Cytogenetics of four foam-nesting frog species of the Physalaemus gracilis group (Anura, Leptodactylidae)

Abstract Intending to increase the knowledge about cytogenetics of Physalaemus and the sparsely studied P. gracilis group, we analyzed the karyotypes of P. carrizorum, P. gracilis, P. lisei, and P. sp. aff. gracilis. We studied chromosome morphology, heterochromatin patterns, Ag-NORs location and ma...

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Main Authors: JUAN M. FERRO, ALBERTO TAFFAREL, CRISTIAN TOMATIS, CLAUDIO BORTEIRO, FRANCISCO KOLENC, KALEB P. GATTO, LUCIANA B. LOURENÇO, DIEGO BALDO
Format: Article
Language:English
Published: Academia Brasileira de Ciências 2021-04-01
Series:Anais da Academia Brasileira de Ciências
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652022000101314&lng=en&tlng=en
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author JUAN M. FERRO
ALBERTO TAFFAREL
CRISTIAN TOMATIS
CLAUDIO BORTEIRO
FRANCISCO KOLENC
KALEB P. GATTO
LUCIANA B. LOURENÇO
DIEGO BALDO
author_facet JUAN M. FERRO
ALBERTO TAFFAREL
CRISTIAN TOMATIS
CLAUDIO BORTEIRO
FRANCISCO KOLENC
KALEB P. GATTO
LUCIANA B. LOURENÇO
DIEGO BALDO
author_sort JUAN M. FERRO
collection DOAJ
description Abstract Intending to increase the knowledge about cytogenetics of Physalaemus and the sparsely studied P. gracilis group, we analyzed the karyotypes of P. carrizorum, P. gracilis, P. lisei, and P. sp. aff. gracilis. We studied chromosome morphology, heterochromatin patterns, Ag-NORs location and mapped the repetitive DNA sequence PcP190. All species showed diploid karyotypes composed of 22 bi-armed chromosomes and similar C- bands and Ag-NOR patterns. C-bands were mainly centromeric and pericentromeric; non-centromeric C-bands were detected on the telomeres of pair 1 in P. lisei, although polymorphic, and interstitially on pair 10 of P. gracilis. This last character is useful to distinguish P. gracilis from its sibling species P. sp. aff. gracilis. The Ag-NOR sites were detected on the long arms of chromosome pair 8 but with a variable position among species. Clusters of PcP190 showed centromeric and pericentromeric positions coincident with conspicuous C-bands, on pairs 2 and 9 in P. gracilis and P. sp. aff. gracilis, pair 3 in P. carrizorum, and pair 7 in P. lisei. These results significantly increase the knowledge about Physalaemus cytogenetics and encourage further studies on the satellite PcP190 in other genera of Leiuperinae to better understand its taxonomic distribution and the evolutionary dynamics.
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spelling doaj.art-e82baf3bfd634cbcab5a7fa828ecf0b52022-12-21T19:06:57ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902021-04-0194110.1590/0001-3765202220200092Cytogenetics of four foam-nesting frog species of the Physalaemus gracilis group (Anura, Leptodactylidae)JUAN M. FERROhttps://orcid.org/0000-0003-0287-5567ALBERTO TAFFARELhttps://orcid.org/0000-0002-9479-5152CRISTIAN TOMATIShttps://orcid.org/0000-0001-7860-7951CLAUDIO BORTEIROhttps://orcid.org/0000-0002-8722-0334FRANCISCO KOLENChttps://orcid.org/0000-0002-9985-3630KALEB P. GATTOhttps://orcid.org/0000-0002-2261-229XLUCIANA B. LOURENÇOhttps://orcid.org/0000-0001-6602-6850DIEGO BALDOhttps://orcid.org/0000-0003-2382-0872Abstract Intending to increase the knowledge about cytogenetics of Physalaemus and the sparsely studied P. gracilis group, we analyzed the karyotypes of P. carrizorum, P. gracilis, P. lisei, and P. sp. aff. gracilis. We studied chromosome morphology, heterochromatin patterns, Ag-NORs location and mapped the repetitive DNA sequence PcP190. All species showed diploid karyotypes composed of 22 bi-armed chromosomes and similar C- bands and Ag-NOR patterns. C-bands were mainly centromeric and pericentromeric; non-centromeric C-bands were detected on the telomeres of pair 1 in P. lisei, although polymorphic, and interstitially on pair 10 of P. gracilis. This last character is useful to distinguish P. gracilis from its sibling species P. sp. aff. gracilis. The Ag-NOR sites were detected on the long arms of chromosome pair 8 but with a variable position among species. Clusters of PcP190 showed centromeric and pericentromeric positions coincident with conspicuous C-bands, on pairs 2 and 9 in P. gracilis and P. sp. aff. gracilis, pair 3 in P. carrizorum, and pair 7 in P. lisei. These results significantly increase the knowledge about Physalaemus cytogenetics and encourage further studies on the satellite PcP190 in other genera of Leiuperinae to better understand its taxonomic distribution and the evolutionary dynamics.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652022000101314&lng=en&tlng=enLeiuperinaeAg-NORsC-BandssatDNAPcP190
spellingShingle JUAN M. FERRO
ALBERTO TAFFAREL
CRISTIAN TOMATIS
CLAUDIO BORTEIRO
FRANCISCO KOLENC
KALEB P. GATTO
LUCIANA B. LOURENÇO
DIEGO BALDO
Cytogenetics of four foam-nesting frog species of the Physalaemus gracilis group (Anura, Leptodactylidae)
Anais da Academia Brasileira de Ciências
Leiuperinae
Ag-NORs
C-Bands
satDNA
PcP190
title Cytogenetics of four foam-nesting frog species of the Physalaemus gracilis group (Anura, Leptodactylidae)
title_full Cytogenetics of four foam-nesting frog species of the Physalaemus gracilis group (Anura, Leptodactylidae)
title_fullStr Cytogenetics of four foam-nesting frog species of the Physalaemus gracilis group (Anura, Leptodactylidae)
title_full_unstemmed Cytogenetics of four foam-nesting frog species of the Physalaemus gracilis group (Anura, Leptodactylidae)
title_short Cytogenetics of four foam-nesting frog species of the Physalaemus gracilis group (Anura, Leptodactylidae)
title_sort cytogenetics of four foam nesting frog species of the physalaemus gracilis group anura leptodactylidae
topic Leiuperinae
Ag-NORs
C-Bands
satDNA
PcP190
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652022000101314&lng=en&tlng=en
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