Assessment of acute and sub-acute toxicity of olive pomace in female Wistar rats
Objective: Olive Pomace (OP) is considered to be a rich source of phenolic compounds. Recently many researches showed a broad biological activity of this by-product of the olive oil production process in addition to their emergence as value-added materials with potential applications in the pharmace...
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| Format: | Article |
| Language: | English |
| Published: |
Verduci Editore
2022-07-01
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| Series: | World Cancer Research Journal |
| Subjects: | |
| Online Access: | https://www.wcrj.net/wp-content/uploads/sites/5/2022/07/e2359.pdf |
| _version_ | 1811198825027600384 |
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| author | Z. Badi B. Guermouche N. Haddam N. Belyagoubi K. Rouigueb H. Benzerjeb M. Dali-Sahi Y. Kachekouche H. Merzouk |
| author_facet | Z. Badi B. Guermouche N. Haddam N. Belyagoubi K. Rouigueb H. Benzerjeb M. Dali-Sahi Y. Kachekouche H. Merzouk |
| author_sort | Z. Badi |
| collection | DOAJ |
| description | Objective: Olive Pomace (OP) is considered to be a rich source of phenolic compounds. Recently many researches showed a broad biological activity of this by-product of the olive oil production process in addition to their emergence as value-added materials with potential applications in the pharmaceutical, food, and nutraceutical industries. The present study is aimed to evaluate in vivo toxicological activities of OP.
Materials and Methods: The qualitative phytochemical analysis aims to determine the key phytoconstituents found in OP. For the in vivo study, two types of tests are performed: acute and 28-day repeated oral toxicity studies in Wistar rats for evaluation of hematological, biochemical, and histological parameters.
Results: The qualitative phytochemical analysis has revealed the presence of polyphenols, flavonoids, tannins, saponins, quinones, anthraquinones, terpenoids, and compounds reduced in our methanol extract of OP. In acute oral toxicity, no treatment-related death or toxic signs are observed in female rats for 14 days in 200, 2000, 3000, and 5000 mg/kg doses, besides LD50 value is found to be up to 2000 mg/kg bodyweight. As for the Globally Harmonized System of Classification and Labelling of Chemicals. 28-days sub-acute toxicity study is carried in female rats at four dose levels (3.12, 31.25, 125 and 500 mg/kg), no changes in observation related death and toxic signs when compared with control. The hematological and biochemical investigation shows a significant change (p>0.05) in the high-level doses (500 mg/kg).
Conclusions: According to the findings of this study, OP extract has the potential to be used to generate new anti-cancer and antioxidant additives for pharmaceutical and food manufacturing. Long-term in vivo toxicological tests should also be conducted to determine a safe dosage of OP extract. |
| first_indexed | 2024-04-12T01:37:51Z |
| format | Article |
| id | doaj.art-e8335a05d5634417861ae08d6b427ec9 |
| institution | Directory Open Access Journal |
| issn | 2372-3416 |
| language | English |
| last_indexed | 2024-04-12T01:37:51Z |
| publishDate | 2022-07-01 |
| publisher | Verduci Editore |
| record_format | Article |
| series | World Cancer Research Journal |
| spelling | doaj.art-e8335a05d5634417861ae08d6b427ec92022-12-22T03:53:17ZengVerduci EditoreWorld Cancer Research Journal2372-34162022-07-01910.32113/wcrj_20227_23592359Assessment of acute and sub-acute toxicity of olive pomace in female Wistar ratsZ. Badi0B. Guermouche1N. Haddam2N. Belyagoubi3K. Rouigueb4H. Benzerjeb5M. Dali-Sahi6Y. Kachekouche7H. Merzouk8Department of Biology, Physiology, Physiopathology and Biochemistry of Nutrition Laboratory, Faculty of Natural and Life Sciences, Earth and Universe, University Abou-Bekr Belkaïd, Tlemcen, AlgeriaDepartment of Biology, Physiology, Physiopathology and Biochemistry of Nutrition Laboratory, Faculty of Natural and Life Sciences, Earth and Universe, University Abou-Bekr Belkaïd, Tlemcen, AlgeriaDepartment of