The combined effects of Pseudomonas fluorescens CECT 844 and the black truffle co-inoculation on Pinus nigra seedlings
The inoculation of forest seedlings with mycorrhizal fungi and rhizobacteria can improve the morphology and physiology of the seedlings and benefit the reforestation of Mediterranean areas and the reintroduction of mycorrhizal fungal inocula into these areas. Pinus nigra subsp. salzmannii,a forest c...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Italian Society of Silviculture and Forest Ecology (SISEF)
2015-10-01
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| Series: | iForest - Biogeosciences and Forestry |
| Subjects: | |
| Online Access: | https://iforest.sisef.org/contents/?id=ifor1334-007 |
| _version_ | 1818081584433070080 |
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| author | Dominguez-Nuñez José A Medina M Berrocal-Lobo M Anriquez A Albanesi A |
| author_facet | Dominguez-Nuñez José A Medina M Berrocal-Lobo M Anriquez A Albanesi A |
| author_sort | Dominguez-Nuñez José A |
| collection | DOAJ |
| description | The inoculation of forest seedlings with mycorrhizal fungi and rhizobacteria can improve the morphology and physiology of the seedlings and benefit the reforestation of Mediterranean areas and the reintroduction of mycorrhizal fungal inocula into these areas. Pinus nigra subsp. salzmannii,a forest component of the Mediterranean natural ecosystems, is currently used in the reforestation of Mediterranean regions. Its roots are able to form an ectomycorrhizal symbiosis with the Ascomycetes fungus Tuber melanosporum Vitt., the black truffle. The ecological, economic and social values of this ectomycorrhizal fungus is well known. Previously, we demonstrated that the inoculation of Pinus halepensis seedlings with Pseudomonas fluorescens CECT 844 rhizobacteria and the black truffle T. melanosporum improved the plant growth and N absorption of the seedlings. Furthermore, the addition of P. fluorescens CECT 844 doubled the rate of mycorrhization of T. melanosporum. In the present work, P. nigra seedlings were produced in a nursery under well-watered conditions. We studied the morphophysiological response of these seedlings to a combined T. melanosporum and/or a rhizobacteria P. fluorescens CECT 844 inoculation. Five months after inoculation, the growth parameters (seedling height, basal diameter, and shoot and root dry weight), mycorrhizal colonization, water parameters (osmotic potential at both full and zero turgor and modulus of elasticity), and the total contents and concentrations of N, P, and K in the seedlings roots and shoots were measured. The root growth potentials were subsequently estimated. The addition of P. fluorescens CECT 844 did not significantly improve the mycorrhizal colonization by T. melanosporum on P. nigra seedlings. Additionally, the P. fluorescens inoculation caused few significant improvements in the growth and water parameters. Moreover, apparently opposing effects were observed between the two inoculations regarding the seedlings P absorption. We discuss whether P. fluorescens CECT 844 could act as a Mycorrhizal Helper Bacterium (MHB) through different mechanisms depending on the environmental conditions. |
| first_indexed | 2024-12-10T19:08:32Z |
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| id | doaj.art-e84d3139019b4d71a9919323ac9d10b2 |
| institution | Directory Open Access Journal |
| issn | 1971-7458 1971-7458 |
| language | English |
| last_indexed | 2024-12-10T19:08:32Z |
| publishDate | 2015-10-01 |
| publisher | Italian Society of Silviculture and Forest Ecology (SISEF) |
| record_format | Article |
| series | iForest - Biogeosciences and Forestry |
