Molecular detection of Toxoplasma gondii in aborted fetuses of goats in Chattogram, Bangladesh
Background and Aim: Toxoplasma gondii is a protozoan parasite that is responsible for the major cause of congenital diseases, abortion, and stillbirth in humans and farm animals. Primary infection in pregnant goats due to T. gondii leads to abortion and significant economic losses in the livestock i...
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| Format: | Article |
| Language: | English |
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Veterinary World
2021-09-01
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| Series: | Veterinary World |
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| Online Access: | http://www.veterinaryworld.org/Vol.14/September-2021/13.pdf |
| _version_ | 1818358727408877568 |
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| author | Tanjila Hasan Abdul Mannan Delower Hossain Azizunnesa Rekha Md. Monir Hossan Mohammad Abdul Alim AHM Musleh Uddin |
| author_facet | Tanjila Hasan Abdul Mannan Delower Hossain Azizunnesa Rekha Md. Monir Hossan Mohammad Abdul Alim AHM Musleh Uddin |
| author_sort | Tanjila Hasan |
| collection | DOAJ |
| description | Background and Aim: Toxoplasma gondii is a protozoan parasite that is responsible for the major cause of congenital diseases, abortion, and stillbirth in humans and farm animals. Primary infection in pregnant goats due to T. gondii leads to abortion and significant economic losses in the livestock industry. Moreover, very few studies have been performed for the identification of T. gondii from aborted fetuses of goats. The study was conducted for the molecular identification of Toxoplasma gondii from aborted fetuses of goats in Chattogram, Bangladesh.
Materials and Methods: Twenty aborted fetuses of goats were collected from 52 farms in the study area. A nested polymerase chain reaction (PCR) assay targeting the B1 gene was performed, and a positive sample yield of 197 bp amplified DNA products consistent with T. gondii.
Results: The overall prevalence of toxoplasmosis in the aborted fetus of goats was 35.0%. Heart muscle, liver, brain, and placenta showed positive PCR results. The risk factors related to the does age, presence of cats in farms, and aborted fetus age were found to be statistically significant (p<0.05). Our results showed that T. gondii is a major possible causal factor for abortion and reproductive failure in goats. The high prevalence of T. gondii infection in aborted fetuses of goats revealed that T. gondii could be imperative in causing reproductive failure in goats.
Conclusion: Active or congenital toxoplasmosis was shown by the presence of T. gondii in fetal tissues, which is a matter of concern as this parasite has zoonotic significance and causes economic hazards to the livestock industry by causing various reproductive problems. Therefore, proper control measures and strategies are needed to reduce the rate of abortion in goats, ultimately saving the livestock industry. |
| first_indexed | 2024-12-13T20:33:36Z |
| format | Article |
| id | doaj.art-e84b2776532a4c6f8daef582546a2e52 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-13T20:33:36Z |
| publishDate | 2021-09-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-e84b2776532a4c6f8daef582546a2e522022-12-21T23:32:22ZengVeterinary WorldVeterinary World0972-89882231-09162021-09-011492386239110.14202/vetworld.2021.2386-2391Molecular detection of Toxoplasma gondii in aborted fetuses of goats in Chattogram, BangladeshTanjila Hasan0https://orcid.org/0000-0001-7001-991XAbdul Mannan1https://orcid.org/0000-0002-5145-9298Delower Hossain2https://orcid.org/0000-0001-9269-5353Azizunnesa Rekha3https://orcid.org/0000-0002-5809-4164Md. Monir Hossan4https://orcid.org/0000-0002-3745-8862Mohammad Abdul Alim5https://orcid.org/0000-0002-3945-6801AHM Musleh Uddin6https://orcid.org/0000-0002-4393-3711Department of Medicine and Surgery, Faculty of Veterinary Medicine, Chattogram Veterinary and Animal Sciences University, Chattogram 4225, Bangladesh.Teaching and Training Pet Hospital and Research Center, Chattogram Veterinary and Animal Sciences University, Chattogram 4225, Bangladesh.Department of Medicine and Public Health, Faculty of Animal Science and Veterinary Medicine, Sher-e-Bangla Agricultural University, Dhaka 1207, Bangladesh.Department of Medicine and Surgery, Faculty of Veterinary Medicine, Chattogram Veterinary and Animal Sciences University, Chattogram 4225, Bangladesh.Upazila Livestock Office, Department of Livestock Services, Farmgate, Dhaka 1215, Bangladesh.Department of Pathology and Parasitology, Faculty of Veterinary Medicine, Chattogram Veterinary and Animal Sciences University, Chattogram 4225, Bangladesh.Department of Surgery and Theriogenology, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet 3100, Bangladesh.Background and Aim: Toxoplasma gondii is a protozoan parasite that is responsible for the major cause of congenital diseases, abortion, and stillbirth in humans and farm animals. Primary infection in pregnant goats due to T. gondii leads to abortion and significant economic losses in the livestock industry. Moreover, very few studies have been performed for the identification of T. gondii from aborted fetuses of goats. The study was conducted for the molecular identification of Toxoplasma gondii from aborted fetuses of goats in Chattogram, Bangladesh. Materials and Methods: Twenty aborted fetuses of goats were collected from 52 farms in the study area. A nested polymerase chain reaction (PCR) assay targeting the B1 gene was performed, and a positive sample yield of 197 bp amplified DNA products consistent with T. gondii. Results: The overall prevalence of toxoplasmosis in the aborted fetus of goats was 35.0%. Heart muscle, liver, brain, and placenta showed positive PCR results. The risk factors related to the does age, presence of cats in farms, and aborted fetus age were found to be statistically significant (p<0.05). Our results showed that T. gondii is a major possible causal factor for abortion and reproductive failure in goats. The high prevalence of T. gondii infection in aborted fetuses of goats revealed that T. gondii could be imperative in causing reproductive failure in goats. Conclusion: Active or congenital toxoplasmosis was shown by the presence of T. gondii in fetal tissues, which is a matter of concern as this parasite has zoonotic significance and causes economic hazards to the livestock industry by causing various reproductive problems. Therefore, proper control measures and strategies are needed to reduce the rate of abortion in goats, ultimately saving the livestock industry.http://www.veterinaryworld.org/Vol.14/September-2021/13.pdfaborted fetusgoatnested polymerase chain reactiontoxoplasma gondii |
| spellingShingle | Tanjila Hasan Abdul Mannan Delower Hossain Azizunnesa Rekha Md. Monir Hossan Mohammad Abdul Alim AHM Musleh Uddin Molecular detection of Toxoplasma gondii in aborted fetuses of goats in Chattogram, Bangladesh Veterinary World aborted fetus goat nested polymerase chain reaction toxoplasma gondii |
| title | Molecular detection of Toxoplasma gondii in aborted fetuses of goats in Chattogram, Bangladesh |
| title_full | Molecular detection of Toxoplasma gondii in aborted fetuses of goats in Chattogram, Bangladesh |
| title_fullStr | Molecular detection of Toxoplasma gondii in aborted fetuses of goats in Chattogram, Bangladesh |
| title_full_unstemmed | Molecular detection of Toxoplasma gondii in aborted fetuses of goats in Chattogram, Bangladesh |
| title_short | Molecular detection of Toxoplasma gondii in aborted fetuses of goats in Chattogram, Bangladesh |
| title_sort | molecular detection of toxoplasma gondii in aborted fetuses of goats in chattogram bangladesh |
| topic | aborted fetus goat nested polymerase chain reaction toxoplasma gondii |
| url | http://www.veterinaryworld.org/Vol.14/September-2021/13.pdf |
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