Serum polychlorinated biphenyl levels and circulating miRNAs in non-obese women with and without polycystic ovary syndrome
IntroductionPolychlorinated biphenyls (PCBs), organic lipophilic pollutants that accumulate through diet and increase with age, have been associated with polycystic ovary syndrome (PCOS) and shown to affect microRNA (miRNA) expression. This work aimed to determine if PCBs were associated with circul...
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2023.1233484/full |
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| author | Edwina Brennan Alexandra E. Butler Daniel S. Drage Daniel S. Drage Thozhukat Sathyapalan Stephen L. Atkin |
| author_facet | Edwina Brennan Alexandra E. Butler Daniel S. Drage Daniel S. Drage Thozhukat Sathyapalan Stephen L. Atkin |
| author_sort | Edwina Brennan |
| collection | DOAJ |
| description | IntroductionPolychlorinated biphenyls (PCBs), organic lipophilic pollutants that accumulate through diet and increase with age, have been associated with polycystic ovary syndrome (PCOS) and shown to affect microRNA (miRNA) expression. This work aimed to determine if PCBs were associated with circulating miRNAs and whether there were any correlations with serum PCB/miRNA levels and hormonal changes.Methods29 non-obese PCOS and 29 healthy control women, with similar age and body mass index (BMI), had their serum miRNAs measured together with 7 indicator PCBs (PCB28, PCB52, PCB101, PCB118, PCB138, PCB153, PCB180) using high resolution gas chromatography coupled with high resolution mass spectrometry.ResultsIn the combined study cohort, four miRNAs (hsa-miR-139-5p, hsa-miR-424-5p, hsa-miR-195-5p, hsa-miR-335-5p) correlated with PCBs, but none correlated with metabolic parameters. hsa-miR-335-5p correlated with FSH. When stratified, 25 miRNAs correlated with PCBs in controls compared to only one (hsa-miR-193a-5p) in PCOS; none of these miRNAs correlated with the metabolic parameters of BMI, insulin resistance, or inflammation (C-reactive protein, CRP). However, of these 25 miRNAs in controls, hsa-miR-26a-5p, hsa-miR-193a-5p, hsa-miR-2110 and hsa-miR-195-5p positively correlated with luteinizing hormone (LH), hsa-miR-99b-5p and hsa-miR-146b-5p correlated with estradiol, hsa-miR-193a-5p correlated with progesterone, hsa-miR-195-5p correlated with follicle stimulating hormone (FSH), and hsa-miR-139-5p and hsa-miR-146b-5p negatively correlated with anti-müllerian hormone (AMH) (all p<0.05). hsa-miR-193a-5p in PCOS cases correlated with estradiol.ConclusionIn this cohort of women, with no difference in age and BMI, and with similar PCB levels, the miRNAs correlating to PCBs associated with menstrual cycle factors in healthy menstruating controls versus the anovulatory PCOS subjects. The PCB-associated miRNAs did not correlate with non-reproductive hormonal and metabolic parameters. This suggests that PCB effects on miRNAs may result in changes to the hypothalamo-ovarian axis that may thus affect fertility. |
| first_indexed | 2024-03-11T23:51:38Z |
| format | Article |
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| language | English |
| last_indexed | 2024-03-11T23:51:38Z |
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| spelling | doaj.art-e8639890989e412c91bad03a64a9fb432023-09-19T07:29:21ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922023-09-011410.3389/fendo.2023.12334841233484Serum polychlorinated biphenyl levels and circulating miRNAs in non-obese women with and without polycystic ovary syndromeEdwina Brennan0Alexandra E. Butler1Daniel S. Drage2Daniel S. Drage3Thozhukat Sathyapalan4Stephen L. Atkin5School of Medicine, Royal College of Surgeons in Ireland-Medical University of Bahrain, Busaiteen, BahrainSchool of Medicine, Royal College of Surgeons in Ireland-Medical University of Bahrain, Busaiteen, BahrainSchool of Geography, Earth and Environmental Sciences, University of Birmingham, Birmingham, United KingdomQueensland Alliance for Environmental Health Sciences, The University of