Assessment of genetic diversity in Amitostigma pinguiculum revealed with SRAP Markers(大花无柱兰遗传多样性的SRAP 标记分析)
大花无柱兰是一种珍稀兰科植物,具有一定的观赏和药用价值,但数量十分稀少,该物种亟待保护。本研究采用SRAP 分子标记技术,对10 个居群的115 份DNA 样品进行PCR 扩增,并开展遗传多样性分析。从81 对引物中筛选出9 个条带清晰、多态性好、重复性高的引物组合,共扩增得到305 条谱带。在物种水平上,多态性比率(PPB) 为100%,Nei’s 基因多样性指数(H) 为0.209 8,Shannon’s 指数(I) 为0.340 2;在居群水平上,PPB 为24.59%~52.13%,H 为0.079 6~0.165 5,I 为0.120 9~0.252 3。居群水平上,基因分化度(Gs...
Main Authors: | ZHOUMengying(周梦莹), TANGLujing(唐露静), HUANGMengmeng(黄檬檬), XUJialu(徐佳璐), LUKaijie(鲁锴杰), ZHUXin(朱欣), JIANGMing(蒋明) |
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Format: | Article |
Language: | zho |
Published: |
Zhejiang University Press
2019-11-01
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Series: | Zhejiang Daxue xuebao. Lixue ban |
Subjects: | |
Online Access: | https://doi.org/10.3785/j.issn.1008-9497.2019.06.014 |
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