An evidence based hypothesis on the existence of two pathways of mitochondrial crista formation
Metabolic function and architecture of mitochondria are intimately linked. More than 60 years ago, cristae were discovered as characteristic elements of mitochondria that harbor the protein complexes of oxidative phosphorylation, but how cristae are formed, remained an open question. Here we present...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2016-11-01
|
| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/18853 |
| _version_ | 1828375309386252288 |
|---|---|
| author | Max E Harner Ann-Katrin Unger Willie JC Geerts Muriel Mari Toshiaki Izawa Maria Stenger Stefan Geimer Fulvio Reggiori Benedikt Westermann Walter Neupert |
| author_facet | Max E Harner Ann-Katrin Unger Willie JC Geerts Muriel Mari Toshiaki Izawa Maria Stenger Stefan Geimer Fulvio Reggiori Benedikt Westermann Walter Neupert |
| author_sort | Max E Harner |
| collection | DOAJ |
| description | Metabolic function and architecture of mitochondria are intimately linked. More than 60 years ago, cristae were discovered as characteristic elements of mitochondria that harbor the protein complexes of oxidative phosphorylation, but how cristae are formed, remained an open question. Here we present experimental results obtained with yeast that support a novel hypothesis on the existence of two molecular pathways that lead to the generation of lamellar and tubular cristae. Formation of lamellar cristae depends on the mitochondrial fusion machinery through a pathway that is required also for homeostasis of mitochondria and mitochondrial DNA. Tubular cristae are formed via invaginations of the inner boundary membrane by a pathway independent of the fusion machinery. Dimerization of the F1FO-ATP synthase and the presence of the MICOS complex are necessary for both pathways. The proposed hypothesis is suggested to apply also to higher eukaryotes, since the key components are conserved in structure and function throughout evolution. |
| first_indexed | 2024-04-14T07:45:37Z |
| format | Article |
| id | doaj.art-e8461339cad64b2fb5a7ee4a41d66d0f |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-14T07:45:37Z |
| publishDate | 2016-11-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-e8461339cad64b2fb5a7ee4a41d66d0f2022-12-22T02:05:22ZengeLife Sciences Publications LtdeLife2050-084X2016-11-01510.7554/eLife.18853An evidence based hypothesis on the existence of two pathways of mitochondrial crista formationMax E Harner0Ann-Katrin Unger1Willie JC Geerts2Muriel Mari3Toshiaki Izawa4Maria Stenger5Stefan Geimer6Fulvio Reggiori7Benedikt Westermann8https://orcid.org/0000-0002-2991-1604Walter Neupert9https://orcid.org/0000-0003-0571-4419Max Planck Institute of Biochemistry, Martinsried, Germany; Walter Brendel Centre of Experimental Medicine, Ludwig-Maximilians-Universität München, Martinsried, GermanyCell Biology and Electron Microscopy, Universität Bayreuth, Bayreuth, GermanyBiomolecular Imaging, Bijvoet Center, Universiteit Utrecht, Utrecht, NetherlandsDepartment of Cell Biology, University Medical Center Groningen, University of Groningen, Groningen, NetherlandsMax Planck Institute of Biochemistry, Martinsried, GermanyCell Biology and Electron Microscopy, Universität Bayreuth, Bayreuth, GermanyCell Biology and Electron Microscopy, Universität Bayreuth, Bayreuth, GermanyDepartment of Cell Biology, University Medical Center Groningen, University of Groningen, Groningen, NetherlandsCell Biology and Electron Microscopy, Universität Bayreuth, Bayreuth, GermanyMax Planck Institute of Biochemistry, Martinsried, Germany; Department of Anatomy and Cell Biology, Biomedical Center, Ludwig-Maximilians Universität München, Martinsried, GermanyMetabolic function and architecture of mitochondria are intimately linked. More than 60 years ago, cristae were discovered as characteristic elements of mitochondria that harbor the protein complexes of oxidative phosphorylation, but how cristae are formed, remained an open question. Here we present