Gene-Expression Patterns of Tumor and Peritumor Tissues of Smoking and Non-Smoking HPV-Negative Patients with Head and Neck Squamous Cell Carcinoma
We studied the gene-expression patterns in specimens of tumor and peritumor tissue biopsies of 26 patients with head and neck carcinomas depending on smoking status. Histological and immunohistochemical examinations verified that all tumors belonged to the “classical” subgroup of head and neck carci...
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MDPI AG
2024-03-01
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| Online Access: | https://www.mdpi.com/2227-9059/12/3/696 |
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| author | Anna Soboleva Irina Arutyunyan Enar Jumaniyazova Polina Vishnyakova Daria Zarubina Eldar Nimatov Andrey Elchaninov Timur Fatkhudinov |
| author_facet | Anna Soboleva Irina Arutyunyan Enar Jumaniyazova Polina Vishnyakova Daria Zarubina Eldar Nimatov Andrey Elchaninov Timur Fatkhudinov |
| author_sort | Anna Soboleva |
| collection | DOAJ |
| description | We studied the gene-expression patterns in specimens of tumor and peritumor tissue biopsies of 26 patients with head and neck carcinomas depending on smoking status. Histological and immunohistochemical examinations verified that all tumors belonged to the “classical” subgroup of head and neck carcinomas, and the HPV-negative tumor status was confirmed. The expression of 28 tumor-associated genes determined by RT-PCR was independent of patients’ sex or age, TNM status, degree of differentiation, or tissue localization. Moreover, in peritumor tissue, none of the 28 genes were differentially expressed between the groups of smoking and nonsmoking patients. During oncotransformation in both studied groups, there were similar processes typical for HNSCC progression: the expression levels of paired keratins 4 and 13 were reduced, while the expression levels of keratin 17 and <i>CD44</i> were significantly increased. However, further investigation revealed some distinctive features: the expression of the genes <i>EGFR</i> and <i>TP63</i> increased significantly only in the nonsmoking group, and the expression of <i>IL6</i>, <i>CDKN2A</i>, <i>EGF</i>, and <i>PITX1</i> genes changed only in the smoking group. In addition, correlation analysis identified several clusters within which genes displayed correlations in their expression levels. The largest group included 10 genes: <i>TIMP1</i>, <i>TIMP2</i>, <i>WEE1</i>, <i>YAP</i>, <i>HIF1A</i>, <i>PI3KCA</i>, <i>UTP14A</i>, <i>APIP</i>, <i>PTEN</i>, and <i>SLC26A6</i>. The genetic signatures associated with smoking habits that we have found may serve as a prerequisite for the development of diagnostic panels/tests predicting responses to different therapeutic strategies for HNSCC. |
| first_indexed | 2024-04-24T18:31:46Z |
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| language | English |
| last_indexed | 2024-04-24T18:31:46Z |
| publishDate | 2024-03-01 |
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| spelling | doaj.art-e82340e8ca5545a69226646c38dbe0cb2024-03-27T13:27:24ZengMDPI AGBiomedicines2227-90592024-03-0112369610.3390/biomedicines12030696Gene-Expression Patterns of Tumor and Peritumor Tissues of Smoking and Non-Smoking HPV-Negative Patients with Head and Neck Squamous Cell CarcinomaAnna Soboleva0Irina Arutyunyan1Enar Jumaniyazova2Polina Vishnyakova3Daria Zarubina4Eldar Nimatov5Andrey Elchaninov6Timur Fatkhudinov7Research Institute of Molecular and Cellular Medicine, Patrice Lumumba Peoples’ Friendship University of Russia (RUDN), 117198 Moscow, RussiaResearch Institute of Molecular and Cellular Medicine, Patrice Lumumba Peoples’ Friendship University of Russia (RUDN), 117198 Moscow, RussiaResearch Institute of Molecular and Cellular Medicine, Patrice Lumumba Peoples’ Friendship University of Russia (RUDN), 117198 Moscow, RussiaResearch Institute of Molecular and Cellular Medicine, Patrice Lumumba Peoples’ Friendship University of Russia (RUDN), 117198 Moscow, RussiaP. Hertsen Moscow Oncology Research