Cytogenetics of four foam-nesting frog species of the Physalaemus gracilis group (Anura, Leptodactylidae)
Abstract Intending to increase the knowledge about cytogenetics of Physalaemus and the sparsely studied P. gracilis group, we analyzed the karyotypes of P. carrizorum, P. gracilis, P. lisei, and P. sp. aff. gracilis. We studied chromosome morphology, heterochromatin patterns, Ag-NORs location and ma...
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Academia Brasileira de Ciências
2021-04-01
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Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652022000101314&lng=en&tlng=en |
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author | JUAN M. FERRO ALBERTO TAFFAREL CRISTIAN TOMATIS CLAUDIO BORTEIRO FRANCISCO KOLENC KALEB P. GATTO LUCIANA B. LOURENÇO DIEGO BALDO |
author_facet | JUAN M. FERRO ALBERTO TAFFAREL CRISTIAN TOMATIS CLAUDIO BORTEIRO FRANCISCO KOLENC KALEB P. GATTO LUCIANA B. LOURENÇO DIEGO BALDO |
author_sort | JUAN M. FERRO |
collection | DOAJ |
description | Abstract Intending to increase the knowledge about cytogenetics of Physalaemus and the sparsely studied P. gracilis group, we analyzed the karyotypes of P. carrizorum, P. gracilis, P. lisei, and P. sp. aff. gracilis. We studied chromosome morphology, heterochromatin patterns, Ag-NORs location and mapped the repetitive DNA sequence PcP190. All species showed diploid karyotypes composed of 22 bi-armed chromosomes and similar C- bands and Ag-NOR patterns. C-bands were mainly centromeric and pericentromeric; non-centromeric C-bands were detected on the telomeres of pair 1 in P. lisei, although polymorphic, and interstitially on pair 10 of P. gracilis. This last character is useful to distinguish P. gracilis from its sibling species P. sp. aff. gracilis. The Ag-NOR sites were detected on the long arms of chromosome pair 8 but with a variable position among species. Clusters of PcP190 showed centromeric and pericentromeric positions coincident with conspicuous C-bands, on pairs 2 and 9 in P. gracilis and P. sp. aff. gracilis, pair 3 in P. carrizorum, and pair 7 in P. lisei. These results significantly increase the knowledge about Physalaemus cytogenetics and encourage further studies on the satellite PcP190 in other genera of Leiuperinae to better understand its taxonomic distribution and the evolutionary dynamics. |
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issn | 1678-2690 |
language | English |
last_indexed | 2024-12-21T10:40:04Z |
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series | Anais da Academia Brasileira de Ciências |
spelling | doaj.art-e82baf3bfd634cbcab5a7fa828ecf0b52022-12-21T19:06:57ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902021-04-0194110.1590/0001-3765202220200092Cytogenetics of four foam-nesting frog species of the Physalaemus gracilis group (Anura, Leptodactylidae)JUAN M. FERROhttps://orcid.org/0000-0003-0287-5567ALBERTO TAFFARELhttps://orcid.org/0000-0002-9479-5152CRISTIAN TOMATIShttps://orcid.org/0000-0001-7860-7951CLAUDIO BORTEIROhttps://orcid.org/0000-0002-8722-0334FRANCISCO KOLENChttps://orcid.org/0000-0002-9985-3630KALEB P. GATTOhttps://orcid.org/0000-0002-2261-229XLUCIANA B. LOURENÇOhttps://orcid.org/0000-0001-6602-6850DIEGO BALDOhttps://orcid.org/0000-0003-2382-0872Abstract Intending to increase the knowledge about cytogenetics of Physalaemus and the sparsely studied P. gracilis group, we analyzed the karyotypes of P. carrizorum, P. gracilis, P. lisei, and P. sp. aff. gracilis. We studied chromosome morphology, heterochromatin patterns, Ag-NORs location and mapped the repetitive DNA sequence PcP190. All species showed diploid karyotypes composed of 22 bi-armed chromosomes and similar C- bands and Ag-NOR patterns. C-bands were mainly centromeric and pericentromeric; non-centromeric C-bands were detected on the telomeres of pair 1 in P. lisei, although polymorphic, and interstitially on pair 10 of P. gracilis. This last character is useful to distinguish P. gracilis from its sibling species P. sp. aff. gracilis. The Ag-NOR sites were detected on the long arms of chromosome pair 8 but with a variable position among species. Clusters of PcP190 showed centromeric and pericentromeric positions coincident with conspicuous C-bands, on pairs 2 and 9 in P. gracilis and P. sp. aff. gracilis, pair 3 in P. carrizorum, and pair 7 in P. lisei. These results significantly increase the knowledge about Physalaemus cytogenetics and encourage further studies on the satellite PcP190 in other genera of Leiuperinae to better understand its taxonomic distribution and the evolutionary dynamics.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652022000101314&lng=en&tlng=enLeiuperinaeAg-NORsC-BandssatDNAPcP190 |
spellingShingle | JUAN M. FERRO ALBERTO TAFFAREL CRISTIAN TOMATIS CLAUDIO BORTEIRO FRANCISCO KOLENC KALEB P. GATTO LUCIANA B. LOURENÇO DIEGO BALDO Cytogenetics of four foam-nesting frog species of the Physalaemus gracilis group (Anura, Leptodactylidae) Anais da Academia Brasileira de Ciências Leiuperinae Ag-NORs C-Bands satDNA PcP190 |
title | Cytogenetics of four foam-nesting frog species of the Physalaemus gracilis group (Anura, Leptodactylidae) |
title_full | Cytogenetics of four foam-nesting frog species of the Physalaemus gracilis group (Anura, Leptodactylidae) |
title_fullStr | Cytogenetics of four foam-nesting frog species of the Physalaemus gracilis group (Anura, Leptodactylidae) |
title_full_unstemmed | Cytogenetics of four foam-nesting frog species of the Physalaemus gracilis group (Anura, Leptodactylidae) |
title_short | Cytogenetics of four foam-nesting frog species of the Physalaemus gracilis group (Anura, Leptodactylidae) |
title_sort | cytogenetics of four foam nesting frog species of the physalaemus gracilis group anura leptodactylidae |
topic | Leiuperinae Ag-NORs C-Bands satDNA PcP190 |
url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652022000101314&lng=en&tlng=en |
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