New Source of 3D Chitin Scaffolds: The Red Sea Demosponge <i>Pseudoceratina arabica</i> (Pseudoceratinidae, Verongiida)
The bioactive bromotyrosine-derived alkaloids and unique morphologically-defined fibrous skeleton of chitin origin have been found recently in marine demosponges of the order Verongiida. The sophisticated three-dimensional (3D) structure of skeletal chitinous scaffolds supported their use in biomedi...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-02-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/17/2/92 |
| _version_ | 1818036841292496896 |
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| author | Lamiaa A. Shaala Hani Z. Asfour Diaa T. A. Youssef Sonia Żółtowska-Aksamitowska Marcin Wysokowski Mikhail Tsurkan Roberta Galli Heike Meissner Iaroslav Petrenko Konstantin Tabachnick Viatcheslav N. Ivanenko Nicole Bechmann Lyubov V. Muzychka Oleg B. Smolii Rajko Martinović Yvonne Joseph Teofil Jesionowski Hermann Ehrlich |
| author_facet | Lamiaa A. Shaala Hani Z. Asfour Diaa T. A. Youssef Sonia Żółtowska-Aksamitowska Marcin Wysokowski Mikhail Tsurkan Roberta Galli Heike Meissner Iaroslav Petrenko Konstantin Tabachnick Viatcheslav N. Ivanenko Nicole Bechmann Lyubov V. Muzychka Oleg B. Smolii Rajko Martinović Yvonne Joseph Teofil Jesionowski Hermann Ehrlich |
| author_sort | Lamiaa A. Shaala |
| collection | DOAJ |
| description | The bioactive bromotyrosine-derived alkaloids and unique morphologically-defined fibrous skeleton of chitin origin have been found recently in marine demosponges of the order Verongiida. The sophisticated three-dimensional (3D) structure of skeletal chitinous scaffolds supported their use in biomedicine, tissue engineering as well as in diverse modern technologies. The goal of this study was the screening of new species of the order Verongiida to find another renewable source of naturally prefabricated 3D chitinous scaffolds. Special attention was paid to demosponge species, which could be farmed on large scale using marine aquaculture methods. In this study, the demosponge <i>Pseudoceratina arabica</i> collected in the coastal waters of the Egyptian Red Sea was examined as a potential source of chitin for the first time. Various bioanalytical tools including scanning electron microscopy (SEM), fluorescence microscopy, FTIR analysis, Calcofluor white staining, electrospray ionization mass spectrometry (ESI-MS), as well as a chitinase digestion assay were successfully used to confirm the discovery of α-chitin within the skeleton of <i>P. arabica</i>. The current finding should make an important contribution to the field of application of this verongiid sponge as a novel renewable source of biologically-active metabolites and chitin, which are important for development of the blue biotechnology especially in marine oriented biomedicine. |
| first_indexed | 2024-12-10T07:17:21Z |
| format | Article |
| id | doaj.art-e85c5398929f490bbea29fa5d5fc1f65 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-12-10T07:17:21Z |
| publishDate | 2019-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-e85c5398929f490bbea29fa5d5fc1f652022-12-22T01:57:55ZengMDPI AGMarine Drugs1660-33972019-02-011729210.3390/md17020092md17020092New Source of 3D Chitin Scaffolds: The Red Sea Demosponge <i>Pseudoceratina arabica</i> (Pseudoceratinidae, Verongiida)Lamiaa A. Shaala0Hani Z. Asfour1Diaa T. A. Youssef2Sonia Żółtowska-Aksamitowska3Marcin Wysokowski4Mikhail Tsurkan5Roberta Galli6Heike Meissner7Iaroslav Petrenko8Konstantin Tabachnick9Viatcheslav N. Ivanenko10Nicole Bechmann11Lyubov V. Muzychka12Oleg B. Smolii13Rajko Martinović14Yvonne Joseph15Teofil Jesionowski16Hermann Ehrlich17Natural Products Unit, King Fahd Medical Research Centre, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Medical Parasitology, Faculty of Medicine, Princess Al-Jawhara Center of Excellence in Research of Hereditary Disorders, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Natural Products, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi ArabiaInstitute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Poznan 60965, PolandInstitute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Poznan 60965, PolandLeibniz Institute of Polymer Research Dresden, Dresden 01069, GermanyClinical Sensoring and Monitoring, Department of Anesthesiology and Intensive Care Medicine, Faculty of Medicine, Technische Universität