Methyltransferase as Antibiotics Against Foodborne Pathogens: An In Silico Approach for Exploring Enzyme as Enzymobiotics
The development of resistance in microbes against antibiotics and limited choice for the use of chemical preservatives in food lead the urgent need to search for an alternative to antibiotics. The enzymes are catalytic proteins that catalyze digestion of bacterial cell walls and protein requirements...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-01-01
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| Series: | Frontiers in Genetics |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2021.800587/full |
| _version_ | 1818974676476493824 |
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| author | Varish Ahmad Aftab Ahmad Mohammed F. Abuzinadah Salwa Al-Thawdi Ghazala Yunus |
| author_facet | Varish Ahmad Aftab Ahmad Mohammed F. Abuzinadah Salwa Al-Thawdi Ghazala Yunus |
| author_sort | Varish Ahmad |
| collection | DOAJ |
| description | The development of resistance in microbes against antibiotics and limited choice for the use of chemical preservatives in food lead the urgent need to search for an alternative to antibiotics. The enzymes are catalytic proteins that catalyze digestion of bacterial cell walls and protein requirements for the survival of the cell. To study methyltransferase as antibiotics against foodborne pathogen, the methyltransferase enzyme sequence was modeled and its interactions were analyzed against a membrane protein of the gram-positive and gram-negative bacteria through in silico protein–protein interactions. The methyltransferase interaction with cellular protein was found to be maximum, due to the maximum PatchDock Score (15808), which was followed by colicin (12864) and amoxicillin (4122). The modeled protein has found to be interact more significantly to inhibit the indicator bacteria than the tested antibiotics and antimicrobial colicin protein. Thus, model enzyme methyltransferase could be used as enzymobiotics. Moreover, peptide sequences similar to this enzyme sequence need to be designed and evaluated against the microbial pathogen. |
| first_indexed | 2024-12-20T15:43:51Z |
| format | Article |
| id | doaj.art-e82b5cac674746b6951e2f0ccac80ba2 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-20T15:43:51Z |
| publishDate | 2022-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-e82b5cac674746b6951e2f0ccac80ba22022-12-21T19:35:04ZengFrontiers Media S.A.Frontiers in Genetics1664-80212022-01-011210.3389/fgene.2021.800587800587Methyltransferase as Antibiotics Against Foodborne Pathogens: An In Silico Approach for Exploring Enzyme as EnzymobioticsVarish Ahmad0Aftab Ahmad1Mohammed F. Abuzinadah2Salwa Al-Thawdi3Ghazala Yunus4Health Information Technology Department, Faculty of Applied Studies, King Abdulaziz University, Jeddah, Saudi ArabiaHealth Information Technology Department, Faculty of Applied Studies, King Abdulaziz University, Jeddah, Saudi ArabiaDepartment of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi ArabiaDepartment of Biology, College of Science, University of Bahrain, Sakhir, BahrainDepartment of Basic Science, University of Hail, Hail, Saudi ArabiaThe development of resistance in microbes against antibiotics and limited choice for the use of chemical preservatives in food lead the urgent need to search for an alternative to antibiotics. The enzymes are catalytic proteins that catalyze digestion of bacterial cell walls and protein requirements for the survival of the cell. To study methyltransferase as antibiotics against foodborne pathogen, the methyltransferase enzyme sequence was modeled and its interactions were analyzed against a membrane protein of the gram-positive and gram-negative bacteria through in silico protein–protein interactions. The methyltransferase interaction with cellular protein was found to be maximum, due to the maximum PatchDock Score (15808), which was followed by colicin (12864) and amoxicillin (4122). The modeled protein has found to be interact more significantly to inhibit the indicator bacteria than the tested antibiotics and antimicrobial colicin protein. Thus, model enzyme methyltransferase could be used as enzymobiotics. Moreover, peptide sequences similar to this enzyme sequence need to be designed and evaluated against the microbial pathogen.https://www.frontiersin.org/articles/10.3389/fgene.2021.800587/fullmethyltransferaseantimicrobialdrug resistantprotein–protein interactionenzymobiotics |
| spellingShingle | Varish Ahmad Aftab Ahmad Mohammed F. Abuzinadah Salwa Al-Thawdi Ghazala Yunus Methyltransferase as Antibiotics Against Foodborne Pathogens: An In Silico Approach for Exploring Enzyme as Enzymobiotics Frontiers in Genetics methyltransferase antimicrobial drug resistant protein–protein interaction enzymobiotics |
| title | Methyltransferase as Antibiotics Against Foodborne Pathogens: An In Silico Approach for Exploring Enzyme as Enzymobiotics |
| title_full | Methyltransferase as Antibiotics Against Foodborne Pathogens: An In Silico Approach for Exploring Enzyme as Enzymobiotics |
| title_fullStr | Methyltransferase as Antibiotics Against Foodborne Pathogens: An In Silico Approach for Exploring Enzyme as Enzymobiotics |
| title_full_unstemmed | Methyltransferase as Antibiotics Against Foodborne Pathogens: An In Silico Approach for Exploring Enzyme as Enzymobiotics |
| title_short | Methyltransferase as Antibiotics Against Foodborne Pathogens: An In Silico Approach for Exploring Enzyme as Enzymobiotics |
| title_sort | methyltransferase as antibiotics against foodborne pathogens an in silico approach for exploring enzyme as enzymobiotics |
| topic | methyltransferase antimicrobial drug resistant protein–protein interaction enzymobiotics |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2021.800587/full |
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