Gut microbiota in the early stage of Crohn’s disease has unique characteristics
Abstract Background Emerging evidence suggests that gut microbiota plays a predominant role in Crohn’s disease (CD). However, the microbiome alterations in the early stage of CD patients still remain unclear. The present study aimed to identify dysbacteriosis in patients with early CD and explore sp...
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| Format: | Article |
| Language: | English |
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BMC
2022-12-01
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| Series: | Gut Pathogens |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13099-022-00521-0 |
| _version_ | 1798005035220598784 |
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| author | Xianzong Ma Xiaojuan Lu Wenyu Zhang Lang Yang Dezhi Wang Junfeng Xu Yan Jia Xin Wang Hui Xie Shu Li Mingjie Zhang Yuqi He Peng Jin Jianqiu Sheng |
| author_facet | Xianzong Ma Xiaojuan Lu Wenyu Zhang Lang Yang Dezhi Wang Junfeng Xu Yan Jia Xin Wang Hui Xie Shu Li Mingjie Zhang Yuqi He Peng Jin Jianqiu Sheng |
| author_sort | Xianzong Ma |
| collection | DOAJ |
| description | Abstract Background Emerging evidence suggests that gut microbiota plays a predominant role in Crohn’s disease (CD). However, the microbiome alterations in the early stage of CD patients still remain unclear. The present study aimed to identify dysbacteriosis in patients with early CD and explore specific gut bacteria related to the progression of CD. Methods This study was nested within a longitudinal prospective Chinese CD cohort, and it included 18 early CD patients, 22 advanced CD patients and 30 healthy controls. The microbiota communities were investigated using high-throughput Illumina HiSeq sequencing targeting the V3–V4 region of 16S ribosomal DNA (rDNA) gene. The relationship between the gut microbiota and clinical characteristics of CD was analyzed. Results Differential microbiota compositions were observed in CD samples (including early and advanced CD samples) and healthy controls samples. Notably, Lachnospiracea_incertae_sedis and Parabacteroides were enriched in the early CD patients, Escherichia/Shigella, Enterococcus and Proteus were enriched in the advanced CD patients, and Roseburia, Gemmiger, Coprococcus, Ruminococcus 2, Butyricicoccus, Dorea, Fusicatenibacter, Anaerostipes, Clostridium IV were enriched in the healthy controls [LDA score (log10) > 2]. Furthermore, Kruskal–Wallis Rank sum test results showed that Blautia, Clostridium IV, Coprococcus, Dorea, Fusicatenibacter continued to significantly decrease in early and advanced CD patients, and Escherichia/Shigella and Proteus continued to significantly increase compared with healthy controls (P < 0.05). The PICRUSt analysis identified 16 remarkably different metabolic pathways [LDA score (log10) > 2]. Some genera were significantly correlated with various clinical parameters, such as fecal calprotectin, erythrocyte sedimentation rate, C-reactive protein, gland reduce, goblet cells decreased, clinical symptoms (P < 0.05). Conclusions Dysbacteriosis occurs in the early stage of CD and is associated with the progression of CD. This data provides a foundation that furthers the understanding of the role of gut microbiota in CD’s pathogenesis. |
| first_indexed | 2024-04-11T12:33:58Z |
| format | Article |
| id | doaj.art-e86cf7179a474b0aaa8c5e72b4ec1662 |
| institution | Directory Open Access Journal |
| issn | 1757-4749 |
| language | English |
| last_indexed | 2024-04-11T12:33:58Z |
| publishDate | 2022-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Gut Pathogens |
