DNA Methylome Distinguishes Head and Neck Cancer from Potentially Malignant Oral Lesions and Healthy Oral Mucosa
There is a strong need to find new, good biomarkers of head and neck squamous cell carcinoma (HNSCC) because of the bad prognoses and high mortality rates. The aim of this study was to identify the potential biomarkers in HNSCC that have differences in their DNA methylome and potentially premalignan...
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MDPI AG
2020-09-01
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| Series: | International Journal of Molecular Sciences |
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| author | Nina Milutin Gašperov Ivan Sabol Ksenija Božinović Emil Dediol Marinka Mravak-Stipetić Danilo Licastro Simeone Dal Monego Magdalena Grce |
| author_facet | Nina Milutin Gašperov Ivan Sabol Ksenija Božinović Emil Dediol Marinka Mravak-Stipetić Danilo Licastro Simeone Dal Monego Magdalena Grce |
| author_sort | Nina Milutin Gašperov |
| collection | DOAJ |
| description | There is a strong need to find new, good biomarkers of head and neck squamous cell carcinoma (HNSCC) because of the bad prognoses and high mortality rates. The aim of this study was to identify the potential biomarkers in HNSCC that have differences in their DNA methylome and potentially premalignant oral lesions, in comparison to healthy oral mucosa. In this study, 32 oral samples were tested: nine healthy oral mucosae, 13 HNSCC, and 10 oral lesions for DNA methylation by the Infinium MethylationEPIC BeadChip. Our findings showed that a panel of genes significantly hypermethylated in their promoters or specific sites in HNSCC samples in comparison to healthy oral samples, which are mainly oncogenes, receptor, and transcription factor genes, or genes included in cell cycle, transformation, apoptosis, and autophagy. A group of hypomethylated genes in HNSCC, in comparison to healthy oral mucosa, are mainly involved in the host immune response and transcriptional regulation. The results also showed significant differences in gene methylation between HNSCC and potentially premalignant oral lesions, as well as differently methylated genes that discriminate between oral lesions and healthy mucosa. The given methylation panels point to novel potential biomarkers for early diagnostics of HNSCC, as well as potentially premalignant oral lesions. |
| first_indexed | 2024-03-10T16:14:04Z |
| format | Article |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T16:14:04Z |
| publishDate | 2020-09-01 |
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| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-e82c72f28a1248ad9ace1129256e60be2023-11-20T14:13:49ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-09-012118685310.3390/ijms21186853DNA Methylome Distinguishes Head and Neck Cancer from Potentially Malignant Oral Lesions and Healthy Oral MucosaNina Milutin Gašperov0Ivan Sabol1Ksenija Božinović2Emil Dediol3Marinka Mravak-Stipetić4Danilo Licastro5Simeone Dal Monego6Magdalena Grce7Division of Molecular Medicine, Ruđer Bošković Institute, 10000 Zagreb, CroatiaDivision of Molecular Medicine, Ruđer Bošković Institute, 10000 Zagreb, CroatiaDivision of Molecular Medicine, Ruđer Bošković Institute, 10000 Zagreb, CroatiaDepartment of Maxillofacial Surgery, School of Medicine, Clinical Hospital Dubrava, University of Zagreb, 10000 Zagreb, CroatiaDepartment of Oral Medicine, School of Dental Medicine, University of Zagreb, 10000 Zagreb, CroatiaARGO Open Lab Platform for Genome sequencing, AREA Science Park, Padriciano, 99, 34149 Trieste, ItalyARGO Open Lab Platform for Genome sequencing, AREA Science Park, Padriciano, 99, 34149 Trieste, ItalyDivision of Molecular Medicine, Ruđer Bošković Institute, 10000 Zagreb, CroatiaThere is a strong need to find new, good biomarkers of head and neck squamous cell carcinoma (HNSCC) because of the bad prognoses and high mortality rates. The aim of this study was to identify the potential biomarkers in HNSCC that have differences in their DNA methylome and potentially premalignant oral lesions, in comparison to healthy oral mucosa. In this study, 32 oral samples were tested: nine healthy oral mucosae, 13 HNSCC, and 10 oral lesions for DNA methylation by the Infinium MethylationEPIC BeadChip. Our findings showed that a panel of genes significantly hypermethylated in their promoters or specific sites in HNSCC samples in comparison to healthy oral samples, which are mainly oncogenes, receptor, and transcription factor genes, or genes included in cell cycle, transformation, apoptosis, and autophagy. A group of hypomethylated genes in HNSCC, in comparison to healthy oral mucosa, are mainly involved in the host immune response and transcriptional regulation. The results also showed significant differences in gene methylation between HNSCC and potentially premalignant oral lesions, as well as differently methylated genes that discriminate between oral lesions and healthy mucosa. The given methylation panels point to novel potential biomarkers for early diagnostics of HNSCC, as well as potentially premalignant oral lesions.https://www.mdpi.com/1422-0067/21/18/6853DNA methylationhead and neck squamous cell carcinoma (HNSCC)potentially premalignant oral lesionshealthy oral mucosahuman papillomavirus (HPV) |
| spellingShingle | Nina Milutin Gašperov Ivan Sabol Ksenija Božinović Emil Dediol Marinka Mravak-Stipetić Danilo Licastro Simeone Dal Monego Magdalena Grce DNA Methylome Distinguishes Head and Neck Cancer from Potentially Malignant Oral Lesions and Healthy Oral Mucosa International Journal of Molecular Sciences DNA methylation head and neck squamous cell carcinoma (HNSCC) potentially premalignant oral lesions healthy oral mucosa human papillomavirus (HPV) |
| title | DNA Methylome Distinguishes Head and Neck Cancer from Potentially Malignant Oral Lesions and Healthy Oral Mucosa |
| title_full | DNA Methylome Distinguishes Head and Neck Cancer from Potentially Malignant Oral Lesions and Healthy Oral Mucosa |
| title_fullStr | DNA Methylome Distinguishes Head and Neck Cancer from Potentially Malignant Oral Lesions and Healthy Oral Mucosa |
| title_full_unstemmed | DNA Methylome Distinguishes Head and Neck Cancer from Potentially Malignant Oral Lesions and Healthy Oral Mucosa |
| title_short | DNA Methylome Distinguishes Head and Neck Cancer from Potentially Malignant Oral Lesions and Healthy Oral Mucosa |
| title_sort | dna methylome distinguishes head and neck cancer from potentially malignant oral lesions and healthy oral mucosa |
| topic | DNA methylation head and neck squamous cell carcinoma (HNSCC) potentially premalignant oral lesions healthy oral mucosa human papillomavirus (HPV) |
| url | https://www.mdpi.com/1422-0067/21/18/6853 |
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