Study of the microbiological composition of sludge during the cultivation of Chironomus larvae

Silt (nutrition medium) is the habitat of many microorganisms that adapt well to water and soil. By extended aeration, it partially comes out of the sewage. The nature of organic impurities determines the composition of such a substrate, so its leading indicators may vary. Such a nutrient medium is...

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Main Authors: L. P. Korol-Bezpala, I. F. Bezpalyi, А. P. Korol, L. V. Bondarenko
Format: Article
Language:English
Published: Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv 2023-04-01
Series:Ukrainian Journal of Veterinary and Agricultural Sciences
Subjects:
Online Access:https://ujvas.com.ua/index.php/journal/article/view/154
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author L. P. Korol-Bezpala
I. F. Bezpalyi
А. P. Korol
L. V. Bondarenko
author_facet L. P. Korol-Bezpala
I. F. Bezpalyi
А. P. Korol
L. V. Bondarenko
author_sort L. P. Korol-Bezpala
collection DOAJ
description Silt (nutrition medium) is the habitat of many microorganisms that adapt well to water and soil. By extended aeration, it partially comes out of the sewage. The nature of organic impurities determines the composition of such a substrate, so its leading indicators may vary. Such a nutrient medium is one of the favorable conditions for the growth and development of Chironomus larvae and various microflora. The article presents the results of studies of bacteriological crops of the substrate for Chironomus larvae to establish the microbiological composition of the nutrient medium under various pasteurization modes. It was found that in native sludge, the growth of a colony of bacteria of the family Pseudomonas spesialis is 80 %, Bacillus subtilis is 15 %, and Micrococcus luteus is 5 % of the total number of colony-forming organisms. It was also found that different modes of pasteurization lead to a decrease in the number of microorganisms in the nutrient medium. When pasteurized at 65 °C (long-term), the number of microorganisms decreased by 16 times. When pasteurized at 75 °C (short-term), the number of colony-forming organisms decreased by 22 times, and at 95 °C (instant), it decreased by 358 times compared with the native form of the nutrient medium.
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spelling doaj.art-e849c03064914a99a0b935a19862caee2023-07-26T11:58:43ZengStepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies LvivUkrainian Journal of Veterinary and Agricultural Sciences2617-61492023-04-016110210610.32718/ujvas6-1.16154Study of the microbiological composition of sludge during the cultivation of Chironomus larvaeL. P. Korol-Bezpala0I. F. Bezpalyi1А. P. Korol2L. V. Bondarenko3Bila Tserkva National Agrarian University, Pl. Soborna 8/1, Bila Tserkva, 09117, UkraineBila Tserkva National Agrarian University, Pl. Soborna 8/1, Bila Tserkva, 09117, UkraineBila Tserkva National Agrarian University, Pl. Soborna 8/1, Bila Tserkva, 09117, UkraineBila Tserkva National Agrarian University, Pl. Soborna 8/1, Bila Tserkva, 09117, UkraineSilt (nutrition medium) is the habitat of many microorganisms that adapt well to water and soil. By extended aeration, it partially comes out of the sewage. The nature of organic impurities determines the composition of such a substrate, so its leading indicators may vary. Such a nutrient medium is one of the favorable conditions for the growth and development of Chironomus larvae and various microflora. The article presents the results of studies of bacteriological crops of the substrate for Chironomus larvae to establish the microbiological composition of the nutrient medium under various pasteurization modes. It was found that in native sludge, the growth of a colony of bacteria of the family Pseudomonas spesialis is 80 %, Bacillus subtilis is 15 %, and Micrococcus luteus is 5 % of the total number of colony-forming organisms. It was also found that different modes of pasteurization lead to a decrease in the number of microorganisms in the nutrient medium. When pasteurized at 65 °C (long-term), the number of microorganisms decreased by 16 times. When pasteurized at 75 °C (short-term), the number of colony-forming organisms decreased by 22 times, and at 95 °C (instant), it decreased by 358 times compared with the native form of the nutrient medium.https://ujvas.com.ua/index.php/journal/article/view/154: sludge; bacteria; pasteurization modes; colony growth
spellingShingle L. P. Korol-Bezpala
I. F. Bezpalyi
А. P. Korol
L. V. Bondarenko
Study of the microbiological composition of sludge during the cultivation of Chironomus larvae
Ukrainian Journal of Veterinary and Agricultural Sciences
: sludge; bacteria; pasteurization modes; colony growth
title Study of the microbiological composition of sludge during the cultivation of Chironomus larvae
title_full Study of the microbiological composition of sludge during the cultivation of Chironomus larvae
title_fullStr Study of the microbiological composition of sludge during the cultivation of Chironomus larvae
title_full_unstemmed Study of the microbiological composition of sludge during the cultivation of Chironomus larvae
title_short Study of the microbiological composition of sludge during the cultivation of Chironomus larvae
title_sort study of the microbiological composition of sludge during the cultivation of chironomus larvae
topic : sludge; bacteria; pasteurization modes; colony growth
url https://ujvas.com.ua/index.php/journal/article/view/154
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AT ifbezpalyi studyofthemicrobiologicalcompositionofsludgeduringthecultivationofchironomuslarvae
AT apkorol studyofthemicrobiologicalcompositionofsludgeduringthecultivationofchironomuslarvae
AT lvbondarenko studyofthemicrobiologicalcompositionofsludgeduringthecultivationofchironomuslarvae