Two Novel Alliin Lyase (Alliinase) Genes from Twisted-Leaf Garlic (Allium obliquum) and Mountain Garlic (Allium senescens var. montanum)
Alliinase (Alliin lyase EC 4.4.1.4), a pyridoxal phosphate-dependent lyase, represents one of the major protein components of Allium species. The enzyme is a homodimeric glycoprotein and catalyzes the synthesis of allicin (diallyl thiosulfinate, a biologically active compound), pyruvate, and ammonia...
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| Language: | English |
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AcademicPres
2011-11-01
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| Series: | Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
| Online Access: | http://www.notulaebotanicae.ro/index.php/nbha/article/view/6355 |
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| author | Bogdan DRUGĂ Dana ŞUTEU Oana ROSCA-CASIAN Marcel PÂRVU Nicolae DRAGOŞ |
| author_facet | Bogdan DRUGĂ Dana ŞUTEU Oana ROSCA-CASIAN Marcel PÂRVU Nicolae DRAGOŞ |
| author_sort | Bogdan DRUGĂ |
| collection | DOAJ |
| description | Alliinase (Alliin lyase EC 4.4.1.4), a pyridoxal phosphate-dependent lyase, represents one of the major protein components of Allium species. The enzyme is a homodimeric glycoprotein and catalyzes the synthesis of allicin (diallyl thiosulfinate, a biologically active compound), pyruvate, and ammonia starting from the specific non-protein sulfur-containing amino acid alliin ((+S)-allyl-L-cysteine sulfoxide). Using newly developed specific primers two new alliinase genes from Allium obliquum and Allium senescens ssp. montanum were amplified and sequenced, as well as their homologs, from Allium fistulosum and Allium schoeonoprasum. The G+C content of the alliinase region ranges between that of other dicot plants and that reported in monocot cereal plants, in all four species. Investigations of gene expression revealed a significantly higher enzyme expression level in bulbs than in leaves in all four taxa. The deduced alliinase sequences displayed a high variability among different species, since the lowest sequence similarity was found to be 55.5% between Allium senescens var. montanum and Allium cepa, while the highest similarity is 77.5%, between Allium senescens var. montanum and Allium fistulosum. Leucine is the most common amino acid in all four alliinases, while cysteine is also more frequent that in other enzymes, suggesting a high stability of the molecules due to the possible disulfide bonds.<br /> |
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| id | doaj.art-e855f19d39134118968b840f945d5927 |
| institution | Directory Open Access Journal |
| issn | 0255-965X 1842-4309 |
| language | English |
| last_indexed | 2024-12-21T10:17:39Z |
| publishDate | 2011-11-01 |
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| series | Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
| spelling | doaj.art-e855f19d39134118968b840f945d59272022-12-21T19:07:33ZengAcademicPresNotulae Botanicae Horti Agrobotanici Cluj-Napoca0255-965X1842-43092011-11-0139229329810.15835/nbha39263556830Two Novel Alliin Lyase (Alliinase) Genes from Twisted-Leaf Garlic (Allium obliquum) and Mountain Garlic (Allium senescens var. montanum)Bogdan DRUGĂ0Dana ŞUTEU1Oana ROSCA-CASIAN2Marcel PÂRVU3Nicolae DRAGOŞ4Institute of Biological Research, 48 Republicii Street, 400015, Cluj-NapocaInstitute of Biological Research, 48 Republicii Street, 400015, Cluj-NapocaBabeş-Bolyai University, Faculty of Biology and Geology, „Alexandru Borza” Botanical Garden, 42 Republicii Street, 400015, Cluj-NapocaBabeş-Bolyai University, Faculty of Biology and Geology, „Alexandru Borza” Botanical Garden, 42 Republicii Street, 400015, Cluj-NapocaInstitute of Biological Research, 48 Republicii Street, 400015, Cluj-NapocaAlliinase (Alliin lyase EC 4.4.1.4), a pyridoxal phosphate-dependent lyase, represents one of the major protein components of Allium species. The enzyme is a homodimeric glycoprotein and catalyzes the synthesis of allicin (diallyl thiosulfinate, a biologically active compound), pyruvate, and ammonia starting from the specific non-protein sulfur-containing amino acid alliin ((+S)-allyl-L-cysteine sulfoxide). Using newly developed specific primers two new alliinase genes from Allium obliquum and Allium senescens ssp. montanum were amplified and sequenced, as well as their homologs, from Allium fistulosum and Allium schoeonoprasum. The G+C content of the alliinase region ranges between that of other dicot plants and that reported in monocot cereal plants, in all four species. Investigations of gene expression revealed a significantly higher enzyme expression level in bulbs than in leaves in all four taxa. The deduced alliinase sequences displayed a high variability among different species, since the lowest sequence similarity was found to be 55.5% between Allium senescens var. montanum and Allium cepa, while the highest similarity is 77.5%, between Allium senescens var. montanum and Allium fistulosum. Leucine is the most common amino acid in all four alliinases, while cysteine is also more frequent that in other enzymes, suggesting a high stability of the molecules due to the possible disulfide bonds.<br />http://www.notulaebotanicae.ro/index.php/nbha/article/view/6355 |
| spellingShingle | Bogdan DRUGĂ Dana ŞUTEU Oana ROSCA-CASIAN Marcel PÂRVU Nicolae DRAGOŞ Two Novel Alliin Lyase (Alliinase) Genes from Twisted-Leaf Garlic (Allium obliquum) and Mountain Garlic (Allium senescens var. montanum) Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
| title | Two Novel Alliin Lyase (Alliinase) Genes from Twisted-Leaf Garlic (Allium obliquum) and Mountain Garlic (Allium senescens var. montanum) |
| title_full | Two Novel Alliin Lyase (Alliinase) Genes from Twisted-Leaf Garlic (Allium obliquum) and Mountain Garlic (Allium senescens var. montanum) |
| title_fullStr | Two Novel Alliin Lyase (Alliinase) Genes from Twisted-Leaf Garlic (Allium obliquum) and Mountain Garlic (Allium senescens var. montanum) |
| title_full_unstemmed | Two Novel Alliin Lyase (Alliinase) Genes from Twisted-Leaf Garlic (Allium obliquum) and Mountain Garlic (Allium senescens var. montanum) |
| title_short | Two Novel Alliin Lyase (Alliinase) Genes from Twisted-Leaf Garlic (Allium obliquum) and Mountain Garlic (Allium senescens var. montanum) |
| title_sort | two novel alliin lyase alliinase genes from twisted leaf garlic allium obliquum and mountain garlic allium senescens var montanum |
| url | http://www.notulaebotanicae.ro/index.php/nbha/article/view/6355 |
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