| spelling | doaj.art-e86cf7179a474b0aaa8c5e72b4ec16622022-12-22T04:23:41ZengBMCGut Pathogens1757-47492022-12-0114111310.1186/s13099-022-00521-0Gut microbiota in the early stage of Crohn’s disease has unique characteristicsXianzong Ma0Xiaojuan Lu1Wenyu Zhang2Lang Yang3Dezhi Wang4Junfeng Xu5Yan Jia6Xin Wang7Hui Xie8Shu Li9Mingjie Zhang10Yuqi He11Peng Jin12Jianqiu Sheng13Medical School of Chinese PLADepartment of Gastroenterology, The Seventh Medical Center of Chinese PLA General HospitalCapital Medical UniversityDepartment of Gastroenterology, The Seventh Medical Center of Chinese PLA General HospitalMedical School of Chinese PLASenior Department of Gastroenterology, The First Medical Center of Chinese PLA General HospitalDepartment of Gastroenterology, The Seventh Medical Center of Chinese PLA General HospitalDepartment of Gastroenterology, The Seventh Medical Center of Chinese PLA General HospitalDepartment of Gastroenterology, The Seventh Medical Center of Chinese PLA General HospitalDepartment of Gastroenterology, The Seventh Medical Center of Chinese PLA General HospitalDepartment of Gastroenterology, The Seventh Medical Center of Chinese PLA General HospitalDepartment of Gastroenterology, The Seventh Medical Center of Chinese PLA General HospitalDepartment of Gastroenterology, The Seventh Medical Center of Chinese PLA General HospitalMedical School of Chinese PLAAbstract Background Emerging evidence suggests that gut microbiota plays a predominant role in Crohn’s disease (CD). However, the microbiome alterations in the early stage of CD patients still remain unclear. The present study aimed to identify dysbacteriosis in patients with early CD and explore specific gut bacteria related to the progression of CD. Methods This study was nested within a longitudinal prospective Chinese CD cohort, and it included 18 early CD patients, 22 advanced CD patients and 30 healthy controls. The microbiota communities were investigated using high-throughput Illumina HiSeq sequencing targeting the V3–V4 region of 16S ribosomal DNA (rDNA) gene. The relationship between the gut microbiota and clinical characteristics of CD was analyzed. Results Differential microbiota compositions were observed in CD samples (including early and advanced CD samples) and healthy controls samples. Notably, Lachnospiracea_incertae_sedis and Parabacteroides were enriched in the early CD patients, Escherichia/Shigella, Enterococcus and Proteus were enriched in the advanced CD patients, and Roseburia, Gemmiger, Coprococcus, Ruminococcus 2, Butyricicoccus, Dorea, Fusicatenibacter, Anaerostipes, Clostridium IV were enriched in the healthy controls [LDA score (log10) > 2]. Furthermore, Kruskal–Wallis Rank sum test results showed that Blautia, Clostridium IV, Coprococcus, Dorea, Fusicatenibacter continued to significantly decrease in early and advanced CD patients, and Escherichia/Shigella and Proteus continued to significantly increase compared with healthy controls (P < 0.05). The PICRUSt analysis identified 16 remarkably different metabolic pathways [LDA score (log10) > 2]. Some genera were significantly correlated with various clinical parameters, such as fecal calprotectin, erythrocyte sedimentation rate, C-reactive protein, gland reduce, goblet cells decreased, clinical symptoms (P < 0.05). Conclusions Dysbacteriosis occurs in the early stage of CD and is associated with the progression of CD. This data provides a foundation that furthers the understanding of the role of gut microbiota in CD’s pathogenesis.https://doi.org/10.1186/s13099-022-00521-0Crohn’s diseaseEarly diagnosisGut microbiota16S rDNADysbacteriosis |
| spellingShingle | Xianzong Ma Xiaojuan Lu Wenyu Zhang Lang Yang Dezhi Wang Junfeng Xu Yan Jia Xin Wang Hui Xie Shu Li Mingjie Zhang Yuqi He Peng Jin Jianqiu Sheng Gut microbiota in the early stage of Crohn’s disease has unique characteristics Gut Pathogens Crohn’s disease Early diagnosis Gut microbiota 16S rDNA Dysbacteriosis |
| title | Gut microbiota in the early stage of Crohn’s disease has unique characteristics |
| title_full | Gut microbiota in the early stage of Crohn’s disease has unique characteristics |
| title_fullStr | Gut microbiota in the early stage of Crohn’s disease has unique characteristics |
| title_full_unstemmed | Gut microbiota in the early stage of Crohn’s disease has unique characteristics |
| title_short | Gut microbiota in the early stage of Crohn’s disease has unique characteristics |
| title_sort | gut microbiota in the early stage of crohn s disease has unique characteristics |
| topic | Crohn’s disease Early diagnosis Gut microbiota 16S rDNA Dysbacteriosis |
| url | https://doi.org/10.1186/s13099-022-00521-0 |
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