experimental results obtained with yeast that support a novel hypothesis on the existence of two molecular pathways that lead to the generation of lamellar and tubular cristae. Formation of lamellar cristae depends on the mitochondrial fusion machinery through a pathway that is required also for homeostasis of mitochondria and mitochondrial DNA. Tubular cristae are formed via invaginations of the inner boundary membrane by a pathway independent of the fusion machinery. Dimerization of the F1FO-ATP synthase and the presence of the MICOS complex are necessary for both pathways. The proposed hypothesis is suggested to apply also to higher eukaryotes, since the key components are conserved in structure and function throughout evolution.https://elifesciences.org/articles/18853mitochondriacrista formationmitochondrial fusionMgm1/Opa1MICOSF1FO-ATP synthase |
| spellingShingle | Max E Harner Ann-Katrin Unger Willie JC Geerts Muriel Mari Toshiaki Izawa Maria Stenger Stefan Geimer Fulvio Reggiori Benedikt Westermann Walter Neupert An evidence based hypothesis on the existence of two pathways of mitochondrial crista formation eLife mitochondria crista formation mitochondrial fusion Mgm1/Opa1 MICOS F1FO-ATP synthase |
| title | An evidence based hypothesis on the existence of two pathways of mitochondrial crista formation |
| title_full | An evidence based hypothesis on the existence of two pathways of mitochondrial crista formation |
| title_fullStr | An evidence based hypothesis on the existence of two pathways of mitochondrial crista formation |
| title_full_unstemmed | An evidence based hypothesis on the existence of two pathways of mitochondrial crista formation |
| title_short | An evidence based hypothesis on the existence of two pathways of mitochondrial crista formation |
| title_sort | evidence based hypothesis on the existence of two pathways of mitochondrial crista formation |
| topic | mitochondria crista formation mitochondrial fusion Mgm1/Opa1 MICOS F1FO-ATP synthase |
| url | https://elifesciences.org/articles/18853 |
| work_keys_str_mv | AT maxeharner anevidencebasedhypothesisontheexistenceoftwopathwaysofmitochondrialcristaformation AT annkatrinunger anevidencebasedhypothesisontheexistenceoftwopathwaysofmitochondrialcristaformation AT williejcgeerts anevidencebasedhypothesisontheexistenceoftwopathwaysofmitochondrialcristaformation AT murielmari anevidencebasedhypothesisontheexistenceoftwopathwaysofmitochondrialcristaformation AT toshiakiizawa anevidencebasedhypothesisontheexistenceoftwopathwaysofmitochondrialcristaformation AT mariastenger anevidencebasedhypothesisontheexistenceoftwopathwaysofmitochondrialcristaformation AT stefangeimer anevidencebasedhypothesisontheexistenceoftwopathwaysofmitochondrialcristaformation AT fulvioreggiori anevidencebasedhypothesisontheexistenceoftwopathwaysofmitochondrialcristaformation AT benediktwestermann anevidencebasedhypothesisontheexistenceoftwopathwaysofmitochondrialcristaformation AT walterneupert anevidencebasedhypothesisontheexistenceoftwopathwaysofmitochondrialcristaformation AT maxeharner evidencebasedhypothesisontheexistenceoftwopathwaysofmitochondrialcristaformation AT annkatrinunger evidencebasedhypothesisontheexistenceoftwopathwaysofmitochondrialcristaformation AT williejcgeerts evidencebasedhypothesisontheexistenceoftwopathwaysofmitochondrialcristaformation AT murielmari evidencebasedhypothesisontheexistenceoftwopathwaysofmitochondrialcristaformation AT toshiakiizawa evidencebasedhypothesisontheexistenceoftwopathwaysofmitochondrialcristaformation AT mariastenger evidencebasedhypothesisontheexistenceoftwopathwaysofmitochondrialcristaformation AT stefangeimer evidencebasedhypothesisontheexistenceoftwopathwaysofmitochondrialcristaformation AT fulvioreggiori evidencebasedhypothesisontheexistenceoftwopathwaysofmitochondrialcristaformation AT benediktwestermann evidencebasedhypothesisontheexistenceoftwopathwaysofmitochondrialcristaformation AT walterneupert evidencebasedhypothesisontheexistenceoftwopathwaysofmitochondrialcristaformation |