Clade-Specific Quantitative Analysis of Photosynthetic Gene Expression in Prochlorococcus.
Newly designed primers targeting rbcL (CO2 fixation), psbA (photosystem II) and rnpB (reference) genes were used in qRT-PCR assays to assess the photosynthetic capability of natural communities of Prochlorococcus, the most abundant photosynthetic organism on Earth and a major contributor to primary...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4526520?pdf=render |
| _version_ | 1828446236811722752 |
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| author | María-Carmen Fernández-Pinos Marta Casado Gemma Caballero Erik R Zinser Jordi Dachs Benjamin Piña |
| author_facet | María-Carmen Fernández-Pinos Marta Casado Gemma Caballero Erik R Zinser Jordi Dachs Benjamin Piña |
| author_sort | María-Carmen Fernández-Pinos |
| collection | DOAJ |
| description | Newly designed primers targeting rbcL (CO2 fixation), psbA (photosystem II) and rnpB (reference) genes were used in qRT-PCR assays to assess the photosynthetic capability of natural communities of Prochlorococcus, the most abundant photosynthetic organism on Earth and a major contributor to primary production in oligotrophic oceans. After optimizing sample collection methodology, we analyzed a total of 62 stations from the Malaspina 2010 circumnavigation (including Atlantic, Pacific and Indian Oceans) at three different depths. Sequence and quantitative analyses of the corresponding amplicons showed the presence of high-light (HL) and low-light (LL) Prochlorococcus clades in essentially all 182 samples, with a largely uniform stratification of LL and HL sequences. Synechococcus cross-amplifications were detected by the taxon-specific melting temperatures of the amplicons. Laboratory exposure of Prochlorococcus MED4 (HL) and MIT9313 (LL) strains to organic pollutants (PAHs and organochlorine compounds) showed a decrease of rbcL transcript abundances, and of the rbcL to psbA ratios for both strains. We propose this technique as a convenient assay to evaluate effects of environmental stressors, including pollution, on the oceanic Prochlorococcus photosynthetic function. |
| first_indexed | 2024-12-10T22:09:14Z |
| format | Article |
| id | doaj.art-e8619083ed224baea63a43ae6dff47ef |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T22:09:14Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-e8619083ed224baea63a43ae6dff47ef2022-12-22T01:31:40ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01108e013320710.1371/journal.pone.0133207Clade-Specific Quantitative Analysis of Photosynthetic Gene Expression in Prochlorococcus.María-Carmen Fernández-PinosMarta CasadoGemma CaballeroErik R ZinserJordi DachsBenjamin PiñaNewly designed primers targeting rbcL (CO2 fixation), psbA (photosystem II) and rnpB (reference) genes were used in qRT-PCR assays to assess the photosynthetic capability of natural communities of Prochlorococcus, the most abundant photosynthetic organism on Earth and a major contributor to primary production in oligotrophic oceans. After optimizing sample collection methodology, we analyzed a total of 62 stations from the Malaspina 2010 circumnavigation (including Atlantic, Pacific and Indian Oceans) at three different depths. Sequence and quantitative analyses of the corresponding amplicons showed the presence of high-light (HL) and low-light (LL) Prochlorococcus clades in essentially all 182 samples, with a largely uniform stratification of LL and HL sequences. Synechococcus cross-amplifications were detected by the taxon-specific melting temperatures of the amplicons. Laboratory exposure of Prochlorococcus MED4 (HL) and MIT9313 (LL) strains to organic pollutants (PAHs and organochlorine compounds) showed a decrease of rbcL transcript abundances, and of the rbcL to psbA ratios for both strains. We propose this technique as a convenient assay to evaluate effects of environmental stressors, including pollution, on the oceanic Prochlorococcus photosynthetic function.http://europepmc.org/articles/PMC4526520?pdf=render |
| spellingShingle | María-Carmen Fernández-Pinos Marta Casado Gemma Caballero Erik R Zinser Jordi Dachs Benjamin Piña Clade-Specific Quantitative Analysis of Photosynthetic Gene Expression in Prochlorococcus. PLoS ONE |
| title | Clade-Specific Quantitative Analysis of Photosynthetic Gene Expression in Prochlorococcus. |
| title_full | Clade-Specific Quantitative Analysis of Photosynthetic Gene Expression in Prochlorococcus. |
| title_fullStr | Clade-Specific Quantitative Analysis of Photosynthetic Gene Expression in Prochlorococcus. |
| title_full_unstemmed | Clade-Specific Quantitative Analysis of Photosynthetic Gene Expression in Prochlorococcus. |
| title_short | Clade-Specific Quantitative Analysis of Photosynthetic Gene Expression in Prochlorococcus. |
| title_sort | clade specific quantitative analysis of photosynthetic gene expression in prochlorococcus |
| url | http://europepmc.org/articles/PMC4526520?pdf=render |
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