Changes in soil bacterial and fungal communities in response to Bacillus megaterium NCT-2 inoculation in secondary salinized soil
Background Secondary salinized soil in greenhouses often contains excess nitrate. Inoculation of Bacillus megaterium NCT-2 with nitrate assimilation ability represents an attractive approach for soil remediation. However, the effects of NCT-2 on the structure and function of soil microbial communiti...
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PeerJ Inc.
2021-10-01
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| author | Bin Wang Shaohua Chu Xiaorui Liu Dan Zhang Xiaotong Chai Xijia Yang Yuee Zhi Yaowei Chi Pei Zhou |
| author_facet | Bin Wang Shaohua Chu Xiaorui Liu Dan Zhang Xiaotong Chai Xijia Yang Yuee Zhi Yaowei Chi Pei Zhou |
| author_sort | Bin Wang |
| collection | DOAJ |
| description | Background Secondary salinized soil in greenhouses often contains excess nitrate. Inoculation of Bacillus megaterium NCT-2 with nitrate assimilation ability represents an attractive approach for soil remediation. However, the effects of NCT-2 on the structure and function of soil microbial communities have not been explored. Methods Greenhouse experiments were carried out to investigate changes in soil properties, Brassica chinensis L. growth, bacterial, and fungal community structure and function in response to NCT-2 inoculation. Results The NCT-2 inoculant significantly reduced the nitrate content in B. chinensis and inhibited the rebound of soil nitrate in the later stage. The shifts of bacterial community structure and function by NCT-2 was negligible, and a greater disturbance of soil fungal community structure and function was observed, for example the strong inhibitory effect on ectomycorrhizal fungi. These results indicated that the NCT-2 inoculant likely achieved the remediation effect in secondary salinized soil by shifting fungal community. The present findings add to the current understanding of microbial interactions in response to bacterial inoculation and can be of great significance for the application of NCT-2 inoculants in secondary salinized soil remediation. |
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| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T08:19:03Z |
| publishDate | 2021-10-01 |
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| spelling | doaj.art-e835939b816042c0bb8cc5f42407d6b02023-12-02T21:54:27ZengPeerJ Inc.PeerJ2167-83592021-10-019e1230910.7717/peerj.12309Changes in soil bacterial and fungal communities in response to Bacillus megaterium NCT-2 inoculation in secondary salinized soilBin Wang0Shaohua Chu1Xiaorui Liu2Dan Zhang3Xiaotong Chai4Xijia Yang5Yuee Zhi6Yaowei Chi7Pei Zhou8School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, ChinaBackground Secondary salinized soil in greenhouses often contains excess nitrate. Inoculation of Bacillus megaterium NCT-2 with nitrate assimilation ability represents an attractive approach for soil remediation. However, the effects of NCT-2 on the structure and function of soil microbial communities have not been explored. Methods Greenhouse experiments were carried out to investigate changes in soil properties, Brassica chinensis L. growth, bacterial, and fungal community structure and function in response to NCT-2 inoculation. Results The NCT-2 inoculant significantly reduced the nitrate content in B. chinensis and inhibited the rebound of soil nitrate in the later stage. The shifts of bacterial community structure and function by NCT-2 was negligible, and a greater disturbance of soil fungal community structure and function was observed, for example the strong inhibitory effect on ectomycorrhizal fungi. These results indicated that the NCT-2 inoculant likely achieved the remediation effect in secondary salinized soil by shifting fungal community. The present findings add to the current understanding of microbial interactions in response to bacterial inoculation and can be of great significance for the application of NCT-2 inoculants in secondary salinized soil remediation.https://peerj.com/articles/12309.pdfBacillus megaterium NCT-2NitrateMicrobial communityFUNGuildEctomycorrhizal fungi |
| spellingShingle | Bin Wang Shaohua Chu Xiaorui Liu Dan Zhang Xiaotong Chai Xijia Yang Yuee Zhi Yaowei Chi Pei Zhou Changes in soil bacterial and fungal communities in response to Bacillus megaterium NCT-2 inoculation in secondary salinized soil PeerJ Bacillus megaterium NCT-2 Nitrate Microbial community FUNGuild Ectomycorrhizal fungi |
| title | Changes in soil bacterial and fungal communities in response to Bacillus megaterium NCT-2 inoculation in secondary salinized soil |
| title_full | Changes in soil bacterial and fungal communities in response to Bacillus megaterium NCT-2 inoculation in secondary salinized soil |
| title_fullStr | Changes in soil bacterial and fungal communities in response to Bacillus megaterium NCT-2 inoculation in secondary salinized soil |
| title_full_unstemmed | Changes in soil bacterial and fungal communities in response to Bacillus megaterium NCT-2 inoculation in secondary salinized soil |
| title_short | Changes in soil bacterial and fungal communities in response to Bacillus megaterium NCT-2 inoculation in secondary salinized soil |
| title_sort | changes in soil bacterial and fungal communities in response to bacillus megaterium nct 2 inoculation in secondary salinized soil |
| topic | Bacillus megaterium NCT-2 Nitrate Microbial community FUNGuild Ectomycorrhizal fungi |
| url | https://peerj.com/articles/12309.pdf |
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