Correlation between <i>Peptacetobacter hiranonis</i>, the <i>baiCD</i> Gene, and Secondary Bile Acids in Dogs
Bile acid metabolism is a key pathway modulated by intestinal microbiota. <i>Peptacetobacter</i> (<i>Clostridium</i>) <i>hiranonis</i> has been described as the main species responsible for the conversion of primary into secondary fecal unconjugated bile acids (fU...
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| Format: | Article |
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MDPI AG
2024-01-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/14/2/216 |
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| author | Bruna Correa Lopes Chih-Chun Chen Chi-Hsuan Sung Patricia Eri Ishii Luis Fernando da Costa Medina Frederic P. Gaschen Jan S. Suchodolski Rachel Pilla |
| author_facet | Bruna Correa Lopes Chih-Chun Chen Chi-Hsuan Sung Patricia Eri Ishii Luis Fernando da Costa Medina Frederic P. Gaschen Jan S. Suchodolski Rachel Pilla |
| author_sort | Bruna Correa Lopes |
| collection | DOAJ |
| description | Bile acid metabolism is a key pathway modulated by intestinal microbiota. <i>Peptacetobacter</i> (<i>Clostridium</i>) <i>hiranonis</i> has been described as the main species responsible for the conversion of primary into secondary fecal unconjugated bile acids (fUBA) in dogs. This multi-step biochemical pathway is encoded by the bile acid-inducible (<i>bai</i>) operon. We aimed to assess the correlation between <i>P. hiranonis</i> abundance, the abundance of one specific gene of the <i>bai</i> operon (<i>baiCD</i>), and secondary fUBA concentrations. In this retrospective study, 133 fecal samples were analyzed from 24 dogs. The abundances of <i>P. hiranonis</i> and <i>baiCD</i> were determined using qPCR. The concentration of fUBA was measured by gas chromatography–mass spectrometry. The <i>baiCD</i> abundance exhibited a strong positive correlation with secondary fUBA (ρ = 0.7377, 95% CI (0.6461, 0.8084), <i>p</i> < 0.0001). Similarly, there was a strong correlation between <i>P. hiranonis</i> and secondary fUBA (ρ = 0.6658, 95% CI (0.5555, 0.7532), <i>p <</i> 0.0001). Animals displaying conversion of fUBA and lacking <i>P. hiranonis</i> were not observed. These results suggest <i>P. hiranonis</i> is the main converter of primary to secondary bile acids in dogs. |
| first_indexed | 2024-03-08T11:08:55Z |
| format | Article |
| id | doaj.art-e84e27ffef6d48a1bff1d41b410e9684 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-08T11:08:55Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-e84e27ffef6d48a1bff1d41b410e96842024-01-26T14:31:00ZengMDPI AGAnimals2076-26152024-01-0114221610.3390/ani14020216Correlation between <i>Peptacetobacter hiranonis</i>, the <i>baiCD</i> Gene, and Secondary Bile Acids in DogsBruna Correa Lopes0Chih-Chun Chen1Chi-Hsuan Sung2Patricia Eri Ishii3Luis Fernando da Costa Medina4Frederic P. Gaschen5Jan S. Suchodolski6Rachel Pilla7Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences, Texas A&M University, College Station, TX 77840, USAGastrointestinal Laboratory, Department of Small Animal Clinical Sciences, Texas A&M University, College Station, TX 77840, USAGastrointestinal Laboratory, Department of Small Animal Clinical Sciences, Texas A&M University, College Station, TX 77840, USAGastrointestinal Laboratory, Department of Small Animal Clinical Sciences, Texas A&M University, College Station, TX 77840, USAGastrointestinal Laboratory, Department of Small Animal Clinical Sciences, Texas A&M University, College Station, TX 77840, USADepartment of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USAGastrointestinal Laboratory, Department of Small Animal Clinical Sciences, Texas A&M University, College Station, TX 77840, USAGastrointestinal Laboratory, Department of Small Animal Clinical Sciences, Texas A&M University, College Station, TX 77840, USABile acid metabolism is a key pathway modulated by intestinal microbiota. <i>Peptacetobacter</i> (<i>Clostridium</i>) <i>hiranonis</i> has been described as the main species responsible for the conversion of primary into secondary fecal unconjugated bile acids (fUBA) in dogs. This multi-step biochemical pathway is encoded by the bile acid-inducible (<i>bai</i>) operon. We aimed to assess the correlation between <i>P. hiranonis</i> abundance, the abundance of one specific gene of the <i>bai</i> operon (<i>baiCD</i>), and secondary fUBA concentrations. In this retrospective study, 133 fecal samples were analyzed from 24 dogs. The abundances of <i>P. hiranonis</i> and <i>baiCD</i> were determined using qPCR. The concentration of fUBA was measured by gas chromatography–mass spectrometry. The <i>baiCD</i> abundance exhibited a strong positive correlation with secondary fUBA (ρ = 0.7377, 95% CI (0.6461, 0.8084), <i>p</i> < 0.0001). Similarly, there was a strong correlation between <i>P. hiranonis</i> and secondary fUBA (ρ = 0.6658, 95% CI (0.5555, 0.7532), <i>p <</i> 0.0001). Animals displaying conversion of fUBA and lacking <i>P. hiranonis</i> were not observed. These results suggest <i>P. hiranonis</i> is the main converter of primary to secondary bile acids in dogs.https://www.mdpi.com/2076-2615/14/2/216fecal unconjugated bile acidscanine<i>Clostridium hiranonis</i>primary bile acidsconversion of bile acidsbile acid metabolism |
| spellingShingle | Bruna Correa Lopes Chih-Chun Chen Chi-Hsuan Sung Patricia Eri Ishii Luis Fernando da Costa Medina Frederic P. Gaschen Jan S. Suchodolski Rachel Pilla Correlation between <i>Peptacetobacter hiranonis</i>, the <i>baiCD</i> Gene, and Secondary Bile Acids in Dogs Animals fecal unconjugated bile acids canine <i>Clostridium hiranonis</i> primary bile acids conversion of bile acids bile acid metabolism |
| title | Correlation between <i>Peptacetobacter hiranonis</i>, the <i>baiCD</i> Gene, and Secondary Bile Acids in Dogs |
| title_full | Correlation between <i>Peptacetobacter hiranonis</i>, the <i>baiCD</i> Gene, and Secondary Bile Acids in Dogs |
| title_fullStr | Correlation between <i>Peptacetobacter hiranonis</i>, the <i>baiCD</i> Gene, and Secondary Bile Acids in Dogs |
| title_full_unstemmed | Correlation between <i>Peptacetobacter hiranonis</i>, the <i>baiCD</i> Gene, and Secondary Bile Acids in Dogs |
| title_short | Correlation between <i>Peptacetobacter hiranonis</i>, the <i>baiCD</i> Gene, and Secondary Bile Acids in Dogs |
| title_sort | correlation between i peptacetobacter hiranonis i the i baicd i gene and secondary bile acids in dogs |
| topic | fecal unconjugated bile acids canine <i>Clostridium hiranonis</i> primary bile acids conversion of bile acids bile acid metabolism |
| url | https://www.mdpi.com/2076-2615/14/2/216 |
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