Queensland, Brisbane, QLD, AustraliaHull York Medical School, University of Hull, Hull, United KingdomSchool of Medicine, Royal College of Surgeons in Ireland-Medical University of Bahrain, Busaiteen, BahrainIntroductionPolychlorinated biphenyls (PCBs), organic lipophilic pollutants that accumulate through diet and increase with age, have been associated with polycystic ovary syndrome (PCOS) and shown to affect microRNA (miRNA) expression. This work aimed to determine if PCBs were associated with circulating miRNAs and whether there were any correlations with serum PCB/miRNA levels and hormonal changes.Methods29 non-obese PCOS and 29 healthy control women, with similar age and body mass index (BMI), had their serum miRNAs measured together with 7 indicator PCBs (PCB28, PCB52, PCB101, PCB118, PCB138, PCB153, PCB180) using high resolution gas chromatography coupled with high resolution mass spectrometry.ResultsIn the combined study cohort, four miRNAs (hsa-miR-139-5p, hsa-miR-424-5p, hsa-miR-195-5p, hsa-miR-335-5p) correlated with PCBs, but none correlated with metabolic parameters. hsa-miR-335-5p correlated with FSH. When stratified, 25 miRNAs correlated with PCBs in controls compared to only one (hsa-miR-193a-5p) in PCOS; none of these miRNAs correlated with the metabolic parameters of BMI, insulin resistance, or inflammation (C-reactive protein, CRP). However, of these 25 miRNAs in controls, hsa-miR-26a-5p, hsa-miR-193a-5p, hsa-miR-2110 and hsa-miR-195-5p positively correlated with luteinizing hormone (LH), hsa-miR-99b-5p and hsa-miR-146b-5p correlated with estradiol, hsa-miR-193a-5p correlated with progesterone, hsa-miR-195-5p correlated with follicle stimulating hormone (FSH), and hsa-miR-139-5p and hsa-miR-146b-5p negatively correlated with anti-müllerian hormone (AMH) (all p<0.05). hsa-miR-193a-5p in PCOS cases correlated with estradiol.ConclusionIn this cohort of women, with no difference in age and BMI, and with similar PCB levels, the miRNAs correlating to PCBs associated with menstrual cycle factors in healthy menstruating controls versus the anovulatory PCOS subjects. The PCB-associated miRNAs did not correlate with non-reproductive hormonal and metabolic parameters. This suggests that PCB effects on miRNAs may result in changes to the hypothalamo-ovarian axis that may thus affect fertility.https://www.frontiersin.org/articles/10.3389/fendo.2023.1233484/fullmicroRNA (miRNA)polychlorinated biphenyls (PCBs)polycystic ovary syndrome (PCOS)endocrine disruptorsorganic pollutants |
| spellingShingle | Edwina Brennan Alexandra E. Butler Daniel S. Drage Daniel S. Drage Thozhukat Sathyapalan Stephen L. Atkin Serum polychlorinated biphenyl levels and circulating miRNAs in non-obese women with and without polycystic ovary syndrome Frontiers in Endocrinology microRNA (miRNA) polychlorinated biphenyls (PCBs) polycystic ovary syndrome (PCOS) endocrine disruptors organic pollutants |
| title | Serum polychlorinated biphenyl levels and circulating miRNAs in non-obese women with and without polycystic ovary syndrome |
| title_full | Serum polychlorinated biphenyl levels and circulating miRNAs in non-obese women with and without polycystic ovary syndrome |
| title_fullStr | Serum polychlorinated biphenyl levels and circulating miRNAs in non-obese women with and without polycystic ovary syndrome |
| title_full_unstemmed | Serum polychlorinated biphenyl levels and circulating miRNAs in non-obese women with and without polycystic ovary syndrome |
| title_short | Serum polychlorinated biphenyl levels and circulating miRNAs in non-obese women with and without polycystic ovary syndrome |
| title_sort | serum polychlorinated biphenyl levels and circulating mirnas in non obese women with and without polycystic ovary syndrome |
| topic | microRNA (miRNA) polychlorinated biphenyls (PCBs) polycystic ovary syndrome (PCOS) endocrine disruptors organic pollutants |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2023.1233484/full |
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