| spelling | doaj.art-e84d3139019b4d71a9919323ac9d10b22022-12-22T01:36:49ZengItalian Society of Silviculture and Forest Ecology (SISEF)iForest - Biogeosciences and Forestry1971-74581971-74582015-10-018162463010.3832/ifor1334-0071334The combined effects of Pseudomonas fluorescens CECT 844 and the black truffle co-inoculation on Pinus nigra seedlingsDominguez-Nuñez José A0Medina M1Berrocal-Lobo M2Anriquez A3Albanesi A4ETSI Mountains, Polytechnic University of Madrid, Av. Ciudad Universitaria s/n, E-28040 Madrid (Spain)ETSI Mountains, Polytechnic University of Madrid, Av. Ciudad Universitaria s/n, E-28040 Madrid (Spain)ETSI Mountains, Polytechnic University of Madrid, Av. Ciudad Universitaria s/n, E-28040 Madrid (Spain)Faculty of Agronomy and Agroindustries, Nacional University of Santiago de Estero, Av. Belgrano (S) 1912, Santiago del Estero (Argentina)Faculty of Agronomy and Agroindustries, Nacional University of Santiago de Estero, Av. Belgrano (S) 1912, Santiago del Estero (Argentina)The inoculation of forest seedlings with mycorrhizal fungi and rhizobacteria can improve the morphology and physiology of the seedlings and benefit the reforestation of Mediterranean areas and the reintroduction of mycorrhizal fungal inocula into these areas. Pinus nigra subsp. salzmannii,a forest component of the Mediterranean natural ecosystems, is currently used in the reforestation of Mediterranean regions. Its roots are able to form an ectomycorrhizal symbiosis with the Ascomycetes fungus Tuber melanosporum Vitt., the black truffle. The ecological, economic and social values of this ectomycorrhizal fungus is well known. Previously, we demonstrated that the inoculation of Pinus halepensis seedlings with Pseudomonas fluorescens CECT 844 rhizobacteria and the black truffle T. melanosporum improved the plant growth and N absorption of the seedlings. Furthermore, the addition of P. fluorescens CECT 844 doubled the rate of mycorrhization of T. melanosporum. In the present work, P. nigra seedlings were produced in a nursery under well-watered conditions. We studied the morphophysiological response of these seedlings to a combined T. melanosporum and/or a rhizobacteria P. fluorescens CECT 844 inoculation. Five months after inoculation, the growth parameters (seedling height, basal diameter, and shoot and root dry weight), mycorrhizal colonization, water parameters (osmotic potential at both full and zero turgor and modulus of elasticity), and the total contents and concentrations of N, P, and K in the seedlings roots and shoots were measured. The root growth potentials were subsequently estimated. The addition of P. fluorescens CECT 844 did not significantly improve the mycorrhizal colonization by T. melanosporum on P. nigra seedlings. Additionally, the P. fluorescens inoculation caused few significant improvements in the growth and water parameters. Moreover, apparently opposing effects were observed between the two inoculations regarding the seedlings P absorption. We discuss whether P. fluorescens CECT 844 could act as a Mycorrhizal Helper Bacterium (MHB) through different mechanisms depending on the environmental conditions.https://iforest.sisef.org/contents/?id=ifor1334-007RhizobacteriaBlack TruffleMycorrhizaMycorrhiza Helper Bacteria |
| spellingShingle | Dominguez-Nuñez José A Medina M Berrocal-Lobo M Anriquez A Albanesi A The combined effects of Pseudomonas fluorescens CECT 844 and the black truffle co-inoculation on Pinus nigra seedlings iForest - Biogeosciences and Forestry Rhizobacteria Black Truffle Mycorrhiza Mycorrhiza Helper Bacteria |
| title | The combined effects of Pseudomonas fluorescens CECT 844 and the black truffle co-inoculation on Pinus nigra seedlings |
| title_full | The combined effects of Pseudomonas fluorescens CECT 844 and the black truffle co-inoculation on Pinus nigra seedlings |
| title_fullStr | The combined effects of Pseudomonas fluorescens CECT 844 and the black truffle co-inoculation on Pinus nigra seedlings |
| title_full_unstemmed | The combined effects of Pseudomonas fluorescens CECT 844 and the black truffle co-inoculation on Pinus nigra seedlings |
| title_short | The combined effects of Pseudomonas fluorescens CECT 844 and the black truffle co-inoculation on Pinus nigra seedlings |
| title_sort | combined effects of pseudomonas fluorescens cect 844 and the black truffle co inoculation on pinus nigra seedlings |
| topic | Rhizobacteria Black Truffle Mycorrhiza Mycorrhiza Helper Bacteria |
| url | https://iforest.sisef.org/contents/?id=ifor1334-007 |
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