An insight into the transmission role of insect vectors based on the examination of gene characteristics of African swine fever virus originated from non-blood sucking flies in pig farm environments
Abstract Background Insect vector transmitted pathogens from contaminated environments are a key potential risk for public health. Meanwhile, transmission by non-blood sucking flies needs to be considered. Sequencing and phylogenetic tree analyses were used to study African swine fever virus (ASFV)...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2020-07-01
|
| Series: | BMC Veterinary Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12917-020-02420-5 |
| _version_ | 1819204791869374464 |
|---|---|
| author | Jinling Liu Gen Lu Yuesong Cui Shu Wei Tongqing An Guoshun Shen Zeliang Chen |
| author_facet | Jinling Liu Gen Lu Yuesong Cui Shu Wei Tongqing An Guoshun Shen Zeliang Chen |
| author_sort | Jinling Liu |
| collection | DOAJ |
| description | Abstract Background Insect vector transmitted pathogens from contaminated environments are a key potential risk for public health. Meanwhile, transmission by non-blood sucking flies needs to be considered. Sequencing and phylogenetic tree analyses were used to study African swine fever virus (ASFV) genes derived from flies collected from pig farms that were infected with ASFV. The major differential genes were analyzed the encoded proteins, particularly their conformation, physico-chemical features, and interactions identified by immunophenotyping. Results Results showed that the ASFV p72 and D117L genes from these non-blood sucking flies identified by morphology have high sequence similarity from ASFV genotype II strains, however, A179L is found in an independent cluster, with five amino acid substitutions; four of which are in a continuous sequence. Moreover, the binding of a BH3 peptide into a surface groove formed by α-helices of ASFV A179L from the non-blood sucking flies is consistent with that of representative ASFV genotype II strains, Georgia/2007.They only differ in the direction of spatial interaction of six conserved amino residues. Many hydrophilic amino residues are located at the canonical ligand-binding groove of A179L from flies, with hydrophobic amino residues located at the corresponding positions in A179L of the Georgia/2007.Furthermore, analysis of protein interactions by immunophenotyping revealed that both A179Ls have similar roles in regulating autophagy and apoptosis. Conclusions In conclusion, the main genes that differ between ASFV from flies and Georgia/2007 were similar in structure and protein interaction, while exhibiting differences in physico-chemical features and amino acid variations. Understanding the mechanical transmission characteristics of non-blood sucking flies is important. |
| first_indexed | 2024-12-23T04:41:26Z |
| format | Article |
| id | doaj.art-e87bb9515bcf4224a637f24a5d7ca5c7 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-12-23T04:41:26Z |
| publishDate | 2020-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-e87bb9515bcf4224a637f24a5d7ca5c72022-12-21T17:59:46ZengBMCBMC Veterinary Research1746-61482020-07-011611810.1186/s12917-020-02420-5An insight into the transmission role of insect vectors based on the examination of gene characteristics of African swine fever virus originated from non-blood sucking flies in pig farm environmentsJinling Liu0Gen Lu1Yuesong Cui2Shu Wei3Tongqing An4Guoshun Shen5Zeliang Chen6Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, College of Animal Science & Veterinary Medicine, Shenyang Agricultural UniversityKey Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, College of Animal Science & Veterinary Medicine, Shenyang Agricultural UniversityKey Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, College of Animal Science & Veterinary Medicine, Shenyang Agricultural UniversityThe Preventive Center of Animal Disease of Liaoning ProvinceHarbin Veterinary Research Institute of Chinese Academy of Agricultural SciencesKey Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, College of Animal Science & Veterinary Medicine, Shenyang Agricultural UniversityKey Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, College of Animal Science & Veterinary Medicine, Shenyang Agricultural UniversityAbstract Background Insect vector transmitted pathogens from contaminated environments are a key potential risk for public health. Meanwhile, transmission by non-blood sucking flies needs to be considered. Sequencing and phylogenetic tree analyses were used to study African swine fever virus (ASFV) genes derived from flies collected from pig farms that were infected with ASFV. The major differential genes were analyzed the encoded proteins, particularly their conformation, physico-chemical features, and interactions identified by immunophenotyping. Results Results showed that the ASFV p72 and D117L genes from these non-blood sucking flies identified by morphology have high sequence similarity from ASFV genotype II strains, however, A179L is found in an independent cluster, with five amino acid substitutions; four of which are in a continuous sequence. Moreover, the binding of a BH3 peptide into a surface groove formed by α-helices of ASFV A179L from the non-blood sucking flies is consistent with that of