Institute, National Medical Research Radiological Centre of the Ministry of Health of the Russian Federation, 125284 Moscow, RussiaP. Hertsen Moscow Oncology Research Institute, National Medical Research Radiological Centre of the Ministry of Health of the Russian Federation, 125284 Moscow, RussiaResearch Institute of Molecular and Cellular Medicine, Patrice Lumumba Peoples’ Friendship University of Russia (RUDN), 117198 Moscow, RussiaResearch Institute of Molecular and Cellular Medicine, Patrice Lumumba Peoples’ Friendship University of Russia (RUDN), 117198 Moscow, RussiaWe studied the gene-expression patterns in specimens of tumor and peritumor tissue biopsies of 26 patients with head and neck carcinomas depending on smoking status. Histological and immunohistochemical examinations verified that all tumors belonged to the “classical” subgroup of head and neck carcinomas, and the HPV-negative tumor status was confirmed. The expression of 28 tumor-associated genes determined by RT-PCR was independent of patients’ sex or age, TNM status, degree of differentiation, or tissue localization. Moreover, in peritumor tissue, none of the 28 genes were differentially expressed between the groups of smoking and nonsmoking patients. During oncotransformation in both studied groups, there were similar processes typical for HNSCC progression: the expression levels of paired keratins 4 and 13 were reduced, while the expression levels of keratin 17 and <i>CD44</i> were significantly increased. However, further investigation revealed some distinctive features: the expression of the genes <i>EGFR</i> and <i>TP63</i> increased significantly only in the nonsmoking group, and the expression of <i>IL6</i>, <i>CDKN2A</i>, <i>EGF</i>, and <i>PITX1</i> genes changed only in the smoking group. In addition, correlation analysis identified several clusters within which genes displayed correlations in their expression levels. The largest group included 10 genes: <i>TIMP1</i>, <i>TIMP2</i>, <i>WEE1</i>, <i>YAP</i>, <i>HIF1A</i>, <i>PI3KCA</i>, <i>UTP14A</i>, <i>APIP</i>, <i>PTEN</i>, and <i>SLC26A6</i>. The genetic signatures associated with smoking habits that we have found may serve as a prerequisite for the development of diagnostic panels/tests predicting responses to different therapeutic strategies for HNSCC.https://www.mdpi.com/2227-9059/12/3/696head and neck squamous cell carcinomatobacco smokingtumorperitumor tissuegene expression |
| spellingShingle | Anna Soboleva Irina Arutyunyan Enar Jumaniyazova Polina Vishnyakova Daria Zarubina Eldar Nimatov Andrey Elchaninov Timur Fatkhudinov Gene-Expression Patterns of Tumor and Peritumor Tissues of Smoking and Non-Smoking HPV-Negative Patients with Head and Neck Squamous Cell Carcinoma Biomedicines head and neck squamous cell carcinoma tobacco smoking tumor peritumor tissue gene expression |
| title | Gene-Expression Patterns of Tumor and Peritumor Tissues of Smoking and Non-Smoking HPV-Negative Patients with Head and Neck Squamous Cell Carcinoma |
| title_full | Gene-Expression Patterns of Tumor and Peritumor Tissues of Smoking and Non-Smoking HPV-Negative Patients with Head and Neck Squamous Cell Carcinoma |
| title_fullStr | Gene-Expression Patterns of Tumor and Peritumor Tissues of Smoking and Non-Smoking HPV-Negative Patients with Head and Neck Squamous Cell Carcinoma |
| title_full_unstemmed | Gene-Expression Patterns of Tumor and Peritumor Tissues of Smoking and Non-Smoking HPV-Negative Patients with Head and Neck Squamous Cell Carcinoma |
| title_short | Gene-Expression Patterns of Tumor and Peritumor Tissues of Smoking and Non-Smoking HPV-Negative Patients with Head and Neck Squamous Cell Carcinoma |
| title_sort | gene expression patterns of tumor and peritumor tissues of smoking and non smoking hpv negative patients with head and neck squamous cell carcinoma |
| topic | head and neck squamous cell carcinoma tobacco smoking tumor peritumor tissue gene expression |
| url | https://www.mdpi.com/2227-9059/12/3/696 |
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