationonantibacterialactivityofmilkfromvechurandkasargoddwarfcattle AT kjsana effectoffermentationonantibacterialactivityofmilkfromvechurandkasargoddwarfcattle |