Erectile Dysfunction Treatment Using Stem Cell Delivery Patch in a Cavernous Nerve Injury Rat Model
Erectile dysfunction (ED) is a common and feared complication of radical prostatectomy (RP) for prostate cancer. Recently, tissue engineering for post-prostatectomy ED has been attempted in which controlled interactions between cells, growth factors, and the extracellular matrix (ECM) are important...
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| Format: | Article |
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MDPI AG
2023-05-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/10/6/635 |
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| author | Hyong Woo Moon In Gul Kim Mee Young Kim Ae Ryang Jung Kwideok Park Ji Youl Lee |
| author_facet | Hyong Woo Moon In Gul Kim Mee Young Kim Ae Ryang Jung Kwideok Park Ji Youl Lee |
| author_sort | Hyong Woo Moon |
| collection | DOAJ |
| description | Erectile dysfunction (ED) is a common and feared complication of radical prostatectomy (RP) for prostate cancer. Recently, tissue engineering for post-prostatectomy ED has been attempted in which controlled interactions between cells, growth factors, and the extracellular matrix (ECM) are important for the structural integrity if nerve regeneration. In this study, we evaluated the effects of a biomechanical ECM patch on the morphology and behavior of human bone marrow-derived mesenchymal stem cells (hBMSCs) in a bilateral cavernous nerve injury (BCNI) rat model. The ECM patch, made of decellularized human fibroblast-derived ECM (hFDM) and a biocompatible polyvinyl alcohol (PVA) hydrogel, was tested with human bone marrow-derived mesenchymal stem cells (hBMSCs) on a bilateral cavernous nerve injury (BCNI) rat model. In vitro analysis showed that the hFDM/PVA + hBMSCs patches significantly increased neural development markers. In vivo experiments demonstrated that the rats treated with the hFDM/PVA patch had higher ICP/MAP ratios, higher ratios of smooth muscle to collagen, increased nNOS content, higher levels of eNOS protein expression, and higher cGMP levels compared to the BCNI group. These results indicate that the hFDM/PVA patch is effective in promoting angiogenesis, smooth muscle regeneration, and nitrergic nerve regeneration, which could contribute to improved erectile function in post-prostatectomy ED. |
| first_indexed | 2024-03-11T02:46:17Z |
| format | Article |
| id | doaj.art-e844fc956f404c40948deb4a9f185c3a |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-11T02:46:17Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-e844fc956f404c40948deb4a9f185c3a2023-11-18T09:20:40ZengMDPI AGBioengineering2306-53542023-05-0110663510.3390/bioengineering10060635Erectile Dysfunction Treatment Using Stem Cell Delivery Patch in a Cavernous Nerve Injury Rat ModelHyong Woo Moon0In Gul Kim1Mee Young Kim2Ae Ryang Jung3Kwideok Park4Ji Youl Lee5Department of Urology, Seoul St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of KoreaDepartment of Otorhinolaryngology-Head and Neck Surgery, Seoul National University Hospital, Seoul 03080, Republic of KoreaCatholic Cancer Research Institute, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of KoreaDepartment of Urology, Seoul St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of KoreaCenter for Biomaterials, Korea Institute of Science and Technology (KIST), Seoul 02792, Republic of KoreaDepartment of Urology, Seoul St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of KoreaErectile dysfunction (ED) is a common and feared complication of radical prostatectomy (RP) for prostate cancer. Recently, tissue engineering for post-prostatectomy ED has been attempted in which controlled interactions between cells, growth factors, and the extracellular matrix (ECM) are important for the structural integrity if nerve regeneration. In this study, we evaluated the effects of a biomechanical ECM patch on the morphology and behavior of human bone marrow-derived mesenchymal stem cells (hBMSCs) in a bilateral cavernous nerve injury (BCNI) rat model. The ECM patch, made of decellularized human fibroblast-derived ECM (hFDM) and a biocompatible polyvinyl alcohol (PVA) hydrogel, was tested with human bone marrow-derived mesenchymal stem cells (hBMSCs) on a bilateral cavernous nerve injury (BCNI) rat model. In vitro analysis showed that the hFDM/PVA + hBMSCs patches significantly increased neural development markers. In vivo experiments demonstrated that the rats treated with the hFDM/PVA patch had higher ICP/MAP ratios, higher ratios of smooth muscle to collagen, increased nNOS content, higher levels of eNOS protein expression, and higher cGMP levels compared to the BCNI group. These results indicate that the hFDM/PVA patch is effective in promoting angiogenesis, smooth muscle regeneration, and nitrergic nerve regeneration, which could contribute to improved erectile function in post-prostatectomy ED.https://www.mdpi.com/2306-5354/10/6/635rectile dysfunctionstem cell deliveryprostate cancerbiofabrication |
| spellingShingle | Hyong Woo Moon In Gul Kim Mee Young Kim Ae Ryang Jung Kwideok Park Ji Youl Lee Erectile Dysfunction Treatment Using Stem Cell Delivery Patch in a Cavernous Nerve Injury Rat Model Bioengineering rectile dysfunction stem cell delivery prostate cancer biofabrication |
| title | Erectile Dysfunction Treatment Using Stem Cell Delivery Patch in a Cavernous Nerve Injury Rat Model |
| title_full | Erectile Dysfunction Treatment Using Stem Cell Delivery Patch in a Cavernous Nerve Injury Rat Model |
| title_fullStr | Erectile Dysfunction Treatment Using Stem Cell Delivery Patch in a Cavernous Nerve Injury Rat Model |
| title_full_unstemmed | Erectile Dysfunction Treatment Using Stem Cell Delivery Patch in a Cavernous Nerve Injury Rat Model |
| title_short | Erectile Dysfunction Treatment Using Stem Cell Delivery Patch in a Cavernous Nerve Injury Rat Model |
| title_sort | erectile dysfunction treatment using stem cell delivery patch in a cavernous nerve injury rat model |
| topic | rectile dysfunction stem cell delivery prostate cancer biofabrication |
| url | https://www.mdpi.com/2306-5354/10/6/635 |
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