Postmortem Metabolism and Pork Quality Development Are Affected by Electrical Stimulation across Three Genetic Lines
Variations in postmortem metabolism in muscle impact pork quality development. Curiously, some genetic lines are more refractile to adverse pork quality development than others and may regulate energy metabolism differently. The aim of this study was to challenge pork carcasses from different geneti...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/16/2599 |
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| author | Matthew D. Spires Jocelyn S. Bodmer Mariane Beline Jordan C. Wicks Morgan D. Zumbaugh Tim Hao Shi Brian T. Reichert Allan P. Schinckel Alan L. Grant David E. Gerrard |
| author_facet | Matthew D. Spires Jocelyn S. Bodmer Mariane Beline Jordan C. Wicks Morgan D. Zumbaugh Tim Hao Shi Brian T. Reichert Allan P. Schinckel Alan L. Grant David E. Gerrard |
| author_sort | Matthew D. Spires |
| collection | DOAJ |
| description | Variations in postmortem metabolism in muscle impact pork quality development. Curiously, some genetic lines are more refractile to adverse pork quality development than others and may regulate energy metabolism differently. The aim of this study was to challenge pork carcasses from different genetic populations with electrical stimulation (ES) to determine how postmortem metabolism varies with genetic line and explore control points that reside in glycolysis in dying muscle. Three genetic populations (GP) were subjected to ES (100 V or 200 V, 13 pulses, 2 s on/2 s off) at 15- or 25-min post-exsanguination, or no stimulation (NS). Genetic population affected relative muscle relative abundance of different myosin heavy chains, glycogen, G6P, and lactate concentrations. Genetic lines responded similarly to ES, but a comparison of ES treatment groups revealed a trend for an interaction between voltage, time of ES, and time postmortem. Higher voltage accelerated pH decline at 20 min up to 60 min postmortem. Trends in color and firmness scores and L* values were consistent with pH and metabolite data. These data show that genetic populations respond differently to postmortem perturbation by altering glycolytic flux and suggest differences in postmortem glycolysis may be partially responsible for differences in meat quality between genetic populations, though not entirely. |
| first_indexed | 2024-03-11T00:12:01Z |
| format | Article |
| id | doaj.art-e84eabfe6c184ea9967f4423611c9d93 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T00:12:01Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-e84eabfe6c184ea9967f4423611c9d932023-11-18T23:57:30ZengMDPI AGAnimals2076-26152023-08-011316259910.3390/ani13162599Postmortem Metabolism and Pork Quality Development Are Affected by Electrical Stimulation across Three Genetic LinesMatthew D. Spires0Jocelyn S. Bodmer1Mariane Beline2Jordan C. Wicks3Morgan D. Zumbaugh4Tim Hao Shi5Brian T. Reichert6Allan P. Schinckel7Alan L. Grant8David E. Gerrard9Department of Animal Sciences, Purdue University, West Lafayette, IN 47907, USASchool of Animal and Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USASchool of Animal and Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USASchool of Animal and Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USADepartment of Animal Sciences and Industry, Kansas State University, Manhattan, KS 66506, USASchool of Animal and Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USADepartment of Animal Sciences, Purdue University, West Lafayette, IN 47907, USADepartment of Animal Sciences, Purdue University, West Lafayette, IN 47907, USASchool of Animal and Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USASchool of Animal and Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USAVariations in postmortem metabolism in muscle impact pork quality development. Curiously, some genetic lines are more refractile to adverse pork quality development than others and may regulate energy metabolism differently. The aim of this study was to challenge pork carcasses from different genetic populations with electrical stimulation (ES) to determine how postmortem metabolism varies with genetic line and explore control points that reside in glycolysis in dying muscle. Three genetic populations (GP) were subjected to ES (100 V or 200 V, 13 pulses, 2 s on/2 s off) at 15- or 25-min post-exsanguination, or no stimulation (NS). Genetic population affected relative muscle relative abundance of different myosin heavy chains, glycogen, G6P, and lactate concentrations. Genetic lines responded similarly to ES, but a comparison of ES treatment groups revealed a trend for an interaction between voltage, time of ES, and time postmortem. Higher voltage accelerated pH decline at 20 min up to 60 min postmortem. Trends in color and firmness scores and L* values were consistent with pH and metabolite data. These data show that genetic populations respond differently to postmortem perturbation by altering glycolytic flux and suggest differences in postmortem glycolysis may be partially responsible for differences in meat quality between genetic populations, though not entirely.https://www.mdpi.com/2076-2615/13/16/2599geneticsglycolysisglycogenpHpork quality |
| spellingShingle | Matthew D. Spires Jocelyn S. Bodmer Mariane Beline Jordan C. Wicks Morgan D. Zumbaugh Tim Hao Shi Brian T. Reichert Allan P. Schinckel Alan L. Grant David E. Gerrard Postmortem Metabolism and Pork Quality Development Are Affected by Electrical Stimulation across Three Genetic Lines Animals genetics glycolysis glycogen pH pork quality |
| title | Postmortem Metabolism and Pork Quality Development Are Affected by Electrical Stimulation across Three Genetic Lines |
| title_full | Postmortem Metabolism and Pork Quality Development Are Affected by Electrical Stimulation across Three Genetic Lines |
| title_fullStr | Postmortem Metabolism and Pork Quality Development Are Affected by Electrical Stimulation across Three Genetic Lines |
| title_full_unstemmed | Postmortem Metabolism and Pork Quality Development Are Affected by Electrical Stimulation across Three Genetic Lines |
| title_short | Postmortem Metabolism and Pork Quality Development Are Affected by Electrical Stimulation across Three Genetic Lines |
| title_sort | postmortem metabolism and pork quality development are affected by electrical stimulation across three genetic lines |
| topic | genetics glycolysis glycogen pH pork quality |
| url | https://www.mdpi.com/2076-2615/13/16/2599 |
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