DHEA supplementation in ovariectomized rats reduces impaired glucose-stimulated insulin secretion induced by a high-fat diet
Dehydroepiandrosterone (DHEA) and the dehydroepiandrosterone sulfate (DHEA-S) are steroids produced mainly by the adrenal cortex. There is evidence from both human and animal models suggesting beneficial effects of these steroids for obesity, diabetes mellitus, hypertension, and osteoporosis, condit...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | FEBS Open Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211546314000060 |
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| author | Katherine Veras Felipe Natali Almeida Renato Tadeu Nachbar Daniel Simões de Jesus João Paulo Camporez Ângelo Rafael Carpinelli Julia H. Goedecke Carla Roberta de Oliveira Carvalho |
| author_facet | Katherine Veras Felipe Natali Almeida Renato Tadeu Nachbar Daniel Simões de Jesus João Paulo Camporez Ângelo Rafael Carpinelli Julia H. Goedecke Carla Roberta de Oliveira Carvalho |
| author_sort | Katherine Veras |
| collection | DOAJ |
| description | Dehydroepiandrosterone (DHEA) and the dehydroepiandrosterone sulfate (DHEA-S) are steroids produced mainly by the adrenal cortex. There is evidence from both human and animal models suggesting beneficial effects of these steroids for obesity, diabetes mellitus, hypertension, and osteoporosis, conditions associated with the post-menopausal period. Accordingly, we hypothesized that DHEA supplementation in ovariectomized (OVX) female rats fed a high-fat diet would maintain glucose-induced insulin secretion (GSIS) and pancreatic islet function. OVX resulted in a 30% enlargement of the pancreatic islets area compared to the control rats, which was accompanied by a 50% reduction in the phosphorylation of AKT protein in the pancreatic islets. However, a short-term high-fat diet induced insulin resistance, accompanied by impaired GSIS in isolated pancreatic islets. These effects were reversed by DHEA treatment, with improved insulin sensitivity to levels similar to the control group, and with increased serine phosphorylation of the AKT protein. These data confirm the protective effect of DHEA on the endocrine pancreas in a situation of diet-induced overweight and low estrogen concentrations, a phenotype similar to that of the post-menopausal period. |
| first_indexed | 2024-12-12T06:15:26Z |
| format | Article |
| id | doaj.art-e85fa539517a4037bd07a7285bdfd91a |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-12-12T06:15:26Z |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-e85fa539517a4037bd07a7285bdfd91a2022-12-22T00:35:03ZengWileyFEBS Open Bio2211-54632014-01-014C14114610.1016/j.fob.2014.01.005DHEA supplementation in ovariectomized rats reduces impaired glucose-stimulated insulin secretion induced by a high-fat dietKatherine Veras0Felipe Natali Almeida1Renato Tadeu Nachbar2Daniel Simões de Jesus3João Paulo Camporez4Ângelo Rafael Carpinelli5Julia H. Goedecke6Carla Roberta de Oliveira Carvalho7Department of Physiology and Biophysics, ICB 1, USP, São Paulo, SP, BrazilDepartment of Physiology and Biophysics, ICB 1, USP, São Paulo, SP, BrazilDepartment of Physiology and Biophysics, ICB 1, USP, São Paulo, SP, BrazilDepartment of Physiology and Biophysics, ICB 1, USP, São Paulo, SP, BrazilDepartment of Physiology and Biophysics, ICB 1, USP, São Paulo, SP, BrazilDepartment of Physiology and Biophysics, ICB 1, USP, São Paulo, SP, BrazilSouth African Medical Research Council and Department of Human Biology, University of Cape Town, Cape Town, South AfricaDepartment of Physiology and Biophysics, ICB 1, USP, São Paulo, SP, BrazilDehydroepiandrosterone (DHEA) and the dehydroepiandrosterone sulfate (DHEA-S) are steroids produced mainly by the adrenal cortex. There is evidence from both human and animal models suggesting beneficial effects of these steroids for obesity, diabetes mellitus, hypertension, and osteoporosis, conditions associated with the post-menopausal period. Accordingly, we hypothesized that DHEA supplementation in ovariectomized (OVX) female rats fed a high-fat diet would maintain glucose-induced insulin secretion (GSIS) and pancreatic islet function. OVX resulted in a 30% enlargement of the pancreatic islets area compared to the control rats, which was accompanied by a 50% reduction in the phosphorylation of AKT protein in the pancreatic islets. However, a short-term high-fat diet induced insulin resistance, accompanied by impaired GSIS in isolated pancreatic islets. These effects were reversed by DHEA treatment, with improved insulin sensitivity to levels similar to the control group, and with increased serine phosphorylation of the AKT protein. These data confirm the protective effect of DHEA on the endocrine pancreas in a situation of diet-induced overweight and low estrogen concentrations, a phenotype similar to that of the post-menopausal period.http://www.sciencedirect.com/science/article/pii/S2211546314000060High fat dietMenopausePancreatic isletsInsulin sensitivityInsulin secretionp-Akt/Akt |
| spellingShingle | Katherine Veras Felipe Natali Almeida Renato Tadeu Nachbar Daniel Simões de Jesus João Paulo Camporez Ângelo Rafael Carpinelli Julia H. Goedecke Carla Roberta de Oliveira Carvalho DHEA supplementation in ovariectomized rats reduces impaired glucose-stimulated insulin secretion induced by a high-fat diet FEBS Open Bio High fat diet Menopause Pancreatic islets Insulin sensitivity Insulin secretion p-Akt/Akt |
| title | DHEA supplementation in ovariectomized rats reduces impaired glucose-stimulated insulin secretion induced by a high-fat diet |
| title_full | DHEA supplementation in ovariectomized rats reduces impaired glucose-stimulated insulin secretion induced by a high-fat diet |
| title_fullStr | DHEA supplementation in ovariectomized rats reduces impaired glucose-stimulated insulin secretion induced by a high-fat diet |
| title_full_unstemmed | DHEA supplementation in ovariectomized rats reduces impaired glucose-stimulated insulin secretion induced by a high-fat diet |
| title_short | DHEA supplementation in ovariectomized rats reduces impaired glucose-stimulated insulin secretion induced by a high-fat diet |
| title_sort | dhea supplementation in ovariectomized rats reduces impaired glucose stimulated insulin secretion induced by a high fat diet |
| topic | High fat diet Menopause Pancreatic islets Insulin sensitivity Insulin secretion p-Akt/Akt |
| url | http://www.sciencedirect.com/science/article/pii/S2211546314000060 |
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