Characterization and optimization of clove oil-loaded nanomicelles for the possible topical use of bacterial infection-led atopic dermatitis
Abstract Background Atopic dermatitis is an abnormal skin condition that impacts a significant number of people in the US, with an estimated 9.6 million children and 16.5 million adults being affected by it. The study aimed to characterize and optimize clove oil-based nanomicelles for the possible t...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2023-10-01
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| Series: | Beni-Suef University Journal of Basic and Applied Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43088-023-00430-4 |
| _version_ | 1827633443759980544 |
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| author | Gulam Mustafa Rand Abdullah Almohsen Munira Motlaq Alotaibi Mohammed Majed Alotaibi Ruaa Majed Alotaibi Ahmed Farag El Kirdasy Farhan R. Khan Nahed S. Alharthi Abdulkarim S. Binshaya Faisal Alotaibi Md Salahuddin Ansari |
| author_facet | Gulam Mustafa Rand Abdullah Almohsen Munira Motlaq Alotaibi Mohammed Majed Alotaibi Ruaa Majed Alotaibi Ahmed Farag El Kirdasy Farhan R. Khan Nahed S. Alharthi Abdulkarim S. Binshaya Faisal Alotaibi Md Salahuddin Ansari |
| author_sort | Gulam Mustafa |
| collection | DOAJ |
| description | Abstract Background Atopic dermatitis is an abnormal skin condition that impacts a significant number of people in the US, with an estimated 9.6 million children and 16.5 million adults being affected by it. The study aimed to characterize and optimize clove oil-based nanomicelles for the possible topical use of bacterial infection-led atopic dermatitis. Clove oil-loaded nanomicelles were produced and carefully analyzed for vesicle diameter, polydispersity index (PDI), zeta potential, morphological attributes, entrapment efficiency, in vitro release, stability, dermatokinetic parameters, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging behavior and antibacterial activity. Different models, such as Korsmeyer, Higuchi, first order, and zero order were employed to evaluate the in vitro release from the formulations. Results The average size of the clove oil nanomicelles was found to be 100.15 nm with a PDI of 0.2104; they were observed to be darker against a light background. The evaluated mean zeta size was 121.3 nm, the zeta potential was − 15.31 mV. The inhibitory concentration 50 (IC50) of the formulation was 61.32 ± 0.98 μg/mL; clove oil was 73.56 ± 1.63 μg/mL, against ascorbic acid was 54.51 ± 0.79 μg/mL. Among the four models tested for in vitro release kinetics, the Korsmeyer Peppas model was followed by the nanomicelles formulation. Clove oil nanomicelles generated a higher concentration of 148.68 w/v on the skin epidermis within 1.5 h, whereas the conventional formulation exhibited 55.287 w/v. Moreover, clove oil nanomicelles generated a higher concentration of 125.84 µg/mL on the skin's dermis within 2 h, whereas the conventional formulation produced 68.263 µg/mL. The nanomicelles also inhibited bacterial growth within a 24-h period. Conclusions The study presents initial evidence regarding the potency of clove oil-based nanomicelles and their enhanced efficiency on the skin. Thus, the prepared formulation can further be studied and incorporated for the possible use against bacterial infection-led atopic dermatitis. Graphical abstract |
| first_indexed | 2024-03-09T14:59:33Z |
| format | Article |
| id | doaj.art-e84e6cd8bb6142759955a1792b7a9255 |
| institution | Directory Open Access Journal |
| issn | 2314-8543 |
| language | English |
| last_indexed | 2024-03-09T14:59:33Z |
| publishDate | 2023-10-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Beni-Suef University Journal of Basic and Applied Sciences |
| spelling | doaj.art-e84e6cd8bb6142759955a1792b7a92552023-11-26T13:59:15ZengSpringerOpenBeni-Suef University Journal of Basic and Applied Sciences2314-85432023-10-0112111610.1186/s43088-023-00430-4Characterization and optimization of clove oil-loaded nanomicelles for the possible topical use of bacterial infection-led atopic dermatitisGulam Mustafa0Rand Abdullah Almohsen1Munira Motlaq Alotaibi2Mohammed Majed Alotaibi3Ruaa Majed Alotaibi4Ahmed Farag El Kirdasy5Farhan R. Khan6Nahed S. Alharthi7Abdulkarim S. Binshaya8Faisal Alotaibi9Md Salahuddin Ansari10Department of Pharmaceutical Sciences, College of Pharmacy, Shaqra UniversityPharmD Intern, College of Pharmacy, Shaqra UniversityPharmD Intern, College of