In Situ Analysis of DNA-Protein Complex Formation upon Radiation-Induced DNA Damage
The importance of determining at the cellular level the formation of DNA−protein complexes after radiation-induced lesions to DNA is outlined by the evidence that such interactions represent one of the first steps of the cellular response to DNA damage. These complexes are formed through r...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/20/22/5736 |
| _version_ | 1811216477503619072 |
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| author | Giulio Ticli Ennio Prosperi |
| author_facet | Giulio Ticli Ennio Prosperi |
| author_sort | Giulio Ticli |
| collection | DOAJ |
| description | The importance of determining at the cellular level the formation of DNA−protein complexes after radiation-induced lesions to DNA is outlined by the evidence that such interactions represent one of the first steps of the cellular response to DNA damage. These complexes are formed through recruitment at the sites of the lesion, of proteins deputed to signal the presence of DNA damage, and of DNA repair factors necessary to remove it. Investigating the formation of such complexes has provided, and will probably continue to, relevant information about molecular mechanisms and spatiotemporal dynamics of the processes that constitute the first barrier of cell defense against genome instability and related diseases. In this review, we will summarize and discuss the use of in situ procedures to detect the formation of DNA-protein complexes after radiation-induced DNA damage. This type of analysis provides important information on the spatial localization and temporal resolution of the formation of such complexes, at the single-cell level, allowing the study of heterogeneous cell populations. |
| first_indexed | 2024-04-12T06:39:45Z |
| format | Article |
| id | doaj.art-e85389bb68ac49cdbff8c263afd28312 |
| institution | Directory Open Access Journal |
| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-12T06:39:45Z |
| publishDate | 2019-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-e85389bb68ac49cdbff8c263afd283122022-12-22T03:43:45ZengMDPI AGInternational Journal of Molecular Sciences1422-00672019-11-012022573610.3390/ijms20225736ijms20225736In Situ Analysis of DNA-Protein Complex Formation upon Radiation-Induced DNA DamageGiulio Ticli0Ennio Prosperi1Istituto di Genetica Molecolare “Luca Cavalli Sforza”, Consiglio Nazionale delle Ricerche (CNR), 27100 Pavia, ItalyIstituto di Genetica Molecolare “Luca Cavalli Sforza”, Consiglio Nazionale delle Ricerche (CNR), 27100 Pavia, ItalyThe importance of determining at the cellular level the formation of DNA−protein complexes after radiation-induced lesions to DNA is outlined by the evidence that such interactions represent one of the first steps of the cellular response to DNA damage. These complexes are formed through recruitment at the sites of the lesion, of proteins deputed to signal the presence of DNA damage, and of DNA repair factors necessary to remove it. Investigating the formation of such complexes has provided, and will probably continue to, relevant information about molecular mechanisms and spatiotemporal dynamics of the processes that constitute the first barrier of cell defense against genome instability and related diseases. In this review, we will summarize and discuss the use of in situ procedures to detect the formation of DNA-protein complexes after radiation-induced DNA damage. This type of analysis provides important information on the spatial localization and temporal resolution of the formation of such complexes, at the single-cell level, allowing the study of heterogeneous cell populations.https://www.mdpi.com/1422-0067/20/22/5736dna damagedna repairdna-interacting proteinsin situ analysisimmunofluorescence detectionlive-cell imaging |
| spellingShingle | Giulio Ticli Ennio Prosperi In Situ Analysis of DNA-Protein Complex Formation upon Radiation-Induced DNA Damage International Journal of Molecular Sciences dna damage dna repair dna-interacting proteins in situ analysis immunofluorescence detection live-cell imaging |
| title | In Situ Analysis of DNA-Protein Complex Formation upon Radiation-Induced DNA Damage |
| title_full | In Situ Analysis of DNA-Protein Complex Formation upon Radiation-Induced DNA Damage |
| title_fullStr | In Situ Analysis of DNA-Protein Complex Formation upon Radiation-Induced DNA Damage |
| title_full_unstemmed | In Situ Analysis of DNA-Protein Complex Formation upon Radiation-Induced DNA Damage |
| title_short | In Situ Analysis of DNA-Protein Complex Formation upon Radiation-Induced DNA Damage |
| title_sort | in situ analysis of dna protein complex formation upon radiation induced dna damage |
| topic | dna damage dna repair dna-interacting proteins in situ analysis immunofluorescence detection live-cell imaging |
| url | https://www.mdpi.com/1422-0067/20/22/5736 |
| work_keys_str_mv | AT giulioticli insituanalysisofdnaproteincomplexformationuponradiationinduceddnadamage AT ennioprosperi insituanalysisofdnaproteincomplexformationuponradiationinduceddnadamage |