LINC00963 Promotes Cancer Stemness, Metastasis, and Drug Resistance in Head and Neck Carcinomas via ABCB5 Regulation
Accumulating studies have indicated that long non-coding RNAs (lncRNAs) participate in the regulation of cancer stem cells (CSCs), which are crucial in tumor initiation, metastasis, relapse, and therapy resistance. In the current study, RT-PCR analysis was employed to evaluate the expression of LINC...
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MDPI AG
2020-04-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/12/5/1073 |
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| author | Shiao-Pieng Lee Pei-Ling Hsieh Chih-Yuan Fang Pei-Ming Chu Yi-Wen Liao Chuan-Hang Yu Cheng-Chia Yu Lo-Lin Tsai |
| author_facet | Shiao-Pieng Lee Pei-Ling Hsieh Chih-Yuan Fang Pei-Ming Chu Yi-Wen Liao Chuan-Hang Yu Cheng-Chia Yu Lo-Lin Tsai |
| author_sort | Shiao-Pieng Lee |
| collection | DOAJ |
| description | Accumulating studies have indicated that long non-coding RNAs (lncRNAs) participate in the regulation of cancer stem cells (CSCs), which are crucial in tumor initiation, metastasis, relapse, and therapy resistance. In the current study, RT-PCR analysis was employed to evaluate the expression of LINC00963 in tumor tissues and oral CSCs. Stemness phenotypes and the expression of CSCs markers in oral cancer cells transfected with sh-LINC00963 were examined. Our results showed that the expression of the lncRNA LINC00963 was up-regulated in oral cancer tissues and CSCs. We found that the downregulation of LINC00963 inhibited CSC hallmarks, such as migration, invasion and colony formation capacity. Moreover, suppression of LINC00963 reduced the activity of stemness marker ALDH1, the percentage of self-renewal, chemoresistance and the expression of multidrug-resistance transporter ABCB5. Most importantly, we demonstrated that knockdown of LINC00963 decreased self-renewal, invasion and colony formation ability via ABCB5. Analysis of TCGA (the Cancer Genome Atlas) datasets suggested that the level of LINC00963 was positively correlated with the expression of the cancer stemness markers (Sox2 and CD44) and drug resistance markers (ABCG2 and ABCB5). Altogether, our results showed that suppression of LINC00963 may be beneficial to inhibit chemoresistance and cancer relapse in oral cancer patients. |
| first_indexed | 2024-03-10T20:13:58Z |
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| id | doaj.art-e877ac8fe7484963883240662a0ae233 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T20:13:58Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-e877ac8fe7484963883240662a0ae2332023-11-19T22:44:44ZengMDPI AGCancers2072-66942020-04-01125107310.3390/cancers12051073LINC00963 Promotes Cancer Stemness, Metastasis, and Drug Resistance in Head and Neck Carcinomas via ABCB5 RegulationShiao-Pieng Lee0Pei-Ling Hsieh1Chih-Yuan Fang2Pei-Ming Chu3Yi-Wen Liao4Chuan-Hang Yu5Cheng-Chia Yu6Lo-Lin Tsai7School of Dentistry, National Defense Medical Center, Taipei 11490, TaiwanDepartment of Anatomy, School of Medicine, China Medical University, Taichung 404, TaiwanSchool of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 110, TaiwanDepartment of Anatomy, School of Medicine, China Medical University, Taichung 404, TaiwanSchool of Dentistry, Chung Shan Medical University, Taichung 40201, TaiwanSchool of Dentistry, Chung Shan Medical University, Taichung 40201, TaiwanSchool of Dentistry, Chung Shan Medical University, Taichung 40201, TaiwanSchool of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 110, TaiwanAccumulating studies have indicated that long non-coding RNAs (lncRNAs) participate in the regulation of cancer stem cells (CSCs), which are crucial in tumor initiation, metastasis, relapse, and therapy resistance. In the current study, RT-PCR analysis was employed to evaluate the expression of LINC00963 in tumor tissues and oral CSCs. Stemness phenotypes and the expression of CSCs markers in oral cancer cells transfected with sh-LINC00963 were examined. Our results showed that the expression of the lncRNA LINC00963 was up-regulated in oral cancer tissues and CSCs. We found that the downregulation of LINC00963 inhibited CSC hallmarks, such as migration, invasion and colony formation capacity. Moreover, suppression of LINC00963 reduced the activity of stemness marker ALDH1, the percentage of self-renewal, chemoresistance and the expression of multidrug-resistance transporter ABCB5. Most importantly, we demonstrated that knockdown of LINC00963 decreased self-renewal, invasion and colony formation ability via ABCB5. Analysis of TCGA (the Cancer Genome Atlas) datasets suggested that the level of LINC00963 was positively correlated with the expression of the cancer stemness markers (Sox2 and CD44) and drug resistance markers (ABCG2 and ABCB5). Altogether, our results showed that suppression of LINC00963 may be beneficial to inhibit chemoresistance and cancer relapse in oral cancer patients.https://www.mdpi.com/2072-6694/12/5/1073head and necklncRNALINC00963cancer stemnesschemoresistanceABCB5 |
| spellingShingle | Shiao-Pieng Lee Pei-Ling Hsieh Chih-Yuan Fang Pei-Ming Chu Yi-Wen Liao Chuan-Hang Yu Cheng-Chia Yu Lo-Lin Tsai LINC00963 Promotes Cancer Stemness, Metastasis, and Drug Resistance in Head and Neck Carcinomas via ABCB5 Regulation Cancers head and neck lncRNA LINC00963 cancer stemness chemoresistance ABCB5 |
| title | LINC00963 Promotes Cancer Stemness, Metastasis, and Drug Resistance in Head and Neck Carcinomas via ABCB5 Regulation |
| title_full | LINC00963 Promotes Cancer Stemness, Metastasis, and Drug Resistance in Head and Neck Carcinomas via ABCB5 Regulation |
| title_fullStr | LINC00963 Promotes Cancer Stemness, Metastasis, and Drug Resistance in Head and Neck Carcinomas via ABCB5 Regulation |
| title_full_unstemmed | LINC00963 Promotes Cancer Stemness, Metastasis, and Drug Resistance in Head and Neck Carcinomas via ABCB5 Regulation |
| title_short | LINC00963 Promotes Cancer Stemness, Metastasis, and Drug Resistance in Head and Neck Carcinomas via ABCB5 Regulation |
| title_sort | linc00963 promotes cancer stemness metastasis and drug resistance in head and neck carcinomas via abcb5 regulation |
| topic | head and neck lncRNA LINC00963 cancer stemness chemoresistance ABCB5 |
| url | https://www.mdpi.com/2072-6694/12/5/1073 |
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