Alterations of gut microbiota in patients with active pulmonary tuberculosis in China: a pilot study
Background: The aim of this study was to identify the differences in diversity, composition, and function of the gut microbiota between tuberculosis (TB) patients and healthy controls (HCs).Methods: A cross-sectional study was conducted in three cities of China. Stool samples from 94 treatment-naive...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-10-01
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| Series: | International Journal of Infectious Diseases |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1201971221006974 |
| _version_ | 1818826812297314304 |
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| author | Wenpei Shi Yi Hu Zhu Ning Fan Xia Meiying Wu Yue O.O. Hu Cheng Chen Stefanie Prast-Nielsen Biao Xu |
| author_facet | Wenpei Shi Yi Hu Zhu Ning Fan Xia Meiying Wu Yue O.O. Hu Cheng Chen Stefanie Prast-Nielsen Biao Xu |
| author_sort | Wenpei Shi |
| collection | DOAJ |
| description | Background: The aim of this study was to identify the differences in diversity, composition, and function of the gut microbiota between tuberculosis (TB) patients and healthy controls (HCs).Methods: A cross-sectional study was conducted in three cities of China. Stool samples from 94 treatment-naive TB patients and 62 HCs were analyzed by 16S rRNA gene sequencing. TB patients were further divided into antibiotic-free and antibiotic-exposure according to their use of non-specific antibiotics before the TB diagnosis.Results: Compared with HCs, antibiotic-free TB patients presented a different gut microbial community (P < 0.005) and decreased Shannon diversity (P < 0.005). Among TB patients, the relative abundances of short-chain fatty acid (SCFA)-producing genera such as Lachnospiraceae ND3007 group (log2(FC) = −2.74) were lower, while several conditional pathogen-related genera such as Enterococcus (log2(FC) = 12.05) and Rothia (log2(FC) = 6.322) were at higher levels. In addition, 41% of patients received antibiotics before TB diagnosis. Antibiotic exposure was correlated with an additional reduction in α diversity and depletion of SCFA-producing bacteria. Microbial functional analysis revealed that the biosynthesis capacity of amino acids and fatty acids was lower among TB patients compared to HCs.Conclusions: Significant alterations in gut microbiota composition and metabolic pathways of TB patients were observed. Antibiotic exposure could alter the gut microbiota of TB patients, which should be considered in anti-TB treatment. |
| first_indexed | 2024-12-19T00:33:36Z |
| format | Article |
| id | doaj.art-e858baf020ca45918a443bed2f50ca8f |
| institution | Directory Open Access Journal |
| issn | 1201-9712 |
| language | English |
| last_indexed | 2024-12-19T00:33:36Z |
| publishDate | 2021-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Infectious Diseases |
| spelling | doaj.art-e858baf020ca45918a443bed2f50ca8f2022-12-21T20:44:57ZengElsevierInternational Journal of Infectious Diseases1201-97122021-10-01111313321Alterations of gut microbiota in patients with active pulmonary tuberculosis in China: a pilot studyWenpei Shi0Yi Hu1Zhu Ning2Fan Xia3Meiying Wu4Yue O.O. Hu5Cheng Chen6Stefanie Prast-Nielsen7Biao Xu8Department of Epidemiology, School of Public Health and Key Laboratory of Public Health Safety (Ministry of Education), Fudan University, Shanghai, ChinaDepartment of Epidemiology, School of Public Health and Key Laboratory of Public Health Safety (Ministry of Education), Fudan University, Shanghai, ChinaZigong City Center for Disease Control and Prevention, Zigong City, Sichuan, ChinaDepartment of Tuberculosis, 905th Hospital of PLA Navy, Shanghai, ChinaDepartment of Clinical Laboratory, The Fifth People's Hospital of Suzhou(The Affiliated Infectious Diseases Hospital of Soochow University), Suzhou City, Jiangsu, ChinaCenter for Translational Microbiome Research (CTMR), Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, SwedenJiangsu Provincial Centre for Disease Control and Prevention, Nanjing, Jiangsu, ChinaCenter for Translational Microbiome Research (CTMR), Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, SwedenDepartment of Epidemiology, School of Public Health and Key Laboratory of Public Health Safety (Ministry of Education), Fudan University, Shanghai, China; Department of Public Health Sciences, Karolinska Institutet, Stockholm, Sweden; Corresponding author: Biao Xu, Department of Epidemiology, School of Public Health and Key Laboratory of Public Health Safety (Ministry of Education), Fudan University, Dongan Road No. 130, Shanghai, China. Tel: +86-21-54237710Background: The aim of this study was to identify the differences in diversity, composition, and function of the gut microbiota between tuberculosis (TB) patients and healthy controls (HCs).Methods: A cross-sectional study was conducted in three cities of China. Stool samples from 94 treatment-naive TB patients and 62 HCs were analyzed by 16S rRNA gene sequencing. TB patients were further divided into antibiotic-free and antibiotic-exposure according to their use of non-specific antibiotics before the TB diagnosis.Results: Compared with HCs, antibiotic-free TB patients presented a different gut microbial community (P < 0.005) and decreased Shannon diversity (P < 0.005). Among TB patients, the relative abundances of short-chain fatty acid (SCFA)-producing genera such as Lachnospiraceae ND3007 group (log2(FC) = −2.74) were lower, while several conditional pathogen-related genera such as Enterococcus (log2(FC) = 12.05) and Rothia (log2(FC) = 6.322) were at higher levels. In addition, 41% of patients received antibiotics before TB diagnosis. Antibiotic exposure was correlated with an additional reduction in α diversity and depletion of SCFA-producing bacteria. Microbial functional analysis revealed that the biosynthesis capacity of amino acids and fatty acids was lower among TB patients compared to HCs.Conclusions: Significant alterations in gut microbiota composition and metabolic pathways of TB patients were observed. Antibiotic exposure could alter the gut microbiota of TB patients, which should be considered in anti-TB treatment.http://www.sciencedirect.com/science/article/pii/S1201971221006974TuberculosisGut microbiota16S rRNA gene sequencingAntibiotic |
| spellingShingle | Wenpei Shi Yi Hu Zhu Ning Fan Xia Meiying Wu Yue O.O. Hu Cheng Chen Stefanie Prast-Nielsen Biao Xu Alterations of gut microbiota in patients with active pulmonary tuberculosis in China: a pilot study International Journal of Infectious Diseases Tuberculosis Gut microbiota 16S rRNA gene sequencing Antibiotic |
| title | Alterations of gut microbiota in patients with active pulmonary tuberculosis in China: a pilot study |
| title_full | Alterations of gut microbiota in patients with active pulmonary tuberculosis in China: a pilot study |
| title_fullStr | Alterations of gut microbiota in patients with active pulmonary tuberculosis in China: a pilot study |
| title_full_unstemmed | Alterations of gut microbiota in patients with active pulmonary tuberculosis in China: a pilot study |
| title_short | Alterations of gut microbiota in patients with active pulmonary tuberculosis in China: a pilot study |
| title_sort | alterations of gut microbiota in patients with active pulmonary tuberculosis in china a pilot study |
| topic | Tuberculosis Gut microbiota 16S rRNA gene sequencing Antibiotic |
| url | http://www.sciencedirect.com/science/article/pii/S1201971221006974 |
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