Dresden, Dresden 01307, GermanyDepartment of Prosthetic Dentistry, Faculty of Medicine, Technische Universität Dresden, Dresden 01307, GermanyInstitute of Electronics and Sensor Materials, Technische Universität Bergakademie-Freiberg, Freiberg 09599, GermanyP.P. Shirshov Institute of Oceanology, Russian Academy of Sciences, Moscow 117997, RussiaDepartment of Invertebrate Zoology, Biological Faculty, Lomonosov Moscow State University, Moscow 119992, RussiaInstitute of Clinical Chemistry and Laboratory Medicine, University Hospital Carl Gustav Carus at the Technische Universität Dresden, Dresden 01307, GermanyV.P. Kukhar Institute of Bioorganic Chemistry and Petrochemistry, National Academy of Science of Ukraine, Kiev 02094, UkraineV.P. Kukhar Institute of Bioorganic Chemistry and Petrochemistry, National Academy of Science of Ukraine, Kiev 02094, UkraineInstitute of Marine Biology, University of Montenegro, Kotor 85330, MontenegroInstitute of Electronics and Sensor Materials, Technische Universität Bergakademie-Freiberg, Freiberg 09599, GermanyInstitute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Poznan 60965, PolandInstitute of Electronics and Sensor Materials, Technische Universität Bergakademie-Freiberg, Freiberg 09599, GermanyThe bioactive bromotyrosine-derived alkaloids and unique morphologically-defined fibrous skeleton of chitin origin have been found recently in marine demosponges of the order Verongiida. The sophisticated three-dimensional (3D) structure of skeletal chitinous scaffolds supported their use in biomedicine, tissue engineering as well as in diverse modern technologies. The goal of this study was the screening of new species of the order Verongiida to find another renewable source of naturally prefabricated 3D chitinous scaffolds. Special attention was paid to demosponge species, which could be farmed on large scale using marine aquaculture methods. In this study, the demosponge <i>Pseudoceratina arabica</i> collected in the coastal waters of the Egyptian Red Sea was examined as a potential source of chitin for the first time. Various bioanalytical tools including scanning electron microscopy (SEM), fluorescence microscopy, FTIR analysis, Calcofluor white staining, electrospray ionization mass spectrometry (ESI-MS), as well as a chitinase digestion assay were successfully used to confirm the discovery of α-chitin within the skeleton of <i>P. arabica</i>. The current finding should make an important contribution to the field of application of this verongiid sponge as a novel renewable source of biologically-active metabolites and chitin, which are important for development of the blue biotechnology especially in marine oriented biomedicine.https://www.mdpi.com/1660-3397/17/2/92chitinscaffoldsbiological materialsdemosponges<i>Pseudoceratina arabica</i> |
| spellingShingle | Lamiaa A. Shaala Hani Z. Asfour Diaa T. A. Youssef Sonia Żółtowska-Aksamitowska Marcin Wysokowski Mikhail Tsurkan Roberta Galli Heike Meissner Iaroslav Petrenko Konstantin Tabachnick Viatcheslav N. Ivanenko Nicole Bechmann Lyubov V. Muzychka Oleg B. Smolii Rajko Martinović Yvonne Joseph Teofil Jesionowski Hermann Ehrlich New Source of 3D Chitin Scaffolds: The Red Sea Demosponge <i>Pseudoceratina arabica</i> (Pseudoceratinidae, Verongiida) Marine Drugs chitin scaffolds biological materials demosponges <i>Pseudoceratina arabica</i> |
| title | New Source of 3D Chitin Scaffolds: The Red Sea Demosponge <i>Pseudoceratina arabica</i> (Pseudoceratinidae, Verongiida) |
| title_full | New Source of 3D Chitin Scaffolds: The Red Sea Demosponge <i>Pseudoceratina arabica</i> (Pseudoceratinidae, Verongiida) |
| title_fullStr | New Source of 3D Chitin Scaffolds: The Red Sea Demosponge <i>Pseudoceratina arabica</i> (Pseudoceratinidae, Verongiida) |
| title_full_unstemmed | New Source of 3D Chitin Scaffolds: The Red Sea Demosponge <i>Pseudoceratina arabica</i> (Pseudoceratinidae, Verongiida) |
| title_short | New Source of 3D Chitin Scaffolds: The Red Sea Demosponge <i>Pseudoceratina arabica</i> (Pseudoceratinidae, Verongiida) |
| title_sort | new source of 3d chitin scaffolds the red sea demosponge i pseudoceratina arabica i pseudoceratinidae verongiida |
| topic | chitin scaffolds biological materials demosponges <i>Pseudoceratina arabica</i> |
| url | https://www.mdpi.com/1660-3397/17/2/92 |
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