Deep Immunophenotyping of Circulating T and B Cells in Relapsing Adult-Onset Still’s Disease
Adult-onset Still’s disease (AOSD) is a complex systemic inflammatory disorder, categorized as an ‘IL-1 driven’ inflammasomapathy. Despite this, the interaction between T and B cells remains poorly understood. We conducted a study, enrolling 7 patients with relapsing AOSD and 15 healthy control subj...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-02-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/46/2/75 |
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| author | Valentina Myachikova Igor Kudryavtsev Artem Rubinstein Arthur Aquino Dmitry Isakov Alexey Golovkin Alexey Maslyanskiy |
| author_facet | Valentina Myachikova Igor Kudryavtsev Artem Rubinstein Arthur Aquino Dmitry Isakov Alexey Golovkin Alexey Maslyanskiy |
| author_sort | Valentina Myachikova |
| collection | DOAJ |
| description | Adult-onset Still’s disease (AOSD) is a complex systemic inflammatory disorder, categorized as an ‘IL-1 driven’ inflammasomapathy. Despite this, the interaction between T and B cells remains poorly understood. We conducted a study, enrolling 7 patients with relapsing AOSD and 15 healthy control subjects, utilizing deep flow cytometry analysis to examine peripheral blood T- and B-cell subsets. T-cell and B-cell subsets were significantly altered in patients with AOSD. Within CD4+ T cells, Th2 cells were decreased. Additionally, Th17 cell and follicular Th cell subsets were altered within CD45RA–CD62L+ and CD45RA–CD62L– Th cells in patients with AOSD compared to healthy controls. We identified changes in CD8+ T cell maturation and ‘polarization’ in AOSD patients, with an elevated presence of the TEMRA CD8+ T cell subset. Furthermore, the percentage of Tc1 cells was decreased, while the frequency of CCR6–CXCR3– Tc2 cells was elevated. Finally, we determined that the frequency of CD5+CD27– B cells was dramatically decreased in patients with AOSD compared to healthy controls. Further investigations on a large group of patients with AOSD are required to evaluate these adaptive immunity cells in the disease pathogenesis. |
| first_indexed | 2024-03-07T22:38:11Z |
| format | Article |
| id | doaj.art-e83c9a1ddf364f8e9745ee05db004c94 |
| institution | Directory Open Access Journal |
| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-07T22:38:11Z |
| publishDate | 2024-02-01 |
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| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj.art-e83c9a1ddf364f8e9745ee05db004c942024-02-23T15:12:35ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452024-02-014621177119110.3390/cimb46020075Deep Immunophenotyping of Circulating T and B Cells in Relapsing Adult-Onset Still’s DiseaseValentina Myachikova0Igor Kudryavtsev1Artem Rubinstein2Arthur Aquino3Dmitry Isakov4Alexey Golovkin5Alexey Maslyanskiy6Rheumatology and Immunopathology Research Laboratory, Federal State Budgetary Institution “Almazov National Medical Research Centre” of the Ministry of Health of the Russian Federation, 197341 St. Petersburg, RussiaAutoimmune and Autoinflammatory Diseases Research Laboratory, Federal State Budgetary Institution “Almazov National Medical Research Centre” of the Ministry of Health of the Russian Federation, 197341 St. Petersburg, RussiaAutoimmune and Autoinflammatory Diseases Research Laboratory, Federal State Budgetary Institution “Almazov National Medical Research Centre” of the Ministry of Health of the Russian Federation, 197341 St. Petersburg, RussiaAutoimmune and Autoinflammatory Diseases Research Laboratory, Federal State Budgetary Institution “Almazov National Medical Research Centre” of the Ministry of Health of the Russian Federation, 197341 St. Petersburg, RussiaDepartment of Immunology, First St. Petersburg State Medical University, 197022 St. Petersburg, RussiaAutoimmune and Autoinflammatory Diseases Research Laboratory, Federal State Budgetary Institution “Almazov National Medical Research Centre” of the Ministry of Health of the Russian Federation, 197341 St. Petersburg, RussiaRheumatology and Immunopathology Research Laboratory, Federal State Budgetary Institution “Almazov National Medical Research Centre” of the Ministry of Health of the Russian Federation, 197341 St. Petersburg, RussiaAdult-onset Still’s disease (AOSD) is a complex systemic inflammatory disorder, categorized as an ‘IL-1 driven’ inflammasomapathy. Despite this, the interaction between T and B cells remains poorly understood. We conducted a study, enrolling 7 patients with relapsing AOSD and 15 healthy control subjects, utilizing deep flow cytometry analysis to examine peripheral blood T- and B-cell subsets. T-cell and B-cell subsets were significantly altered in patients with AOSD. Within CD4+ T cells, Th2 cells were decreased. Additionally, Th17 cell and follicular Th cell subsets were altered within CD45RA–CD62L+ and CD45RA–CD62L– Th cells in patients with AOSD compared to healthy controls. We identified changes in CD8+ T cell maturation and ‘polarization’ in AOSD patients, with an elevated presence of the TEMRA CD8+ T cell subset. Furthermore, the percentage of Tc1 cells was decreased, while the frequency of CCR6–CXCR3– Tc2 cells was elevated. Finally, we determined that the frequency of CD5+CD27– B cells was dramatically decreased in patients with AOSD compared to healthy controls. Further investigations on a large group of patients with AOSD are required to evaluate these adaptive immunity cells in the disease pathogenesis.https://www.mdpi.com/1467-3045/46/2/75adult-onset Still’s diseaseAOSDautoinflammation‘IL-1 driven’ diseaseTh cellsCD8+ T cells |
| spellingShingle | Valentina Myachikova Igor Kudryavtsev Artem Rubinstein Arthur Aquino Dmitry Isakov Alexey Golovkin Alexey Maslyanskiy Deep Immunophenotyping of Circulating T and B Cells in Relapsing Adult-Onset Still’s Disease Current Issues in Molecular Biology adult-onset Still’s disease AOSD autoinflammation ‘IL-1 driven’ disease Th cells CD8+ T cells |
| title | Deep Immunophenotyping of Circulating T and B Cells in Relapsing Adult-Onset Still’s Disease |
| title_full | Deep Immunophenotyping of Circulating T and B Cells in Relapsing Adult-Onset Still’s Disease |
| title_fullStr | Deep Immunophenotyping of Circulating T and B Cells in Relapsing Adult-Onset Still’s Disease |
| title_full_unstemmed | Deep Immunophenotyping of Circulating T and B Cells in Relapsing Adult-Onset Still’s Disease |
| title_short | Deep Immunophenotyping of Circulating T and B Cells in Relapsing Adult-Onset Still’s Disease |
| title_sort | deep immunophenotyping of circulating t and b cells in relapsing adult onset still s disease |
| topic | adult-onset Still’s disease AOSD autoinflammation ‘IL-1 driven’ disease Th cells CD8+ T cells |
| url | https://www.mdpi.com/1467-3045/46/2/75 |
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