Cisplatin-induced azoospermia and testicular damage ameliorated by adipose-derived mesenchymal stem cells
Abstract Background The testes are highly susceptible to the adverse effects of chemotherapy and radiation at all stages of life. Exposure to these threats mainly occurs during cancer treatment and as an occupational hazard in radiation centers. The present study investigated the regenerative abilit...
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| Format: | Article |
| Language: | English |
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BMC
2023-01-01
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| Series: | Biological Research |
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| Online Access: | https://doi.org/10.1186/s40659-022-00410-5 |
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| author | Hamdy Y. Ismail Nora A. Shaker Shaymaa Hussein Adel Tohamy Mohamed Fathi Hamdy Rizk Y. R. Wally |
| author_facet | Hamdy Y. Ismail Nora A. Shaker Shaymaa Hussein Adel Tohamy Mohamed Fathi Hamdy Rizk Y. R. Wally |
| author_sort | Hamdy Y. Ismail |
| collection | DOAJ |
| description | Abstract Background The testes are highly susceptible to the adverse effects of chemotherapy and radiation at all stages of life. Exposure to these threats mainly occurs during cancer treatment and as an occupational hazard in radiation centers. The present study investigated the regenerative ability of adipose-derived mesenchymal stem cells (ADMSCs) against the adverse effects of cisplatin on the structure and function of the testes. Methods New Zealand white rabbits (N = 15) were divided into three groups of five: a negative control group (no treatment), a cisplatin group (single dose of cisplatin into each testis followed three days later by a PBS injection), and a cisplatin + ADMSCs group (cisplatin injection followed three days later by an ADMSC injection). On day 45 post-treatment, serum testosterone levels were evaluated, and the testes and epididymis were collected for histology, oxidative stress examination, and epididymal sperm analysis. Results Cisplatin caused damage to the testicular tissue and decreased serum testosterone levels, epididymal sperm counts, and oxidants. An antioxidant imbalance was detected due to increasing malondialdehyde (MDA) and reduced glutathione (GSH) levels in testicular tissue. The ADMSC-treated group displayed a moderate epididymal sperm count, adequate antioxidant protection, suitable hormone levels, and enhanced testicular tissue morphology. Conclusions ADMSCs treatment repaired damaged testicular tissue, enhanced biochemical parameters, and modified pathological changes caused by cisplatin. |
| first_indexed | 2024-04-10T21:05:55Z |
| format | Article |
| id | doaj.art-e82681757ab044ba92919e18f42368df |
| institution | Directory Open Access Journal |
| issn | 0717-6287 |
| language | English |
| last_indexed | 2024-04-10T21:05:55Z |
| publishDate | 2023-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Research |
| spelling | doaj.art-e82681757ab044ba92919e18f42368df2023-01-22T12:05:18ZengBMCBiological Research0717-62872023-01-0156111410.1186/s40659-022-00410-5Cisplatin-induced azoospermia and testicular damage ameliorated by adipose-derived mesenchymal stem cellsHamdy Y. Ismail0Nora A. Shaker1Shaymaa Hussein2Adel Tohamy3Mohamed Fathi4Hamdy Rizk5Y. R. Wally6Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Cairo UniversityDepartment of Anatomy and Embryology, Faculty of Veterinary Medicine, Cairo UniversityDepartment of Cytology and Histology, Faculty of Veterinary Medicine, Cairo UniversityDepartment of Toxicology & Forensic Medicine, Faculty of Veterinary Medicine, Cairo UniversityDepartment of Theriogenology, Faculty of Veterinary Medicine, Cairo UniversityDepartment of Anatomy and Embryology, Faculty of Veterinary Medicine, Cairo UniversityDepartment of Anatomy and Embryology, Faculty of Veterinary Medicine, Cairo UniversityAbstract Background The testes are highly susceptible to the adverse effects of chemotherapy and radiation at all stages of life. Exposure to these threats mainly occurs during cancer treatment and as an occupational hazard in radiation centers. The present study investigated the regenerative ability of adipose-derived mesenchymal stem cells (ADMSCs) against the adverse effects of cisplatin on the structure and function of the testes. Methods New Zealand white rabbits (N = 15) were divided into three groups of five: a negative control group (no treatment), a cisplatin group (single dose of cisplatin into each testis followed three days later by a PBS injection), and a cisplatin + ADMSCs group (cisplatin injection followed three days later by an ADMSC injection). On day 45 post-treatment, serum testosterone levels were evaluated, and the testes and epididymis were collected for histology, oxidative stress examination, and epididymal sperm analysis. Results Cisplatin caused damage to the testicular tissue and decreased serum testosterone levels, epididymal sperm counts, and oxidants. An antioxidant imbalance was detected due to increasing malondialdehyde (MDA) and reduced glutathione (GSH) levels in testicular tissue. The ADMSC-treated group displayed a moderate epididymal sperm count, adequate antioxidant protection, suitable hormone levels, and enhanced testicular tissue morphology. Conclusions ADMSCs treatment repaired damaged testicular tissue, enhanced biochemical parameters, and modified pathological changes caused by cisplatin.https://doi.org/10.1186/s40659-022-00410-5Testicular degenerationSperm analysisOxidative stressCisplatin side-effects |
| spellingShingle | Hamdy Y. Ismail Nora A. Shaker Shaymaa Hussein Adel Tohamy Mohamed Fathi Hamdy Rizk Y. R. Wally Cisplatin-induced azoospermia and testicular damage ameliorated by adipose-derived mesenchymal stem cells Biological Research Testicular degeneration Sperm analysis Oxidative stress Cisplatin side-effects |
| title | Cisplatin-induced azoospermia and testicular damage ameliorated by adipose-derived mesenchymal stem cells |
| title_full | Cisplatin-induced azoospermia and testicular damage ameliorated by adipose-derived mesenchymal stem cells |
| title_fullStr | Cisplatin-induced azoospermia and testicular damage ameliorated by adipose-derived mesenchymal stem cells |
| title_full_unstemmed | Cisplatin-induced azoospermia and testicular damage ameliorated by adipose-derived mesenchymal stem cells |
| title_short | Cisplatin-induced azoospermia and testicular damage ameliorated by adipose-derived mesenchymal stem cells |
| title_sort | cisplatin induced azoospermia and testicular damage ameliorated by adipose derived mesenchymal stem cells |
| topic | Testicular degeneration Sperm analysis Oxidative stress Cisplatin side-effects |
| url | https://doi.org/10.1186/s40659-022-00410-5 |
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