Fluorescence in situ hybridization of potato somatohaploids and their somatic hybrid donors using two Solanum brevidens specific sequences
Two Solanum brevidens specific repetitive DNA clones (pSB1 and pSB7) were used simultaneously as probes in fluorescence in situ hybridization (FISH) for cytological studies of somatohaploids and their somatic hybrid donors. pSB1 was labelled with digoxigenin-11-dUTP and pSB7 was labelled with biotin...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Scientific Agricultural Society of Finland
2008-12-01
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| Series: | Agricultural and Food Science |
| Online Access: | https://journal.fi/afs/article/view/5584 |
| _version_ | 1818344810069622784 |
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| author | V. ROKKA N. LAPITAN D. KNUDSON |
| author_facet | V. ROKKA N. LAPITAN D. KNUDSON |
| author_sort | V. ROKKA |
| collection | DOAJ |
| description | Two Solanum brevidens specific repetitive DNA clones (pSB1 and pSB7) were used simultaneously as probes in fluorescence in situ hybridization (FISH) for cytological studies of somatohaploids and their somatic hybrid donors. pSB1 was labelled with digoxigenin-11-dUTP and pSB7 was labelled with biotin-14-dATP and they were detected with reporter molecules conjugated to fluorescent dyes using digital imaging. The tandemly repeated sequences hybridized mostly near the telomeres of the chromosomes of S. brevidens. Using these two probes, it was possible to identify chromosomes containing repetitive DNA of S. brevidens both in the somatic hybrids between S. brevidens and S. tuberosum, and somatohaploids derived from the somatic hybrids. These cytological analyses showed that for the largest part genomes of the hexaploid somatic hybrids and their anther-derived triploid somatohaploids were composed of the genome of S. brevidens.; |
| first_indexed | 2024-12-13T16:52:23Z |
| format | Article |
| id | doaj.art-e838bec5249049bdbb1fa2d16af7fa4a |
| institution | Directory Open Access Journal |
| issn | 1459-6067 1795-1895 |
| language | English |
| last_indexed | 2024-12-13T16:52:23Z |
| publishDate | 2008-12-01 |
| publisher | Scientific Agricultural Society of Finland |
| record_format | Article |
| series | Agricultural and Food Science |
| spelling | doaj.art-e838bec5249049bdbb1fa2d16af7fa4a2022-12-21T23:38:00ZengScientific Agricultural Society of FinlandAgricultural and Food Science1459-60671795-18952008-12-0171Fluorescence in situ hybridization of potato somatohaploids and their somatic hybrid donors using two Solanum brevidens specific sequencesV. ROKKAN. LAPITAND. KNUDSONTwo Solanum brevidens specific repetitive DNA clones (pSB1 and pSB7) were used simultaneously as probes in fluorescence in situ hybridization (FISH) for cytological studies of somatohaploids and their somatic hybrid donors. pSB1 was labelled with digoxigenin-11-dUTP and pSB7 was labelled with biotin-14-dATP and they were detected with reporter molecules conjugated to fluorescent dyes using digital imaging. The tandemly repeated sequences hybridized mostly near the telomeres of the chromosomes of S. brevidens. Using these two probes, it was possible to identify chromosomes containing repetitive DNA of S. brevidens both in the somatic hybrids between S. brevidens and S. tuberosum, and somatohaploids derived from the somatic hybrids. These cytological analyses showed that for the largest part genomes of the hexaploid somatic hybrids and their anther-derived triploid somatohaploids were composed of the genome of S. brevidens.;https://journal.fi/afs/article/view/5584 |
| spellingShingle | V. ROKKA N. LAPITAN D. KNUDSON Fluorescence in situ hybridization of potato somatohaploids and their somatic hybrid donors using two Solanum brevidens specific sequences Agricultural and Food Science |
| title | Fluorescence in situ hybridization of potato somatohaploids and their somatic hybrid donors using two Solanum brevidens specific sequences |
| title_full | Fluorescence in situ hybridization of potato somatohaploids and their somatic hybrid donors using two Solanum brevidens specific sequences |
| title_fullStr | Fluorescence in situ hybridization of potato somatohaploids and their somatic hybrid donors using two Solanum brevidens specific sequences |
| title_full_unstemmed | Fluorescence in situ hybridization of potato somatohaploids and their somatic hybrid donors using two Solanum brevidens specific sequences |
| title_short | Fluorescence in situ hybridization of potato somatohaploids and their somatic hybrid donors using two Solanum brevidens specific sequences |
| title_sort | fluorescence in situ hybridization of potato somatohaploids and their somatic hybrid donors using two solanum brevidens specific sequences |
| url | https://journal.fi/afs/article/view/5584 |
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