Comprehensive relative quantitative metabolomics analysis of lycopodium alkaloids in different tissues of Huperzia serrata
Qian ceng Ta, the whole plant of Huperzia serrata, is an important landscape and medicinal herbs and contains abundant bioactive lycopodium alkaloids. Although the structures of more than 100 lycopodium alkaloids in Huperzia serrata have been isolated and identified, the content and distribution of...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
KeAi Communications Co., Ltd.
2018-03-01
|
| Series: | Synthetic and Systems Biotechnology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405805X17301072 |
| _version_ | 1797203949140312064 |
|---|---|
| author | Shiwen Wu Zhen Fan Youli Xiao |
| author_facet | Shiwen Wu Zhen Fan Youli Xiao |
| author_sort | Shiwen Wu |
| collection | DOAJ |
| description | Qian ceng Ta, the whole plant of Huperzia serrata, is an important landscape and medicinal herbs and contains abundant bioactive lycopodium alkaloids. Although the structures of more than 100 lycopodium alkaloids in Huperzia serrata have been isolated and identified, the content and distribution of these alkaloids in different tissues are still unclear. In current study, an ultra-performance liquid chromatography-mass spectrometry based comprehensive metabolomics strategy was developed, including the extraction, separation, identification, and statistical analysis. The results showed that different types lycopodium alkaloids could be separated at different time-windows, which was helpful for further metabolite identification. Peak4388 and peak3954 were metabolite biomarkers for the different tissues according to the principle component analysis and partial least squares-discriminant analysis model. A computational tool based in-house database was also built up and used for putative identification. Of the 2354 true peaks after four-step filtration, 118 peaks were putatively identified as lycopodium alkaloids by using in-house database, and four of which was identified by authentic standards. Alternatively, another computational software was used to predict the fragmentation pattern, to dereplicate the structure of identified peaks, and identified the peak3585 to N-methylhuperzine A. The integration of both computational tools could be used for more metabolites identification. |
| first_indexed | 2024-04-24T08:27:27Z |
| format | Article |
| id | doaj.art-e841b817808c420786e68933d4ec7531 |
| institution | Directory Open Access Journal |
| issn | 2405-805X |
| language | English |
| last_indexed | 2024-04-24T08:27:27Z |
| publishDate | 2018-03-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Synthetic and Systems Biotechnology |
| spelling | doaj.art-e841b817808c420786e68933d4ec75312024-04-16T21:41:06ZengKeAi Communications Co., Ltd.Synthetic and Systems Biotechnology2405-805X2018-03-0131445510.1016/j.synbio.2017.12.003Comprehensive relative quantitative metabolomics analysis of lycopodium alkaloids in different tissues of Huperzia serrataShiwen Wu0Zhen Fan1Youli Xiao2CAS Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, ChinaCAS Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, ChinaCAS Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, ChinaQian ceng Ta, the whole plant of Huperzia serrata, is an important landscape and medicinal herbs and contains abundant bioactive lycopodium alkaloids. Although the structures of more than 100 lycopodium alkaloids in Huperzia serrata have been isolated and identified, the content and distribution of these alkaloids in different tissues are still unclear. In current study, an ultra-performance liquid chromatography-mass spectrometry based comprehensive metabolomics strategy was developed, including the extraction, separation, identification, and statistical analysis. The results showed that different types lycopodium alkaloids could be separated at different time-windows, which was helpful for further metabolite identification. Peak4388 and peak3954 were metabolite biomarkers for the different tissues according to the principle component analysis and partial least squares-discriminant analysis model. A computational tool based in-house database was also built up and used for putative identification. Of the 2354 true peaks after four-step filtration, 118 peaks were putatively identified as lycopodium alkaloids by using in-house database, and four of which was identified by authentic standards. Alternatively, another computational software was used to predict the fragmentation pattern, to dereplicate the structure of identified peaks, and identified the peak3585 to N-methylhuperzine A. The integration of both computational tools could be used for more metabolites identification.http://www.sciencedirect.com/science/article/pii/S2405805X17301072Huperzia serrataDifferent tissuesMetabolomicsMetabolite identificationIn-silico fragmentations prediction |
| spellingShingle | Shiwen Wu Zhen Fan Youli Xiao Comprehensive relative quantitative metabolomics analysis of lycopodium alkaloids in different tissues of Huperzia serrata Synthetic and Systems Biotechnology Huperzia serrata Different tissues Metabolomics Metabolite identification In-silico fragmentations prediction |
| title | Comprehensive relative quantitative metabolomics analysis of lycopodium alkaloids in different tissues of Huperzia serrata |
| title_full | Comprehensive relative quantitative metabolomics analysis of lycopodium alkaloids in different tissues of Huperzia serrata |
| title_fullStr | Comprehensive relative quantitative metabolomics analysis of lycopodium alkaloids in different tissues of Huperzia serrata |
| title_full_unstemmed | Comprehensive relative quantitative metabolomics analysis of lycopodium alkaloids in different tissues of Huperzia serrata |
| title_short | Comprehensive relative quantitative metabolomics analysis of lycopodium alkaloids in different tissues of Huperzia serrata |
| title_sort | comprehensive relative quantitative metabolomics analysis of lycopodium alkaloids in different tissues of huperzia serrata |
| topic | Huperzia serrata Different tissues Metabolomics Metabolite identification In-silico fragmentations prediction |
| url | http://www.sciencedirect.com/science/article/pii/S2405805X17301072 |
| work_keys_str_mv | AT shiwenwu comprehensiverelativequantitativemetabolomicsanalysisoflycopodiumalkaloidsindifferenttissuesofhuperziaserrata AT zhenfan comprehensiverelativequantitativemetabolomicsanalysisoflycopodiumalkaloidsindifferenttissuesofhuperziaserrata AT youlixiao comprehensiverelativequantitativemetabolomicsanalysisoflycopodiumalkaloidsindifferenttissuesofhuperziaserrata |