The identification and differentiation of the Candida parapsilosis complex species by polymerase chain reaction-restriction fragment length polymorphism of the internal transcribed spacer region of the rDNA
Currently, it is accepted that there are three species that were formerly grouped under Candida parapsilosis: C. para- psilosis sensu stricto, Candida orthopsilosis, andCandida metapsilosis. In fact, the antifungal susceptibility profiles and distinct virulence attributes demonstrate the differences...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2016-04-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016000400267&lng=en&tlng=en |
| _version_ | 1797757007789293568 |
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| author | Leonardo Silva Barbedo Maria Helena Galdino Figueiredo-Carvalho Mauro de Medeiros Muniz Rosely Maria Zancopé-Oliveira |
| author_facet | Leonardo Silva Barbedo Maria Helena Galdino Figueiredo-Carvalho Mauro de Medeiros Muniz Rosely Maria Zancopé-Oliveira |
| author_sort | Leonardo Silva Barbedo |
| collection | DOAJ |
| description | Currently, it is accepted that there are three species that were formerly grouped under Candida parapsilosis: C. para- psilosis sensu stricto, Candida orthopsilosis, andCandida metapsilosis. In fact, the antifungal susceptibility profiles and distinct virulence attributes demonstrate the differences in these nosocomial pathogens. An accurate, fast, and economical identification of fungal species has been the main goal in mycology. In the present study, we searched sequences that were available in the GenBank database in order to identify the complete sequence for the internal transcribed spacer (ITS)1-5.8S-ITS2 region, which is comprised of the forward and reverse primers ITS1 and ITS4. Subsequently, an in silico polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was performed to differentiate the C. parapsilosis complex species. Ninety-eight clinical isolates from patients with fungaemia were submitted for analysis, where 59 isolates were identified as C. parapsilosis sensu stricto, 37 were identified as C. orthopsilosis, and two were identified as C. metapsilosis. PCR-RFLP quickly and accurately identified C. parapsilosis complex species, making this method an alternative and routine identification system for use in clinical mycology laboratories. |
| first_indexed | 2024-03-12T18:09:48Z |
| format | Article |
| id | doaj.art-e874557dd63a42e2b2b0b959c91e165b |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:09:48Z |
| publishDate | 2016-04-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-e874557dd63a42e2b2b0b959c91e165b2023-08-02T09:16:37ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602016-04-01111426727010.1590/0074-02760150466S0074-02762016000400267The identification and differentiation of the Candida parapsilosis complex species by polymerase chain reaction-restriction fragment length polymorphism of the internal transcribed spacer region of the rDNALeonardo Silva BarbedoMaria Helena Galdino Figueiredo-CarvalhoMauro de Medeiros MunizRosely Maria Zancopé-OliveiraCurrently, it is accepted that there are three species that were formerly grouped under Candida parapsilosis: C. para- psilosis sensu stricto, Candida orthopsilosis, andCandida metapsilosis. In fact, the antifungal susceptibility profiles and distinct virulence attributes demonstrate the differences in these nosocomial pathogens. An accurate, fast, and economical identification of fungal species has been the main goal in mycology. In the present study, we searched sequences that were available in the GenBank database in order to identify the complete sequence for the internal transcribed spacer (ITS)1-5.8S-ITS2 region, which is comprised of the forward and reverse primers ITS1 and ITS4. Subsequently, an in silico polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was performed to differentiate the C. parapsilosis complex species. Ninety-eight clinical isolates from patients with fungaemia were submitted for analysis, where 59 isolates were identified as C. parapsilosis sensu stricto, 37 were identified as C. orthopsilosis, and two were identified as C. metapsilosis. PCR-RFLP quickly and accurately identified C. parapsilosis complex species, making this method an alternative and routine identification system for use in clinical mycology laboratories.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016000400267&lng=en&tlng=enCandida parapsilosis complexPCR-RFLPfungaemia |
| spellingShingle | Leonardo Silva Barbedo Maria Helena Galdino Figueiredo-Carvalho Mauro de Medeiros Muniz Rosely Maria Zancopé-Oliveira The identification and differentiation of the Candida parapsilosis complex species by polymerase chain reaction-restriction fragment length polymorphism of the internal transcribed spacer region of the rDNA Memorias do Instituto Oswaldo Cruz Candida parapsilosis complex PCR-RFLP fungaemia |
| title | The identification and differentiation of the Candida parapsilosis complex species by polymerase chain reaction-restriction fragment length polymorphism of the internal transcribed spacer region of the rDNA |
| title_full | The identification and differentiation of the Candida parapsilosis complex species by polymerase chain reaction-restriction fragment length polymorphism of the internal transcribed spacer region of the rDNA |
| title_fullStr | The identification and differentiation of the Candida parapsilosis complex species by polymerase chain reaction-restriction fragment length polymorphism of the internal transcribed spacer region of the rDNA |
| title_full_unstemmed | The identification and differentiation of the Candida parapsilosis complex species by polymerase chain reaction-restriction fragment length polymorphism of the internal transcribed spacer region of the rDNA |
| title_short | The identification and differentiation of the Candida parapsilosis complex species by polymerase chain reaction-restriction fragment length polymorphism of the internal transcribed spacer region of the rDNA |
| title_sort | identification and differentiation of the candida parapsilosis complex species by polymerase chain reaction restriction fragment length polymorphism of the internal transcribed spacer region of the rdna |
| topic | Candida parapsilosis complex PCR-RFLP fungaemia |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016000400267&lng=en&tlng=en |
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