Pharmacy, Shaqra UniversityCommunity Pharmacist, Aldawaa PharmacyPharmD Intern, College of Pharmacy, Shaqra UniversityDepartment of Biochemistry, College of Veterinary Medicine, University of Sadat CityDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences AlQuwayiyah, Shaqra UniversityDepartment of Medical Laboratory Sciences. College of Applied Medical Sciences in Al-Kharj, Prince Sattam Bin Abdulaziz UniversityDepartment of Medical Laboratory Sciences. College of Applied Medical Sciences in Al-Kharj, Prince Sattam Bin Abdulaziz UniversityDepartment of Pharmacy Practice, College of Pharmacy, Shaqra UniversityDepartment of Pharmacy Practice, College of Pharmacy, Shaqra UniversityAbstract Background Atopic dermatitis is an abnormal skin condition that impacts a significant number of people in the US, with an estimated 9.6 million children and 16.5 million adults being affected by it. The study aimed to characterize and optimize clove oil-based nanomicelles for the possible topical use of bacterial infection-led atopic dermatitis. Clove oil-loaded nanomicelles were produced and carefully analyzed for vesicle diameter, polydispersity index (PDI), zeta potential, morphological attributes, entrapment efficiency, in vitro release, stability, dermatokinetic parameters, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging behavior and antibacterial activity. Different models, such as Korsmeyer, Higuchi, first order, and zero order were employed to evaluate the in vitro release from the formulations. Results The average size of the clove oil nanomicelles was found to be 100.15 nm with a PDI of 0.2104; they were observed to be darker against a light background. The evaluated mean zeta size was 121.3 nm, the zeta potential was − 15.31 mV. The inhibitory concentration 50 (IC50) of the formulation was 61.32 ± 0.98 μg/mL; clove oil was 73.56 ± 1.63 μg/mL, against ascorbic acid was 54.51 ± 0.79 μg/mL. Among the four models tested for in vitro release kinetics, the Korsmeyer Peppas model was followed by the nanomicelles formulation. Clove oil nanomicelles generated a higher concentration of 148.68 w/v on the skin epidermis within 1.5 h, whereas the conventional formulation exhibited 55.287 w/v. Moreover, clove oil nanomicelles generated a higher concentration of 125.84 µg/mL on the skin's dermis within 2 h, whereas the conventional formulation produced 68.263 µg/mL. The nanomicelles also inhibited bacterial growth within a 24-h period. Conclusions The study presents initial evidence regarding the potency of clove oil-based nanomicelles and their enhanced efficiency on the skin. Thus, the prepared formulation can further be studied and incorporated for the possible use against bacterial infection-led atopic dermatitis. Graphical abstracthttps://doi.org/10.1186/s43088-023-00430-4Clove oilAtopic dermatitisSyzygium aromaticumNanomicelles |
| spellingShingle | Gulam Mustafa Rand Abdullah Almohsen Munira Motlaq Alotaibi Mohammed Majed Alotaibi Ruaa Majed Alotaibi Ahmed Farag El Kirdasy Farhan R. Khan Nahed S. Alharthi Abdulkarim S. Binshaya Faisal Alotaibi Md Salahuddin Ansari Characterization and optimization of clove oil-loaded nanomicelles for the possible topical use of bacterial infection-led atopic dermatitis Beni-Suef University Journal of Basic and Applied Sciences Clove oil Atopic dermatitis Syzygium aromaticum Nanomicelles |
| title | Characterization and optimization of clove oil-loaded nanomicelles for the possible topical use of bacterial infection-led atopic dermatitis |
| title_full | Characterization and optimization of clove oil-loaded nanomicelles for the possible topical use of bacterial infection-led atopic dermatitis |
| title_fullStr | Characterization and optimization of clove oil-loaded nanomicelles for the possible topical use of bacterial infection-led atopic dermatitis |
| title_full_unstemmed | Characterization and optimization of clove oil-loaded nanomicelles for the possible topical use of bacterial infection-led atopic dermatitis |
| title_short | Characterization and optimization of clove oil-loaded nanomicelles for the possible topical use of bacterial infection-led atopic dermatitis |
| title_sort | characterization and optimization of clove oil loaded nanomicelles for the possible topical use of bacterial infection led atopic dermatitis |
| topic | Clove oil Atopic dermatitis Syzygium aromaticum Nanomicelles |
| url | https://doi.org/10.1186/s43088-023-00430-4 |
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