representative ASFV genotype II strains, Georgia/2007.They only differ in the direction of spatial interaction of six conserved amino residues. Many hydrophilic amino residues are located at the canonical ligand-binding groove of A179L from flies, with hydrophobic amino residues located at the corresponding positions in A179L of the Georgia/2007.Furthermore, analysis of protein interactions by immunophenotyping revealed that both A179Ls have similar roles in regulating autophagy and apoptosis. Conclusions In conclusion, the main genes that differ between ASFV from flies and Georgia/2007 were similar in structure and protein interaction, while exhibiting differences in physico-chemical features and amino acid variations. Understanding the mechanical transmission characteristics of non-blood sucking flies is important.http://link.springer.com/article/10.1186/s12917-020-02420-5ASFVNon-blood sucking flyDifferential geneVariantTransmission |
| spellingShingle | Jinling Liu Gen Lu Yuesong Cui Shu Wei Tongqing An Guoshun Shen Zeliang Chen An insight into the transmission role of insect vectors based on the examination of gene characteristics of African swine fever virus originated from non-blood sucking flies in pig farm environments BMC Veterinary Research ASFV Non-blood sucking fly Differential gene Variant Transmission |
| title | An insight into the transmission role of insect vectors based on the examination of gene characteristics of African swine fever virus originated from non-blood sucking flies in pig farm environments |
| title_full | An insight into the transmission role of insect vectors based on the examination of gene characteristics of African swine fever virus originated from non-blood sucking flies in pig farm environments |
| title_fullStr | An insight into the transmission role of insect vectors based on the examination of gene characteristics of African swine fever virus originated from non-blood sucking flies in pig farm environments |
| title_full_unstemmed | An insight into the transmission role of insect vectors based on the examination of gene characteristics of African swine fever virus originated from non-blood sucking flies in pig farm environments |
| title_short | An insight into the transmission role of insect vectors based on the examination of gene characteristics of African swine fever virus originated from non-blood sucking flies in pig farm environments |
| title_sort | insight into the transmission role of insect vectors based on the examination of gene characteristics of african swine fever virus originated from non blood sucking flies in pig farm environments |
| topic | ASFV Non-blood sucking fly Differential gene Variant Transmission |
| url | http://link.springer.com/article/10.1186/s12917-020-02420-5 |
| work_keys_str_mv | AT jinlingliu aninsightintothetransmissionroleofinsectvectorsbasedontheexaminationofgenecharacteristicsofafricanswinefevervirusoriginatedfromnonbloodsuckingfliesinpigfarmenvironments AT genlu aninsightintothetransmissionroleofinsectvectorsbasedontheexaminationofgenecharacteristicsofafricanswinefevervirusoriginatedfromnonbloodsuckingfliesinpigfarmenvironments AT yuesongcui aninsightintothetransmissionroleofinsectvectorsbasedontheexaminationofgenecharacteristicsofafricanswinefevervirusoriginatedfromnonbloodsuckingfliesinpigfarmenvironments AT shuwei aninsightintothetransmissionroleofinsectvectorsbasedontheexaminationofgenecharacteristicsofafricanswinefevervirusoriginatedfromnonbloodsuckingfliesinpigfarmenvironments AT tongqingan aninsightintothetransmissionroleofinsectvectorsbasedontheexaminationofgenecharacteristicsofafricanswinefevervirusoriginatedfromnonbloodsuckingfliesinpigfarmenvironments AT guoshunshen aninsightintothetransmissionroleofinsectvectorsbasedontheexaminationofgenecharacteristicsofafricanswinefevervirusoriginatedfromnonbloodsuckingfliesinpigfarmenvironments AT zeliangchen aninsightintothetransmissionroleofinsectvectorsbasedontheexaminationofgenecharacteristicsofafricanswinefevervirusoriginatedfromnonbloodsuckingfliesinpigfarmenvironments AT jinlingliu insightintothetransmissionroleofinsectvectorsbasedontheexaminationofgenecharacteristicsofafricanswinefevervirusoriginatedfromnonbloodsuckingfliesinpigfarmenvironments AT genlu insightintothetransmissionroleofinsectvectorsbasedontheexaminationofgenecharacteristicsofafricanswinefevervirusoriginatedfromnonbloodsuckingfliesinpigfarmenvironments AT yuesongcui insightintothetransmissionroleofinsectvectorsbasedontheexaminationofgenecharacteristicsofafricanswinefevervirusoriginatedfromnonbloodsuckingfliesinpigfarmenvironments AT shuwei insightintothetransmissionroleofinsectvectorsbasedontheexaminationofgenecharacteristicsofafricanswinefevervirusoriginatedfromnonbloodsuckingfliesinpigfarmenvironments AT tongqingan insightintothetransmissionroleofinsectvectorsbasedontheexaminationofgenecharacteristicsofafricanswinefevervirusoriginatedfromnonbloodsuckingfliesinpigfarmenvironments AT guoshunshen insightintothetransmissionroleofinsectvectorsbasedontheexaminationofgenecharacteristicsofafricanswinefevervirusoriginatedfromnonbloodsuckingfliesinpigfarmenvironments AT zeliangchen insightintothetransmissionroleofinsectvectorsbasedontheexaminationofgenecharacteristicsofafricanswinefevervirusoriginatedfromnonbloodsuckingfliesinpigfarmenvironments |