Glucose Conversion for Biobutanol Production from Fresh <i>Chlorella sorokiniana</i> via Direct Enzymatic Hydrolysis
Microalgae, which accumulate considerable carbohydrates, are a potential source of glucose for biofuel fermentation. In this study, we investigated the enzymatic hydrolysis efficiency of wet microalgal biomass compared with freeze-dried and oven-dried biomasses, both with and without an acidic pretr...
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| Format: | Article |
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MDPI AG
2023-03-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/9/3/284 |
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| author | Jinzhi Yang Di Cai Xudong Liu Liqi Zhu Changwei Zhang Qing Peng Yanxia Han Guozhen Liu Ming Yang |
| author_facet | Jinzhi Yang Di Cai Xudong Liu Liqi Zhu Changwei Zhang Qing Peng Yanxia Han Guozhen Liu Ming Yang |
| author_sort | Jinzhi Yang |
| collection | DOAJ |
| description | Microalgae, which accumulate considerable carbohydrates, are a potential source of glucose for biofuel fermentation. In this study, we investigated the enzymatic hydrolysis efficiency of wet microalgal biomass compared with freeze-dried and oven-dried biomasses, both with and without an acidic pretreatment. With the dilute sulfuric acid pretreatment followed by amy (α-amylase and amyloglucosidase) and cellulase hydrolysis, approximately 95.4% of the glucose was recovered; however, 88.5% was released by the pretreatment with 2% (<i>w/v</i>) sulfuric acid, which indicates the potential of the acids for direct saccharification process. There were no considerable differences in the glucose yields among the three kinds of materials. In the direct amy hydrolysis without any pretreatment, a 78.7% glucose yield was obtained, and the addition of cellulase had no significant effect on the hydrolysis to glucose. Compared with the oven-dried biomass, the wet biomass produced a substantially higher glucose yield, which is possibly because the cross-linked cells of the oven-dried biomass prevented the accessibility of the enzymes. According to the results, the fresh microalgal biomass without cell disruption can be directly used for enzymatic hydrolysis to produce glucose. The enzymatic hydrolysate of the wet microalgal biomass was successfully used for acetone–butanol–ethanol (ABE) fermentation, which produced 7.2 g/L of ABE, indicating the application potential of wet microalgae in the bioalcohol fuel fermentation process. |
| first_indexed | 2024-03-11T06:34:25Z |
| format | Article |
| id | doaj.art-e82cad78ade041259b5c8bed00f4bd9f |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-11T06:34:25Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-e82cad78ade041259b5c8bed00f4bd9f2023-11-17T11:02:32ZengMDPI AGFermentation2311-56372023-03-019328410.3390/fermentation9030284Glucose Conversion for Biobutanol Production from Fresh <i>Chlorella sorokiniana</i> via Direct Enzymatic HydrolysisJinzhi Yang0Di Cai1Xudong Liu2Liqi Zhu3Changwei Zhang4Qing Peng5Yanxia Han6Guozhen Liu7Ming Yang8College of Life Sciences, Hebei Agricultural University, Baoding 071001, ChinaCollege of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, ChinaCollege of Life Sciences, Hebei Agricultural University, Baoding 071001, ChinaCollege of Life Sciences, Hebei Agricultural University, Baoding 071001, ChinaCollege of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, ChinaCollege of Life Sciences, Hebei Agricultural University, Baoding 071001, ChinaCollege of Life Sciences, Hebei Agricultural University, Baoding 071001, ChinaCollege of Life Sciences, Hebei Agricultural University, Baoding 071001, ChinaCollege of Life Sciences, Hebei Agricultural University, Baoding 071001, ChinaMicroalgae, which accumulate considerable carbohydrates, are a potential source of glucose for biofuel fermentation. In this study, we investigated the enzymatic hydrolysis efficiency of wet microalgal biomass compared with freeze-dried and oven-dried biomasses, both with and without an acidic pretreatment. With the dilute sulfuric acid pretreatment followed by amy (α-amylase and amyloglucosidase) and cellulase hydrolysis, approximately 95.4% of the glucose was recovered; however, 88.5% was released by the pretreatment with 2% (<i>w/v</i>) sulfuric acid, which indicates the potential of the acids for direct saccharification process. There were no considerable differences in the glucose yields among the three kinds of materials. In the direct amy hydrolysis without any pretreatment, a 78.7% glucose yield was obtained, and the addition of cellulase had no significant effect on the hydrolysis to glucose. Compared with the oven-dried biomass, the wet biomass produced a substantially higher glucose yield, which is possibly because the cross-linked cells of the oven-dried biomass prevented the accessibility of the enzymes. According to the results, the fresh microalgal biomass without cell disruption can be directly used for enzymatic hydrolysis to produce glucose. The enzymatic hydrolysate of the wet microalgal biomass was successfully used for acetone–butanol–ethanol (ABE) fermentation, which produced 7.2 g/L of ABE, indicating the application potential of wet microalgae in the bioalcohol fuel fermentation process.https://www.mdpi.com/2311-5637/9/3/284wet microalgaepretreatmentenzymatic hydrolysisglucose |
| spellingShingle | Jinzhi Yang Di Cai Xudong Liu Liqi Zhu Changwei Zhang Qing Peng Yanxia Han Guozhen Liu Ming Yang Glucose Conversion for Biobutanol Production from Fresh <i>Chlorella sorokiniana</i> via Direct Enzymatic Hydrolysis Fermentation wet microalgae pretreatment enzymatic hydrolysis glucose |
| title | Glucose Conversion for Biobutanol Production from Fresh <i>Chlorella sorokiniana</i> via Direct Enzymatic Hydrolysis |
| title_full | Glucose Conversion for Biobutanol Production from Fresh <i>Chlorella sorokiniana</i> via Direct Enzymatic Hydrolysis |
| title_fullStr | Glucose Conversion for Biobutanol Production from Fresh <i>Chlorella sorokiniana</i> via Direct Enzymatic Hydrolysis |
| title_full_unstemmed | Glucose Conversion for Biobutanol Production from Fresh <i>Chlorella sorokiniana</i> via Direct Enzymatic Hydrolysis |
| title_short | Glucose Conversion for Biobutanol Production from Fresh <i>Chlorella sorokiniana</i> via Direct Enzymatic Hydrolysis |
| title_sort | glucose conversion for biobutanol production from fresh i chlorella sorokiniana i via direct enzymatic hydrolysis |
| topic | wet microalgae pretreatment enzymatic hydrolysis glucose |
| url | https://www.mdpi.com/2311-5637/9/3/284 |
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