Estimation of Ideal Visible Spectrum Wavelength for Optimal ABO Blood Grouping using Spectrophotometry
Introduction: The most widely accepted and used method of blood typing is the agglutination test with the antibodies A and B for identifying ABO blood group and a separate antibody D is used for giving Rh (Rhesus) type of the sample under study. The problem faced by this method is that it consum...
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JCDR Research and Publications Pvt. Ltd.
2019-01-01
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Series: | National Journal of Laboratory Medicine |
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Online Access: | http://www.njlm.net/articles/PDF/2339/37542_CE[Ra1]_F(AC)_PF1(A_SHU)_PFA(SHU)_PN(SHU).pdf |
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author | L Suganthi S Viswanath B Sangeetha S Om Prakash |
author_facet | L Suganthi S Viswanath B Sangeetha S Om Prakash |
author_sort | L Suganthi |
collection | DOAJ |
description | Introduction: The most widely accepted and used method of
blood typing is the agglutination test with the antibodies A and
B for identifying ABO blood group and a separate antibody D is
used for giving Rh (Rhesus) type of the sample under study. The
problem faced by this method is that it consumes more time
and technician’s alertness in case of large number of samples.
Thus, optical ABO blood grouping system with appropriate
wavelength will improve the blood grouping with better accuracy
and time efficiency.
Aim: To determine the ideal wavelength of visible spectrum
which has maximum absorbance with the agglutinated blood
sample using spectrophotometry so that the estimated
wavelength can be implemented as light source in an optical
blood typing system for better results.
Materials and Methods: An observational study has been
performed on nine subjects which was conducted at Sri
Sivasubramaniya Nadar College of Engineering (SSNCE) Tamil
Nadu, India. The subject’s blood were collected and mixed with
saline. Antibodies A, B, D are added and the data of specific
blood absorbance values were measured using Spectrometry.
Obtained data were tabulated and graphically represented
using Microsoft Excel.
Results: The highest absorbance of sample corresponds to the
wavelength in the range of 425-475 nm. Thus, this wavelength
range is most suitable and ideal visible spectrum range
for optimal ABO blood grouping using spectrophotometer.
Significant difference of absorbance between various types of
positive and negative samples is obtained.
Conclusion: A difference in absorbance of 0.3 is seen between
negative and positive samples. With further research and
study, it is possible to separate the specific blood group after
identification of the positive and negative types. |
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institution | Directory Open Access Journal |
issn | 2277-8551 2455-6882 |
language | English |
last_indexed | 2024-04-13T15:52:29Z |
publishDate | 2019-01-01 |
publisher | JCDR Research and Publications Pvt. Ltd. |
record_format | Article |
series | National Journal of Laboratory Medicine |
spelling | doaj.art-e8440e0a9ea3428c8a4349b2e8a8ec6b2022-12-22T02:40:49ZengJCDR Research and Publications Pvt. Ltd.National Journal of Laboratory Medicine2277-85512455-68822019-01-0181BE01BE0510.7860/NJLM/2019/37542:2339Estimation of Ideal Visible Spectrum Wavelength for Optimal ABO Blood Grouping using SpectrophotometryL Suganthi0S Viswanath1B Sangeetha2S Om Prakash3Associate Professor, Department of Biomedical, Sri Sivasubramaniya Nadar College of Engineering, Chennai, Tamil Nadu, IndiaStudent, Department of Biomedical, Sri Sivasubramaniya Nadar College of Engineering, Chennai, Tamil Nadu, India.Student, Department of Biomedical, Sri Sivasubramaniya Nadar College of Engineering, Chennai, Tamil Nadu, India.Student, Department of Biomedical, Sri Sivasubramaniya Nadar College of Engineering, Chennai, Tamil Nadu, India.Introduction: The most widely accepted and used method of blood typing is the agglutination test with the antibodies A and B for identifying ABO blood group and a separate antibody D is used for giving Rh (Rhesus) type of the sample under study. The problem faced by this method is that it consumes more time and technician’s alertness in case of large number of samples. Thus, optical ABO blood grouping system with appropriate wavelength will improve the blood grouping with better accuracy and time efficiency. Aim: To determine the ideal wavelength of visible spectrum which has maximum absorbance with the agglutinated blood sample using spectrophotometry so that the estimated wavelength can be implemented as light source in an optical blood typing system for better results. Materials and Methods: An observational study has been performed on nine subjects which was conducted at Sri Sivasubramaniya Nadar College of Engineering (SSNCE) Tamil Nadu, India. The subject’s blood were collected and mixed with saline. Antibodies A, B, D are added and the data of specific blood absorbance values were measured using Spectrometry. Obtained data were tabulated and graphically represented using Microsoft Excel. Results: The highest absorbance of sample corresponds to the wavelength in the range of 425-475 nm. Thus, this wavelength range is most suitable and ideal visible spectrum range for optimal ABO blood grouping using spectrophotometer. Significant difference of absorbance between various types of positive and negative samples is obtained. Conclusion: A difference in absorbance of 0.3 is seen between negative and positive samples. With further research and study, it is possible to separate the specific blood group after identification of the positive and negative types.http://www.njlm.net/articles/PDF/2339/37542_CE[Ra1]_F(AC)_PF1(A_SHU)_PFA(SHU)_PN(SHU).pdfabsorbanceagglutination testantibodiesblood group |
spellingShingle | L Suganthi S Viswanath B Sangeetha S Om Prakash Estimation of Ideal Visible Spectrum Wavelength for Optimal ABO Blood Grouping using Spectrophotometry National Journal of Laboratory Medicine absorbance agglutination test antibodies blood group |
title | Estimation of Ideal Visible Spectrum Wavelength for Optimal ABO Blood Grouping using Spectrophotometry |
title_full | Estimation of Ideal Visible Spectrum Wavelength for Optimal ABO Blood Grouping using Spectrophotometry |
title_fullStr | Estimation of Ideal Visible Spectrum Wavelength for Optimal ABO Blood Grouping using Spectrophotometry |
title_full_unstemmed | Estimation of Ideal Visible Spectrum Wavelength for Optimal ABO Blood Grouping using Spectrophotometry |
title_short | Estimation of Ideal Visible Spectrum Wavelength for Optimal ABO Blood Grouping using Spectrophotometry |
title_sort | estimation of ideal visible spectrum wavelength for optimal abo blood grouping using spectrophotometry |
topic | absorbance agglutination test antibodies blood group |
url | http://www.njlm.net/articles/PDF/2339/37542_CE[Ra1]_F(AC)_PF1(A_SHU)_PFA(SHU)_PN(SHU).pdf |
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