Impact of Endoplasmic Reticulum Stress Sensors on Pectolinarin Induced Apoptosis
Pectolinarin, [5,7-Dihydroxy 4′,6-dimethoxyflavone 7-rutinoside, 7-[[6-O-(6-Deoxy-α-L-mannopyranosyl)-β-D-glucopyranosyl] oxy]-5-hydroxy-6-methoxy-2-(4-ethoxyphenyl)-4H-1-benzopyran-4-one], has been stated one of the major compounds in Cirsium nipponicum (Maxim.) Makino. It is characterized by biolo...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2020-09-01
|
| Series: | Frontiers in Public Health |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fpubh.2020.00478/full |
| _version_ | 1818475863613636608 |
|---|---|
| author | Ji-Hye Song Kisang Kwon O-Yu Kwon Eun-Ryeong Lee Seung-Whan Kim Kyung-Hee Kang |
| author_facet | Ji-Hye Song Kisang Kwon O-Yu Kwon Eun-Ryeong Lee Seung-Whan Kim Kyung-Hee Kang |
| author_sort | Ji-Hye Song |
| collection | DOAJ |
| description | Pectolinarin, [5,7-Dihydroxy 4′,6-dimethoxyflavone 7-rutinoside, 7-[[6-O-(6-Deoxy-α-L-mannopyranosyl)-β-D-glucopyranosyl] oxy]-5-hydroxy-6-methoxy-2-(4-ethoxyphenyl)-4H-1-benzopyran-4-one], has been stated one of the major compounds in Cirsium nipponicum (Maxim.) Makino. It is characterized by biological functions of hepatoprotective, anti-inflammatory and antiobesity activities. In this research, it was explained that pectolinarin causes apoptosis in PC12 cells conducted by DNA fragmentation and formation on apoptotic bodies through the activation of ER stress sensors (ATF6 fragmentation and eIF2α phosphorylation). The result of treating the PC12 cells with 50 μM pectolinarin for 24 h has come to increase ATF6 mRNA expression up to 1.6 times, PERK expression up to 1.7 times and IRE1 expression up to 1.4 times, respectively, compared to those of the control. ATF6 fragmentation by pectolinarin treatment was increased about 2 times compared with its control, and phosphorylation of eIF2α was increased 2.5 times. The results proposed that the perception of the molecular mechanisms underlying pectolinarin-caused apoptosis may be useful in new natural medicinal products and health supplements for the apoptosis-related diseases. |
| first_indexed | 2024-12-10T09:18:06Z |
| format | Article |
| id | doaj.art-e83c3d044458493b83ee9ab8a43cc058 |
| institution | Directory Open Access Journal |
| issn | 2296-2565 |
| language | English |
| last_indexed | 2024-12-10T09:18:06Z |
| publishDate | 2020-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Public Health |
| spelling | doaj.art-e83c3d044458493b83ee9ab8a43cc0582022-12-22T01:54:47ZengFrontiers Media S.A.Frontiers in Public Health2296-25652020-09-01810.3389/fpubh.2020.00478571690Impact of Endoplasmic Reticulum Stress Sensors on Pectolinarin Induced ApoptosisJi-Hye Song0Kisang Kwon1O-Yu Kwon2Eun-Ryeong Lee3Seung-Whan Kim4Kyung-Hee Kang5Departments of Anatomy and Cell Biology, College of Medicine, Chungnam National University, Daejeon, South KoreaDepartments of Anatomy and Cell Biology, College of Medicine, Chungnam National University, Daejeon, South KoreaDepartments of Anatomy and Cell Biology, College of Medicine, Chungnam National University, Daejeon, South KoreaDepartment of Biomedical Laboratory Science, College of Health and Welfare, Kyungwoon University, Gumi, South KoreaDepartment of Emergency Medicine, College of Medicine, Chungnam National University, Daejeon, South KoreaDepartment of Dental Hygiene, College of Medical Science, Konyang University, Daejeon, South KoreaPectolinarin, [5,7-Dihydroxy 4′,6-dimethoxyflavone 7-rutinoside, 7-[[6-O-(6-Deoxy-α-L-mannopyranosyl)-β-D-glucopyranosyl] oxy]-5-hydroxy-6-methoxy-2-(4-ethoxyphenyl)-4H-1-benzopyran-4-one], has been stated one of the major compounds in Cirsium nipponicum (Maxim.) Makino. It is characterized by biological functions of hepatoprotective, anti-inflammatory and antiobesity activities. In this research, it was explained that pectolinarin causes apoptosis in PC12 cells conducted by DNA fragmentation and formation on apoptotic bodies through the activation of ER stress sensors (ATF6 fragmentation and eIF2α phosphorylation). The result of treating the PC12 cells with 50 μM pectolinarin for 24 h has come to increase ATF6 mRNA expression up to 1.6 times, PERK expression up to 1.7 times and IRE1 expression up to 1.4 times, respectively, compared to those of the control. ATF6 fragmentation by pectolinarin treatment was increased about 2 times compared with its control, and phosphorylation of eIF2α was increased 2.5 times. The results proposed that the perception of the molecular mechanisms underlying pectolinarin-caused apoptosis may be useful in new natural medicinal products and health supplements for the apoptosis-related diseases.https://www.frontiersin.org/article/10.3389/fpubh.2020.00478/fullpectolinarinPC12 cellsendoplasmic reticulum (ER)stresssensor |
| spellingShingle | Ji-Hye Song Kisang Kwon O-Yu Kwon Eun-Ryeong Lee Seung-Whan Kim Kyung-Hee Kang Impact of Endoplasmic Reticulum Stress Sensors on Pectolinarin Induced Apoptosis Frontiers in Public Health pectolinarin PC12 cells endoplasmic reticulum (ER) stress sensor |
| title | Impact of Endoplasmic Reticulum Stress Sensors on Pectolinarin Induced Apoptosis |
| title_full | Impact of Endoplasmic Reticulum Stress Sensors on Pectolinarin Induced Apoptosis |
| title_fullStr | Impact of Endoplasmic Reticulum Stress Sensors on Pectolinarin Induced Apoptosis |
| title_full_unstemmed | Impact of Endoplasmic Reticulum Stress Sensors on Pectolinarin Induced Apoptosis |
| title_short | Impact of Endoplasmic Reticulum Stress Sensors on Pectolinarin Induced Apoptosis |
| title_sort | impact of endoplasmic reticulum stress sensors on pectolinarin induced apoptosis |
| topic | pectolinarin PC12 cells endoplasmic reticulum (ER) stress sensor |
| url | https://www.frontiersin.org/article/10.3389/fpubh.2020.00478/full |
| work_keys_str_mv | AT jihyesong impactofendoplasmicreticulumstresssensorsonpectolinarininducedapoptosis AT kisangkwon impactofendoplasmicreticulumstresssensorsonpectolinarininducedapoptosis AT oyukwon impactofendoplasmicreticulumstresssensorsonpectolinarininducedapoptosis AT eunryeonglee impactofendoplasmicreticulumstresssensorsonpectolinarininducedapoptosis AT seungwhankim impactofendoplasmicreticulumstresssensorsonpectolinarininducedapoptosis AT kyungheekang impactofendoplasmicreticulumstresssensorsonpectolinarininducedapoptosis |