Oxidative Stress Induced by Selenium Deficiency Contributes to Inflammation, Apoptosis and Necroptosis in the Lungs of Calves
Selenium is an essential trace element for health that can only be obtained through food. However, the pathological processes of selenium deficiency in cattle have received little attention. This study investigated the effects of selenium deficiency on oxidative stress, apoptosis, inflammation, and...
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MDPI AG
2023-03-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/4/796 |
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| author | Jing Mu Lei Lei Yingce Zheng Jia Liu Jie Li Ding Li Guanbo Wang Yun Liu |
| author_facet | Jing Mu Lei Lei Yingce Zheng Jia Liu Jie Li Ding Li Guanbo Wang Yun Liu |
| author_sort | Jing Mu |
| collection | DOAJ |
| description | Selenium is an essential trace element for health that can only be obtained through food. However, the pathological processes of selenium deficiency in cattle have received little attention. This study investigated the effects of selenium deficiency on oxidative stress, apoptosis, inflammation, and necroptosis in the lungs of weaning calves compared with healthy calves as controls. The lung selenium content and the expression of 11 selenoproteins mRNA in selenium-deficient calves were substantially reduced compared with the controls. Pathological results showed engorged alveolar capillaries, thickened alveolar septa, and diffuse interstitial inflammation throughout the alveolar septa. The levels of GSH and T-AOC, as well as the CAT, SOD, and TrxR activities, were significantly decreased compared with healthy calves. MDA and H<sub>2</sub>O<sub>2</sub> were significantly elevated. Meanwhile, the apoptosis activation in the Se-D group was validated. Next, in the Se-D group, several pro-inflammatory cytokines showed higher expression. Further research revealed that the lungs in the Se-D group experienced inflammation via hyperactive NF-κB and MAPK pathways. The high level of expression of <i>c-FLIP</i>, <i>MLKL</i>, <i>RIPK1</i>, and <i>RIPK3</i> indicated that necroptosis also causes lung damage during selenium deficiency. |
| first_indexed | 2024-03-11T05:18:26Z |
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| id | doaj.art-e82fe1f0aea840948f18284036d54ff6 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-11T05:18:26Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-e82fe1f0aea840948f18284036d54ff62023-11-17T18:04:23ZengMDPI AGAntioxidants2076-39212023-03-0112479610.3390/antiox12040796Oxidative Stress Induced by Selenium Deficiency Contributes to Inflammation, Apoptosis and Necroptosis in the Lungs of CalvesJing Mu0Lei Lei1Yingce Zheng2Jia Liu3Jie Li4Ding Li5Guanbo Wang6Yun Liu7Key Laboratory of Comparative Medicine, Department of Veterinary Surgery, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Comparative Medicine, Department of Veterinary Surgery, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaCollege of Life Science, Northeast Agricultural University, Harbin 150030, ChinaVeterinary Medical Teaching Hospital, Northeast Agricultural University, Harbin 150038, ChinaKey Laboratory of Comparative Medicine, Department of Veterinary Surgery, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Comparative Medicine, Department of Veterinary Surgery, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaWellcome-Wolfson Institute for Experimental Medicine, Queen’s University Belfast, Belfast BT7 1NN, UKKey Laboratory of Comparative Medicine, Department of Veterinary Surgery, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaSelenium is an essential trace element for health that can only be obtained through food. However, the pathological processes of selenium deficiency in cattle have received little attention. This study investigated the effects of selenium deficiency on oxidative stress, apoptosis, inflammation, and necroptosis in the lungs of weaning calves compared with healthy calves as controls. The lung selenium content and the expression of 11 selenoproteins mRNA in selenium-deficient calves were substantially reduced compared with the controls. Pathological results showed engorged alveolar capillaries, thickened alveolar septa, and diffuse interstitial inflammation throughout the alveolar septa. The levels of GSH and T-AOC, as well as the CAT, SOD, and TrxR activities, were significantly decreased compared with healthy calves. MDA and H<sub>2</sub>O<sub>2</sub> were significantly elevated. Meanwhile, the apoptosis activation in the Se-D group was validated. Next, in the Se-D group, several pro-inflammatory cytokines showed higher expression. Further research revealed that the lungs in the Se-D group experienced inflammation via hyperactive NF-κB and MAPK pathways. The high level of expression of <i>c-FLIP</i>, <i>MLKL</i>, <i>RIPK1</i>, and <i>RIPK3</i> indicated that necroptosis also causes lung damage during selenium deficiency.https://www.mdpi.com/2076-3921/12/4/796calvesselenium deficiencylung injuryoxidative stresspathomechanism |
| spellingShingle | Jing Mu Lei Lei Yingce Zheng Jia Liu Jie Li Ding Li Guanbo Wang Yun Liu Oxidative Stress Induced by Selenium Deficiency Contributes to Inflammation, Apoptosis and Necroptosis in the Lungs of Calves Antioxidants calves selenium deficiency lung injury oxidative stress pathomechanism |
| title | Oxidative Stress Induced by Selenium Deficiency Contributes to Inflammation, Apoptosis and Necroptosis in the Lungs of Calves |
| title_full | Oxidative Stress Induced by Selenium Deficiency Contributes to Inflammation, Apoptosis and Necroptosis in the Lungs of Calves |
| title_fullStr | Oxidative Stress Induced by Selenium Deficiency Contributes to Inflammation, Apoptosis and Necroptosis in the Lungs of Calves |
| title_full_unstemmed | Oxidative Stress Induced by Selenium Deficiency Contributes to Inflammation, Apoptosis and Necroptosis in the Lungs of Calves |
| title_short | Oxidative Stress Induced by Selenium Deficiency Contributes to Inflammation, Apoptosis and Necroptosis in the Lungs of Calves |
| title_sort | oxidative stress induced by selenium deficiency contributes to inflammation apoptosis and necroptosis in the lungs of calves |
| topic | calves selenium deficiency lung injury oxidative stress pathomechanism |
| url | https://www.mdpi.com/2076-3921/12/4/796 |
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