Sensitivity and specificity of D2-40 in differentiating Kaposi sarcoma from its mimickers
Background Kaposi sarcoma (KS) is a low-grade malignant vascular neoplasm, whose etiology is associated with human herpes virus 8 (HHV8). Owing to its broad clinicopathological spectrum, KS mimics a variety of non-KS vascular lesions both clinically and histologically, thus posing a diagnostic chall...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2021-01-01
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| Series: | Journal of the Egyptian Women’s Dermatologic Society |
| Subjects: | |
| Online Access: | http://www.jewd.eg.net//article.asp?issn=1687-1537;year=2021;volume=18;issue=1;spage=67;epage=74;aulast=Genedy |
| _version_ | 1818354961513185280 |
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| author | Rasha M Genedy Ashraf M Hamza Amany A Abdel Latef Ali Alwi El-Beity |
| author_facet | Rasha M Genedy Ashraf M Hamza Amany A Abdel Latef Ali Alwi El-Beity |
| author_sort | Rasha M Genedy |
| collection | DOAJ |
| description | Background Kaposi sarcoma (KS) is a low-grade malignant vascular neoplasm, whose etiology is associated with human herpes virus 8 (HHV8). Owing to its broad clinicopathological spectrum, KS mimics a variety of non-KS vascular lesions both clinically and histologically, thus posing a diagnostic challenge. Immunohistochemical markers are needed to confirm diagnosis.
Objective To study the sensitivity and specificity of D2-40 in comparison with HHV8-latent nuclear antigen 1 to identify KS among other cutaneous vascular proliferative lesions.
Patients and methods The present study was carried out on 50 cases with cutaneous vascular proliferation. Examination of hematoxylin and eosin-stained sections was done for all cases to verify the diagnosis. Additional sections of all cases were subjected to immunohistochemical stains for the following primary antibodies: HHV8 and D2-40 monoclonal antibody.
Results The study included 19 KS cases and 31 non-KS. Among KS lesions 15.8% were patch stage, 26.3% plaque stage, 36.84% nodular stage, 10.5% pyogenic granuloma-like KS, and 10.5% intravascular KS. All KS cases stained positive for HHV8-latent nuclear antigen 1, whereas none of other vascular lesions showed positive staining. Immunohistochemistry using D2-40 showed positive staining in 70% (n=35) and negative staining in 30% (n=15) of the total cases. All KS lesions expressed D2-40 in both endothelial lining and spindle cells. A total of 16 cases of non-KS vascular lesions expressed D2-40 in endothelial lining only. D2-40 positivity of both endothelial lining and spindle cells was found to be equally sensitive and specific to HHV8 for diagnosis of KS.
Conclusion D2-40 positivity of both vascular lining and spindle cells can be considered both sensitive and specific stain to differentiate KS from other vascular lesions. |
| first_indexed | 2024-12-13T19:33:44Z |
| format | Article |
| id | doaj.art-e826b99a746a4b8a8b7a8d36960ac9ec |
| institution | Directory Open Access Journal |
| issn | 2090-2565 |
| language | English |
| last_indexed | 2024-12-13T19:33:44Z |
| publishDate | 2021-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of the Egyptian Women’s Dermatologic Society |
| spelling | doaj.art-e826b99a746a4b8a8b7a8d36960ac9ec2022-12-21T23:33:52ZengWolters Kluwer Medknow PublicationsJournal of the Egyptian Women’s Dermatologic Society2090-25652021-01-01181677410.4103/jewd.jewd_61_20Sensitivity and specificity of D2-40 in differentiating Kaposi sarcoma from its mimickersRasha M GenedyAshraf M HamzaAmany A Abdel LatefAli Alwi El-BeityBackground Kaposi sarcoma (KS) is a low-grade malignant vascular neoplasm, whose etiology is associated with human herpes virus 8 (HHV8). Owing to its broad clinicopathological spectrum, KS mimics a variety of non-KS vascular lesions both clinically and histologically, thus posing a diagnostic challenge. Immunohistochemical markers are needed to confirm diagnosis. Objective To study the sensitivity and specificity of D2-40 in comparison with HHV8-latent nuclear antigen 1 to identify KS among other cutaneous vascular proliferative lesions. Patients and methods The present study was carried out on 50 cases with cutaneous vascular proliferation. Examination of hematoxylin and eosin-stained sections was done for all cases to verify the diagnosis. Additional sections of all cases were subjected to immunohistochemical stains for the following primary antibodies: HHV8 and D2-40 monoclonal antibody. Results The study included 19 KS cases and 31 non-KS. Among KS lesions 15.8% were patch stage, 26.3% plaque stage, 36.84% nodular stage, 10.5% pyogenic granuloma-like KS, and 10.5% intravascular KS. All KS cases stained positive for HHV8-latent nuclear antigen 1, whereas none of other vascular lesions showed positive staining. Immunohistochemistry using D2-40 showed positive staining in 70% (n=35) and negative staining in 30% (n=15) of the total cases. All KS lesions expressed D2-40 in both endothelial lining and spindle cells. A total of 16 cases of non-KS vascular lesions expressed D2-40 in endothelial lining only. D2-40 positivity of both endothelial lining and spindle cells was found to be equally sensitive and specific to HHV8 for diagnosis of KS. Conclusion D2-40 positivity of both vascular lining and spindle cells can be considered both sensitive and specific stain to differentiate KS from other vascular lesions.http://www.jewd.eg.net//article.asp?issn=1687-1537;year=2021;volume=18;issue=1;spage=67;epage=74;aulast=Genedyd2-40hhv8kaposi sarcoma |
| spellingShingle | Rasha M Genedy Ashraf M Hamza Amany A Abdel Latef Ali Alwi El-Beity Sensitivity and specificity of D2-40 in differentiating Kaposi sarcoma from its mimickers Journal of the Egyptian Women’s Dermatologic Society d2-40 hhv8 kaposi sarcoma |
| title | Sensitivity and specificity of D2-40 in differentiating Kaposi sarcoma from its mimickers |
| title_full | Sensitivity and specificity of D2-40 in differentiating Kaposi sarcoma from its mimickers |
| title_fullStr | Sensitivity and specificity of D2-40 in differentiating Kaposi sarcoma from its mimickers |
| title_full_unstemmed | Sensitivity and specificity of D2-40 in differentiating Kaposi sarcoma from its mimickers |
| title_short | Sensitivity and specificity of D2-40 in differentiating Kaposi sarcoma from its mimickers |
| title_sort | sensitivity and specificity of d2 40 in differentiating kaposi sarcoma from its mimickers |
| topic | d2-40 hhv8 kaposi sarcoma |
| url | http://www.jewd.eg.net//article.asp?issn=1687-1537;year=2021;volume=18;issue=1;spage=67;epage=74;aulast=Genedy |
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