Biology, Physiology, Physiopathology and Biochemistry of Nutrition Laboratory, Faculty of Natural and Life Sciences, Earth and Universe, University Abou-Bekr Belkaïd, Tlemcen, AlgeriaDepartment of Biology, Natural Products Laboratory, Analytical Chemistry and Electrochemistry Laboratory, Abou Bekr Belkaid University, Tlemcen, AlgeriaDepartment of Biology, Physiology, Physiopathology and Biochemistry of Nutrition Laboratory, Faculty of Natural and Life Sciences, Earth and Universe, University Abou-Bekr Belkaïd, Tlemcen, AlgeriaHistopathology Laboratory, Faculty of Medicine, Abou Bekr Belkaid University, Tlemcen, AlgeriaDepartment of Biology, Analytical Chemistry and Electrochemistry Laboratory, Abou Bekr Belkaid University, Tlemcen, AlgeriaDepartment of Biology, Analytical Chemistry and Electrochemistry Laboratory, Abou Bekr Belkaid University, Tlemcen, AlgeriaDepartment of Biology, Physiology, Physiopathology and Biochemistry of Nutrition Laboratory, Faculty of Natural and Life Sciences, Earth and Universe, University Abou-Bekr Belkaïd, Tlemcen, AlgeriaObjective: Olive Pomace (OP) is considered to be a rich source of phenolic compounds. Recently many researches showed a broad biological activity of this by-product of the olive oil production process in addition to their emergence as value-added materials with potential applications in the pharmaceutical, food, and nutraceutical industries. The present study is aimed to evaluate in vivo toxicological activities of OP. Materials and Methods: The qualitative phytochemical analysis aims to determine the key phytoconstituents found in OP. For the in vivo study, two types of tests are performed: acute and 28-day repeated oral toxicity studies in Wistar rats for evaluation of hematological, biochemical, and histological parameters. Results: The qualitative phytochemical analysis has revealed the presence of polyphenols, flavonoids, tannins, saponins, quinones, anthraquinones, terpenoids, and compounds reduced in our methanol extract of OP. In acute oral toxicity, no treatment-related death or toxic signs are observed in female rats for 14 days in 200, 2000, 3000, and 5000 mg/kg doses, besides LD50 value is found to be up to 2000 mg/kg bodyweight. As for the Globally Harmonized System of Classification and Labelling of Chemicals. 28-days sub-acute toxicity study is carried in female rats at four dose levels (3.12, 31.25, 125 and 500 mg/kg), no changes in observation related death and toxic signs when compared with control. The hematological and biochemical investigation shows a significant change (p>0.05) in the high-level doses (500 mg/kg). Conclusions: According to the findings of this study, OP extract has the potential to be used to generate new anti-cancer and antioxidant additives for pharmaceutical and food manufacturing. Long-term in vivo toxicological tests should also be conducted to determine a safe dosage of OP extract.https://www.wcrj.net/wp-content/uploads/sites/5/2022/07/e2359.pdfolive pomacemethanol extractacute toxicitysub-acute toxicity |
| spellingShingle | Z. Badi B. Guermouche N. Haddam N. Belyagoubi K. Rouigueb H. Benzerjeb M. Dali-Sahi Y. Kachekouche H. Merzouk Assessment of acute and sub-acute toxicity of olive pomace in female Wistar rats World Cancer Research Journal olive pomace methanol extract acute toxicity sub-acute toxicity |
| title | Assessment of acute and sub-acute toxicity of olive pomace in female Wistar rats |
| title_full | Assessment of acute and sub-acute toxicity of olive pomace in female Wistar rats |
| title_fullStr | Assessment of acute and sub-acute toxicity of olive pomace in female Wistar rats |
| title_full_unstemmed | Assessment of acute and sub-acute toxicity of olive pomace in female Wistar rats |
| title_short | Assessment of acute and sub-acute toxicity of olive pomace in female Wistar rats |
| title_sort | assessment of acute and sub acute toxicity of olive pomace in female wistar rats |
| topic | olive pomace methanol extract acute toxicity sub-acute toxicity |
| url | https://www.wcrj.net/wp-content/uploads/sites/5/2022/07/e2359.pdf |
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