Gastric Carcinomas with Stromal B7-H3 Expression Have Lower Intratumoural CD8+ T Cell Density
CD8+ T cells are the main effector cells of anti-cancer immune response that can be regulated by various costimulatory and coinhibitory molecules, including members of the B7 family. B7 homolog 3 (B7-H3) appears as a promising marker for immunotherapy; however, its significance in gastric cancer (GC...
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MDPI AG
2021-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/4/2129 |
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| author | Dita Ulase Hans-Michael Behrens Sandra Krüger Sebastian Zeissig Christoph Röcken |
| author_facet | Dita Ulase Hans-Michael Behrens Sandra Krüger Sebastian Zeissig Christoph Röcken |
| author_sort | Dita Ulase |
| collection | DOAJ |
| description | CD8+ T cells are the main effector cells of anti-cancer immune response that can be regulated by various costimulatory and coinhibitory molecules, including members of the B7 family. B7 homolog 3 (B7-H3) appears as a promising marker for immunotherapy; however, its significance in gastric cancer (GC) is unclear yet. We evaluated the spatial distribution of CD8+ T cells in relation to the expression of B7-H3 by double immunohistochemical staining. The level of B7-H3 intensity was scored manually (0–3) and dichotomized into B7-H3-low and B7-H3-high groups. The distribution and density of CD8+ T cells was analysed using whole slide digital imaging. B7-H3 was expressed mainly in the stromal compartment of GC (<i>n</i> = 73, 76% of all cases). Tumours with high expression of B7-H3 showed larger spatial differences of CD8+ T cells (86.4/mm<sup>2</sup> in tumour centre vs. 414.9/mm<sup>2</sup> in invasive front) when compared to B7-H3-low group (157.7/mm<sup>2</sup> vs. 218.7/mm<sup>2</sup>, respectively) (<i>p</i> < 0.001). This study provides insight into the expression pattern of B7-H3 in GC of Western origin. In GCs with higher level of B7-H3 expression, CD8+ T cells were spatially suppressed in the tumour centre suggesting that B7-H3 might be involved in tumour escape mechanisms from the immune response. |
| first_indexed | 2024-03-09T00:41:07Z |
| format | Article |
| id | doaj.art-e8218d603926476eb6ba0f8797e8d208 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T00:41:07Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-e8218d603926476eb6ba0f8797e8d2082023-12-11T17:51:31ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-02-01224212910.3390/ijms22042129Gastric Carcinomas with Stromal B7-H3 Expression Have Lower Intratumoural CD8+ T Cell DensityDita Ulase0Hans-Michael Behrens1Sandra Krüger2Sebastian Zeissig3Christoph Röcken4Department of Pathology, Christian-Albrechts-University, 24105 Kiel, GermanyDepartment of Pathology, Christian-Albrechts-University, 24105 Kiel, GermanyDepartment of Pathology, Christian-Albrechts-University, 24105 Kiel, GermanyDepartment of Medicine I, University Medical Center Dresden, Technische Universität (TU) Dresden, 01307 Dresden, GermanyDepartment of Pathology, Christian-Albrechts-University, 24105 Kiel, GermanyCD8+ T cells are the main effector cells of anti-cancer immune response that can be regulated by various costimulatory and coinhibitory molecules, including members of the B7 family. B7 homolog 3 (B7-H3) appears as a promising marker for immunotherapy; however, its significance in gastric cancer (GC) is unclear yet. We evaluated the spatial distribution of CD8+ T cells in relation to the expression of B7-H3 by double immunohistochemical staining. The level of B7-H3 intensity was scored manually (0–3) and dichotomized into B7-H3-low and B7-H3-high groups. The distribution and density of CD8+ T cells was analysed using whole slide digital imaging. B7-H3 was expressed mainly in the stromal compartment of GC (<i>n</i> = 73, 76% of all cases). Tumours with high expression of B7-H3 showed larger spatial differences of CD8+ T cells (86.4/mm<sup>2</sup> in tumour centre vs. 414.9/mm<sup>2</sup> in invasive front) when compared to B7-H3-low group (157.7/mm<sup>2</sup> vs. 218.7/mm<sup>2</sup>, respectively) (<i>p</i> < 0.001). This study provides insight into the expression pattern of B7-H3 in GC of Western origin. In GCs with higher level of B7-H3 expression, CD8+ T cells were spatially suppressed in the tumour centre suggesting that B7-H3 might be involved in tumour escape mechanisms from the immune response.https://www.mdpi.com/1422-0067/22/4/2129B7-H3CD8-Positive T-Lymphocytesgastric cancerimmune checkpointimmune evasionimmunohistochemistry |
| spellingShingle | Dita Ulase Hans-Michael Behrens Sandra Krüger Sebastian Zeissig Christoph Röcken Gastric Carcinomas with Stromal B7-H3 Expression Have Lower Intratumoural CD8+ T Cell Density International Journal of Molecular Sciences B7-H3 CD8-Positive T-Lymphocytes gastric cancer immune checkpoint immune evasion immunohistochemistry |
| title | Gastric Carcinomas with Stromal B7-H3 Expression Have Lower Intratumoural CD8+ T Cell Density |
| title_full | Gastric Carcinomas with Stromal B7-H3 Expression Have Lower Intratumoural CD8+ T Cell Density |
| title_fullStr | Gastric Carcinomas with Stromal B7-H3 Expression Have Lower Intratumoural CD8+ T Cell Density |
| title_full_unstemmed | Gastric Carcinomas with Stromal B7-H3 Expression Have Lower Intratumoural CD8+ T Cell Density |
| title_short | Gastric Carcinomas with Stromal B7-H3 Expression Have Lower Intratumoural CD8+ T Cell Density |
| title_sort | gastric carcinomas with stromal b7 h3 expression have lower intratumoural cd8 t cell density |
| topic | B7-H3 CD8-Positive T-Lymphocytes gastric cancer immune checkpoint immune evasion immunohistochemistry |
| url | https://www.mdpi.com/1422-0067/22/4/2129 |
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